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Combined application of radix actinidiae chinensis total triterpenoid and radix actinidiae chinensis polysaccharide to preparation of medicines for preventing and treating digestive system tumor and lung cancer

A technology of total triterpenes and compounds, which is applied in the application field of preparing drugs for the prevention and treatment of digestive system tumors and lung cancer, can solve the problems of unclear composition, singleness, and unclear specific components, so as to improve the quality of life, increase efficiency, and improve the quality of life. The effect of tumor growth inhibitory effect

Active Publication Date: 2018-11-06
INST OF BASIC THEORY OF TCM CHINA ACADEMY OF CHINESE MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above-mentioned technology has made the components of the vine root extract relatively single by improving the extraction method, its specific components are still unclear. Although scholars at home and abroad have studied the components of the vine root extract in recent years, they have found Active ingredients such as total triterpenoids (such as ursolic acid, oleanolic acid, etc.) and vine root polysaccharides have the effect of inhibiting tumor cell proliferation, but the composition of each active ingredient in natural vine root extract is not very clear , the ratio of the identified active ingredients in the natural vine root composition is not the optimal ratio, and the growth inhibitory effect on various tumor cells still needs to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The inhibitory effect of the active ingredient composition of vine pear root on BGC-823 gastric cancer cells

[0026] 1. Cell culture

[0027] BGC-823 gastric cancer cell line was purchased from the Cell Bank of Shanghai Chinese Academy of Sciences.

[0028] (1) Cell recovery

[0029] ①Take the cryopreservation tube out of the liquid nitrogen, thaw it in a 37°C water bath, and shake it quickly to speed up the thawing of the cells. After the liquid is completely melted (about 1-1.5 minutes), spray alcohol and put it into the ultra-clean workbench.

[0030] ② Aspirate the above cell suspension into a 15ml centrifuge tube, add 1ml of culture medium into the centrifuge tube at the same time, and then put it into a centrifuge for centrifugation (800-1000rpm, 5min).

[0031] ③Discard the supernatant, then add 1ml of culture medium, shake the centrifuge tube and blow the cells with a pipette gun to suspend the cells, and then put the cell suspension into the 25cm ...

Embodiment 2

[0051] Example 2 The inhibitory effect of the active ingredient composition of vine pear root on MKN1 gastric cancer cells

[0052] The MKN1 gastric cancer cell line was purchased from the cell bank of Shanghai Chinese Academy of Sciences, and the test procedure was the same as in Example 1. The inhibition rate and IC5 of each experimental group on MKN1 gastric cancer cells 0 See Table 3 for the values.

[0053] Table 3 Inhibition rate and IC of each experimental group on MKN1 gastric cancer cells 50 value comparison

[0054] test group

Embodiment 3

[0055] Example 3 The inhibitory effect of the active ingredient composition of vine pear root on MKN7 gastric cancer cells

[0056] The MKN7 gastric cancer cell line was purchased from the Cell Bank of Shanghai Chinese Academy of Sciences, and the test procedure was the same as in Example 1. The inhibition rate and IC of each experimental group on MKN7 gastric cancer cells 50 See Table 4 for the values.

[0057] Table 4 The inhibition rate and IC of each experimental group on MKN7 gastric cancer cells 50 value comparison

[0058] test group

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Abstract

The invention discloses combined application of a radix actinidiae chinensis total triterpenoid and radix actinidiae chinensis polysaccharide to preparation of medicines for preventing and treating digestive system tumor and lung cancer. According to the preparation, the concentration of the radix actinidiae chinensis polysaccharide is 0.5mg / mL; the concentration ratio of the radix actinidiae chinensis polysaccharide to the radix actinidiae chinensis total triterpene compound is 1mg to (0.0165-0.1747)mol; the radix actinidiae chinensis total triterpenoid is prepared from ursolic acid and oleanolic acid. According to the combined application, the radix actinidiae chinensis polysaccharide is mixed with the radix actinidiae chinensis total triterpenoid according to a certain concentration ratio; compared with the single radix actinidiae chinensis polysaccharide or the single radix actinidiae chinensis total triterpenoid, the prepared compound taking the radix actinidiae chinensis as an effective component has a better effect of inhibiting tumor proliferation, so that the radix actinidiae chinensis polysaccharide and the radix actinidiae chinensis total triterpenoid can achieve a synergistic effect under a certain ratio.

Description

technical field [0001] The invention belongs to the field of antitumor drugs, and in particular relates to the application of combined use of total triterpenoids of vine root and vine root polysaccharide in the preparation of drugs for the prevention and treatment of digestive system tumors and lung cancer. Background technique [0002] The understanding of tumors in Chinese medicine can be traced back to the Yin and Zhou Dynasties, and the name of "tumor" was mentioned as early as in the oracle bone inscriptions unearthed from Yin Ruins. At the same time, as early as more than 2,000 years ago, the "Zhou Li" recorded tumors and ulcer diseases, which already included tumors and the targeted treatment methods. , treat it with five medicines, and adjust it with five flavors.” At the same time, Chinese medicine believes that the occurrence, development and changes of tumors are all the result of changes in the relationship between good and evil. On the basis of the deficiency o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K31/715A61P35/00A61K31/56A61K125/00
CPCA61K31/56A61K31/715A61K36/185A61P35/00A61K2300/00
Inventor 申力张光霁徐楚韵陈喆楼招欢张广顺
Owner INST OF BASIC THEORY OF TCM CHINA ACADEMY OF CHINESE MEDICAL SCI