Effective culturing method for body fluid derived tumor cells and application of effective culturing method

Abstract

The invention relates to a culturing method for body fluid derived tumor cells and a tumor medium. The method comprises the steps of enriching body fluid tumor cells; mixing the body fluid tumor cellswith an extracellular matrix; and in the presence of a basic medium, adding corresponding pleural effusion, ascites or cerebrospinal fluid and the like to carry out 3D cell culture. By the method, apatient derived tumor analog (PDTA) can be obtained effectively, and a novel detecting tool is provided for detection of sensitivity of clinical tumor patients to drugs and measurement of in-vitro metabolic stability, metabolic profiling, toxicity and the like of drug candidates.

Description

technical field [0001] The invention relates to the field of culturing tumor cells derived from body fluids, in particular to a culturing method for various pleural effusion, ascites or cerebrospinal fluid tumor cells, a tumor cell culture medium and applications thereof. Background technique [0002] Currently, the models used for basic scientific research in oncology mainly include tumor cell lines and human tumor xenograft models. Among them, the tumor cell line has the advantages of short culture cycle, easy operation, and low cost. The disadvantages are: 1) The tumor cell line is cultured under 2D conditions, which is quite different from the in vivo environment; 2) The genetics of the tumor cell line Background and gene expression profiles are quite different from clinical patient tumor cells. Therefore, tumor cell lines are usually only used as basic exploratory research tools. Human-derived xenograft model (patient-derived xenograft model, PDX model) refers to the ...

AI Technical Summary

Problems solved by technology

Among them, the tumor cell line has the advantages of short culture cycle, easy operation, and low cost. The disadvantages are: 1) The tumor cell line is cultured under 2D conditions, which is quite different from the in vivo environment; 2) The genetics of the tumor cell line Background and gene expression profiles are quite different from clinical patient tumor cells

Its disadvantages are as follows: 1) The culture cycle is long, and it usually takes several months from tumor tissue acquisition to inoculation, subculture, and experiment; 2) It needs special immunodeficiency mice and animal culture places, which are expensive; 3) Experimental animals are sensitive to drugs. Reactivity, etc. are quite different from humans

CN102439135B discloses the culture of stem cell organoids contained in normal epithelial tissues and the 3D culture of tumor cells derived from epithelial adenoma tissue blocks, but does not involve the isolation of tumor cells from body fluids, especially the separation of tumor cells from pleural and ascites, and the cultivation of 3D tumor analogs

CN104962520 A discloses a method for separating and purifying tumor cells from malignant pleural effusion, but does not involve the culture method of pleural effusion tumor cells; CN106190980 A discloses a method for culturing esophageal cancer tumor cells in vitro based on human esophageal cancer tissue and Its special medium, which still uses ordinary in vitro medium and some cytokines artificially added, cannot reflect the internal environment well, affects the accuracy and authenticity of the detection data, and has a certain gap in achieving the purpose of clinical treatment

In summary, in the prior art, there is still a lack of methods for isolating tumor cells from body fluids such as pleural effusion, ascites, and cerebrospinal fluid of tumor patients, and cultivating tumor analogues under 3D conditions using a medium that can simulate the in vivo environment of tumor cells

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