Kiwi berry seed initiator, and initiation method
A technology of kiwifruit and initiator, which is applied in the fields of seed and rhizome treatment, botanical equipment and methods, biocides, etc., can solve the problems of undiscovered kiwifruit seed initiator and initiation method, unfavorable production cost, long treatment time, etc., and achieve Improve the germination rate and germination uniformity, save production costs, and the effect of simple method
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Embodiment 1
[0027] The original source of Aspergillus niger used in this example was purchased from the standard strain of the China General Microorganism Culture Collection and Management Center, and its preservation number is CGMCC 3.3928, which meets the National Military Standard GJB 150.10A-2009 of the People's Republic of China and the National Military Standard of the People's Republic of China GJB 4.10-83.
[0028] All other reagents used were of analytical grade.
[0029] The priming solution for kiwifruit seeds was prepared as follows:
[0030] 1. Activate Aspergillus niger with the preservation number CGMCC 3.3928, inoculate it on a PDA plate, and culture it at a constant temperature of 28±2° C. for 3 days to obtain activated Aspergillus niger. Store the strains at 4°C for future use.
[0031] 2. Preparation of green tea water extract: mix fresh green tea with 95°C water according to the material-to-liquid ratio of 1:50 (g / mL), extract for 10 minutes, cool to room temperature...
Embodiment 2
[0042] The original source of Aspergillus niger used in this example was purchased from the standard strain of the China General Microorganism Culture Collection and Management Center, and its preservation number is CGMCC 3.3928, which meets the National Military Standard GJB 150.10A-2009 of the People's Republic of China and the National Military Standard of the People's Republic of China GJB 4.10-83.
[0043] All other reagents used were of analytical grade.
[0044] The priming solution for kiwifruit seeds was prepared as follows:
[0045] 1. Activate Aspergillus niger with the preservation number CGMCC 3.3928, inoculate it on a PDA plate, and culture it at a constant temperature of 28±2° C. for 5 days to obtain activated Aspergillus niger. Store the strains at 4°C for future use.
[0046] 2. Preparation of green tea water extract: mix green tea with water at 100°C according to the material-to-liquid ratio of 1:100 (g / mL), extract for 20 minutes, cool to room temperature,...
Embodiment 3
[0054] The original source of Aspergillus niger used in this example was purchased from the standard strain of the China General Microorganism Culture Collection and Management Center, and its preservation number is CGMCC 3.3928, which meets the National Military Standard GJB 150.10A-2009 of the People's Republic of China and the National Military Standard of the People's Republic of China GJB 4.10-83.
[0055] All other reagents used were of analytical grade.
[0056] The priming solution for kiwifruit seeds was prepared as follows:
[0057] 1. Activate Aspergillus niger with the preservation number CGMCC 3.3928, inoculate it on a PDA plate, and culture it at a constant temperature of 28±2° C. for 1 day to obtain activated Aspergillus niger. Store the strains at 4°C for future use.
[0058] 2. Preparation of green tea water extract: mix green tea powder with water at 80°C according to the ratio of material to liquid 1:20 (g / mL), extract for 60 minutes, cool to room temperat...
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