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Pear rhizosphere growth promoting acinetobacter junii Lzh-X15 and application thereof

A technology for Acinetobacter jelly and pear tree roots, applied in the field of agricultural microorganisms, can solve the problems of few screening and application, achieve the effects of excellent strain resources, suppress outbreaks, and improve the ecological environment

Inactive Publication Date: 2018-10-19
DEZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few studies on the screening and application of pear rhizosphere growth-promoting bacterial strains

Method used

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  • Pear rhizosphere growth promoting acinetobacter junii Lzh-X15 and application thereof
  • Pear rhizosphere growth promoting acinetobacter junii Lzh-X15 and application thereof
  • Pear rhizosphere growth promoting acinetobacter junii Lzh-X15 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Screening and Identification of Acinetobacter Jones Lzh-X15

[0031] The strain Lzh-X15 was isolated from the rhizosphere soil of pear trees infected with root rot in Decheng District, Dezhou City, Shandong Province. The specific method is as follows:

[0032] Rhizosphere growth-promoting bacteria were screened from plant rhizosphere soil by plate confrontation method. Take rhizosphere soil from the roots of infected plants and send it to the laboratory as soon as possible under low temperature conditions. The rhizosphere soil was put into sterile water for gradient dilution, and then spread on beef extract peptone solid medium, cultured at 30°C for 24 hours, and picked a single colony. Inoculate the obtained single colony on the plant pathogenic bacteria to see if it produces a zone of inhibition and whether it has antagonistic biological activity. The strain Lzh-X15 of the present invention was isolated through a large number of screening tests.

[0033] ...

Embodiment 2

[0036] Embodiment 2 Antagonistic effect of Acinetobacter junii Lzh-X15 of the present invention

[0037] The antagonism of Acinetobacter johnii Lzh-X15 was detected by plate confrontation method. The pear tree root rot pathogen Fusarium moniliforme was inoculated onto a PDA solid medium (200 g of peeled potatoes, 20 g of sucrose, 20 g of agar, 1000 mL of distilled water, natural pH), cultivated at 30° C. for 48 hr and then set aside. Acinetobacter jonesi Lzh-X15 was inoculated into Gaussian solid medium (soluble starch (20g), KNO 3 (1g), K 2 HPO 4 (0.5g), MgSO 4 ·7H 2 O (0.5g), NaCl (0.5g), FeSO 4 ·7H 2O (0.01g), agar 20g, distilled water 1000mL, pH=7.4-7.6) upper 30 ℃ of culture 48hrs are inoculated to pear tree root rot pathogenic bacteria both sides, 30 ℃ continue to cultivate 48-72hr, observe whether to produce antibacterial zone. The result is as figure 1 As shown, the results show that the present invention Acinetobacter johnsoni Lzh-X15 has a strong antagonistic...

Embodiment 3

[0038] Embodiment 3 Acinetobacter acetonii Lzh-X15 microbial bacterial agent and preparation method thereof

[0039] The concrete steps of preparation method are as follows:

[0040] Inoculate the seed liquid of Acinetobacter acanthus Lzh-X15 in the beef extract peptone liquid medium that pH is 7.0, the inoculum size is 2% (v / v), ferment 48h under 28 ℃, 200rpm conditions, then ferment The solution was added to sterilized wheat bran according to the inoculum amount of 10% by mass fraction, cultivated at 30°C for 3 days, and air-dried to obtain the microbial agent of Acinetobacter jonesii Lzh-X15.

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Abstract

The invention relates to pear rhizosphere growth promoting acinetobacter junii Lzh-X15 and application thereof. The preservation number of the acinetobacter junii Lzh-X15 is CGMCC No.13490, and the preservation date is September 23rd, 2016. The invention further relates to application of the acinetobacter junii Lzh-X15, fermentation liquor and microbial fertilizers containing the acinetobacter junii Lzh-X15 in the aspect of preventing and treating pear root rot. A microbial fertilizer prepared by the acinetobacter junii Lzh-X15 disclosed by the invention can obviously reduce the probability that pears are infected by root rot, and furthermore, pear yield and quality are improved.

Description

technical field [0001] The invention relates to a pear tree rhizosphere growth-promoting Acinetobacter ajunii Lzh-X15 and application thereof, in particular to the application of the acinetobacter ajunii Lzh-X15 in preventing and treating root rot of pear trees, and belongs to the field of agricultural microorganisms. Background technique [0002] Pear tree root rot is caused by fungi such as Fusarium moniliforme Sheld, Phytophthora cactorum, Rosellinia necatrix, Helicobasidium mompa, Armillariella tabescens, etc. It is a serious root disease that can cause root rot, yellow leaves, tree weakness, and even the death of the whole plant, seriously affecting the yield and quality of pears. At present, the prevention and control measures of pear tree root rot mainly include a series of methods such as doing a good job in clearing the garden, strengthening the management of pear orchards, strengthening cultivation management, and chemical control. But there is no biological metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C05F17/00C05G3/00C12R1/01
CPCA01N63/10C05F3/00C05F17/00C05G3/00C12N1/20C12N1/205C12R2001/01C05F11/08Y02W30/40
Inventor 李正华王吉华曾强成宋峰周耀旗于家峰冉坤李刻秦师晓喆
Owner DEZHOU UNIV
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