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An extraction device and its operating method

A technology of extraction device and operation method, applied in the field of medical devices, can solve the problems of slow speed and low cell survival rate

Active Publication Date: 2021-10-15
上海蓓蕊医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The Chinese patent with the publication number CN104357387A discloses a method for isolating human adipose stem cells from human adipose tissue, which first adds a small amount of low-molecular-weight heparin calcium to normal saline as a cleaning solution to effectively remove residual red blood cells and other impurities , and further isolate human adipose stem cells with high purity, but in the process of processing, this method needs to add other cleaning fluids for cleaning, and through centrifugation to improve the purity of adipose stem cells, but it needs to be separated by centrifugation and cultured , which is relatively slow throughout the process, and the cell survival rate is relatively low

Method used

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  • An extraction device and its operating method
  • An extraction device and its operating method
  • An extraction device and its operating method

Examples

Experimental program
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Embodiment 1

[0050] An extraction device, which is used for separating the extracted fat extraction liquid, and separating adipose tissue from the extraction liquid. The device can directly extract the fat extraction liquid from the human body for separation and extraction, and then directly use it clinically. Such as figure 1 and figure 2 As shown, it includes a closed washing and separating drum 1 for storing the extracted fat extraction liquid and performing extraction and separation operations. One end of the washing and separating cylinder 1 is provided with a pipeline injection assembly 2, which is used to inject physiological saline into the washing and separating cylinder 1, to maintain the activity of the extracted fat extraction liquid, and to wash the fat extraction liquid in the washing and separating drum 1 separate. The other end of the washing and separating cylinder 1 is provided with a waste liquid discharge assembly 3 for discharging the waste liquid and impurities gen...

Embodiment 2

[0061] A kind of extracting device, its structure is substantially the same as embodiment 1, the difference lies in the connection mode of injection pipeline 21 and discharge pipeline 31, as Figure 8 and Figure 9 As shown, in embodiment 2, in order to improve the tightness between the pipeline and the washing separation drum 1, the injection pipeline 21 and the discharge pipeline 31 are directly fixed on the socket by heat sealing or gluing. At this time, let the injection pipeline 21 and the discharge pipe 31 directly form an integral structure with the washing separation drum 1, and then make its sealing effect better.

[0062] Such as Figure 8 and Figure 9As shown, in order to facilitate the opening and closing of the pipeline, a pipeline switch 8 is provided on the injection pipeline 21 and the discharge pipeline 31. The injection pipeline 21 and the discharge pipeline 31 are made of soft infusion pipelines, which can be closed and opened by the pipeline switch 8 . ...

Embodiment 3

[0067] A method of operating an extraction device, comprising the steps of:

[0068] S1, pipe plugging; insert the injection pipe 21 and the discharge pipe 31 into the washing separation drum 1 (in the embodiment 1, the injection pipe 21 and the discharge pipe 31 need to be plugged, and in the embodiment 2, the injection pipe 21 and the discharge pipe 31 does not need to be plugged in), then the injection pipeline 21 is connected to the physiological saline bag, and the discharge pipeline 31 is connected to the waste liquid bag 33.

[0069] S2. Exhaust the air; turn the washing and separating cylinder 1 upside down, inject physiological saline into the washing and separating cylinder 1 from the injection pipe 21, and discharge the air in the washing and separating cylinder 1. After the injection is completed, shake it to let some of it adhere to the cylinder. Small air molecules on the walls are expelled.

[0070] S3. Preliminary washing: keep the injection and discharge of p...

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Abstract

The invention discloses an extracting device and an operation method thereof, comprising a washing and separating cylinder and plugs installed at both ends of the washing and separating cylinder, the plugs are provided with a plurality of holes for injecting and extracting adipose tissue and fat extraction liquid. Fat port and a pipeline injection assembly for injecting physiological saline, the plug is provided with a waste liquid discharge assembly for discharging flushed waste water and a functional assembly for assisting the adipose tissue extraction function, and the liposuction head is provided with A filter screen, the filter screen includes a mounting plate fixed to the liposuction head, a number of openings matching the fat ports are provided on the mounting plate, and a filter screen is arranged in the openings. In the whole process, the separation of adipose tissue is carried out in a closed container, and there is no need to add cleaning solution, the cell survival rate is relatively high, and the time for cell separation is short.

Description

technical field [0001] The present invention relates to a medical device and, more particularly, to an extraction device and method of operation thereof. Background technique [0002] Multidirectionally differentiated stem cells can be isolated from human fat suspension, which can differentiate into fat, bone, cartilage, muscle, vascular endothelial, liver, spleen, nerve and other cells, and the stem cells obtained from human fat have differentiation potential The adult stem cells are called human adipose stem cells, which have the following advantages over other stem cells: [0003] 1. Abundant sources and easy access to materials; [0004] 2. Easy to expand and not easy to age; [0005] 3. With paracrine function, it can secrete related factors that promote angiogenesis and promote angiogenesis; [0006] 4. It will not cause strong rejection during transplantation. [0007] The Chinese patent with the publication number CN104357387A discloses a method for isolating hum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/12C12M1/00C12N5/0775
CPCC12M47/04C12N5/0667C12N2509/00
Inventor 彭朗周峰李金洲吴德刚牛朝辉
Owner 上海蓓蕊医疗科技有限公司