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Application of the combination of total triterpenoids of vine root and vine root polysaccharide in the preparation of drugs for the prevention and treatment of digestive system tumors and lung cancer

A technology of total triterpenes and compounds, which is applied in the field of preparation of drugs for the prevention and treatment of digestive system tumors and lung cancer, can solve the problems of unclear specific components, not very clear composition, singleness, etc., achieve good tumor growth inhibitory effect, and improve survival The effect of quality and efficiency improvement

Active Publication Date: 2021-05-18
INST OF BASIC THEORY OF TCM CHINA ACADEMY OF CHINESE MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above-mentioned technology has made the components of the vine root extract relatively single by improving the extraction method, its specific components are still unclear. Although scholars at home and abroad have studied the components of the vine root extract in recent years, they have found Active ingredients such as total triterpenoids (such as ursolic acid, oleanolic acid, etc.) and vine root polysaccharides have the effect of inhibiting tumor cell proliferation, but the composition of each active ingredient in natural vine root extract is not very clear , the ratio of the identified active ingredients in the natural vine root composition is not the optimal ratio, and the growth inhibitory effect on various tumor cells still needs to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The inhibitory effect of the active ingredient composition of vine pear root on BGC-823 gastric cancer cells

[0026] 1. Cell culture

[0027] BGC-823 gastric cancer cell line was purchased from the Cell Bank of Shanghai Chinese Academy of Sciences.

[0028] (1) Cell Recovery

[0029] ①Take the cryopreservation tube out of the liquid nitrogen, thaw it in a 37°C water bath, and shake it quickly to speed up the thawing of the cells. After the liquid is completely melted (about 1-1.5 minutes), spray alcohol and put it into the ultra-clean workbench.

[0030] ② Aspirate the above cell suspension into a 15ml centrifuge tube, add 1ml of culture medium into the centrifuge tube at the same time, and then put it into a centrifuge for centrifugation (800-1000rpm, 5min).

[0031] ③Discard the supernatant, then add 1ml of culture medium, shake the centrifuge tube and blow the cells with a pipette gun to suspend the cells, and then put the cell suspension into the 25cm ...

Embodiment 2

[0051] Example 2 The inhibitory effect of the active ingredient composition of vine pear root on MKN1 gastric cancer cells

[0052] The MKN1 gastric cancer cell line was purchased from the cell bank of Shanghai Chinese Academy of Sciences, and the test procedure was the same as in Example 1. The inhibition rate and IC5 of each experimental group on MKN1 gastric cancer cells 0 See Table 3 for the values.

[0053] Table 3 Inhibition rate and IC of each experimental group on MKN1 gastric cancer cells 50 value comparison

[0054] test group Inhibition rate 1 Inhibition rate 2 Inhibition rate 3 Administration group ① 8.93％ 8.64％ 14.75％ Administration group② 13.81％ 14.41％ 14.79％ Administration group③ 18.89％ 13.60％ 19.03％ Administration group④ 66.48％ 16.40％ 23.98％ Administration group⑤ 85.41％ 71.44％ 83.05％ Dosing group IC 50 The concentration ratio of ursolic acid and oleanolic acid

Embodiment 3

[0055] Example 3 The inhibitory effect of the active ingredient composition of vine pear root on MKN7 gastric cancer cells

[0056] The MKN7 gastric cancer cell line was purchased from the Cell Bank of Shanghai Chinese Academy of Sciences, and the test procedure was the same as in Example 1. The inhibition rate and IC of each experimental group on MKN7 gastric cancer cells 50 See Table 4 for the values.

[0057] Table 4 The inhibition rate and IC of each experimental group on MKN7 gastric cancer cells 50 value comparison

[0058] test group Inhibition rate 1 Inhibition rate 2 Inhibition rate 3 Administration group ① 48.49％ 39.20％ 30.44％ Administration group② 54.52％ 39.83％ 48.31％ Administration group③ 50.71％ 48.47％ 52.19％ Administration group④ 66.44％ 65.97％ 67.16％ Administration group⑤ 69.17％ 89.83％ 96.03％ Concentration ratio of ursolic acid and oleanolic acid at IC50 of administration group 1.44 2.73 2.59 Con...

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Abstract

The invention discloses the application of the combined use of total triterpenoids of pear root and polysaccharide of pear root in the preparation of drugs for the prevention and treatment of digestive system tumors and lung cancer. In this application, the concentration of vine root polysaccharides is 0.5 mg / mL, and the concentration ratio of vine root polysaccharides and vine root total triterpenoids is 1 mg: (0.0165‑0.1747) mol; Triterpenoids consist of ursolic and oleanolic acids. In the present application, by mixing vine root polysaccharides and vine root total triterpenoids at a certain concentration ratio, the obtained vine root active ingredient composition is more effective than individual vine root polysaccharides or separate vine root total triterpenoids. Compounds have a better tumor growth inhibitory effect, and it can be seen that the polysaccharides of vine root and the total triterpenoids of vine root have a synergistic effect under a certain ratio.

Description

technical field [0001] The invention belongs to the field of antitumor drugs, and in particular relates to the application of combined use of total triterpenoids of vine root and vine root polysaccharide in the preparation of drugs for the prevention and treatment of digestive system tumors and lung cancer. Background technique [0002] The understanding of tumors in Chinese medicine can be traced back to the Yin and Zhou Dynasties, and the name of "tumor" was mentioned as early as in the oracle bone inscriptions unearthed from Yin Ruins. At the same time, as early as more than 2,000 years ago, the "Zhou Li" recorded tumors and ulcer diseases, which already included tumors and the targeted treatment methods. , treat it with five medicines, and adjust it with five flavors.” At the same time, Chinese medicine believes that the occurrence, development and changes of tumors are all the result of changes in the relationship between good and evil. On the basis of the deficiency o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61K31/715A61P35/00A61K31/56A61K125/00
CPCA61K31/56A61K31/715A61K36/185A61P35/00A61K2300/00
Inventor 申力张光霁徐楚韵陈喆楼招欢张广顺
Owner INST OF BASIC THEORY OF TCM CHINA ACADEMY OF CHINESE MEDICAL SCI