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Intra-unicellular substance detecting method based on whispering gallery mode

A technology of whispering gallery mode and detection method, applied in the field of cell lasers, can solve problems such as in vitro detection, and achieve the effects of strong practicability, good spectral line quality, and small mode volume

Inactive Publication Date: 2018-11-06
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current bioassays based on whispering gallery models are limited to in vitro assays

Method used

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  • Intra-unicellular substance detecting method based on whispering gallery mode
  • Intra-unicellular substance detecting method based on whispering gallery mode
  • Intra-unicellular substance detecting method based on whispering gallery mode

Examples

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Effect test

Embodiment example 1

[0021] The laser light source used in this experiment is 473nm, or pulsed lasers of other bands (the wavelength of the laser is determined by the excitation wavelength of the fluorescent substance used), and the detected substances are proteins, sugars, amino acids, biological enzymes, DNA and other molecules. The absorption and emission wavelength ranges of Dragon Green microspheres are as follows image 3 shown. The first thing to do is to detect the protein molecules in the solution. Add 1 μl of Dragon Green microspheres with a concentration of 10 mg / ml to the culture medium, and then add 10 μl of protein molecules with a concentration of 1 mg / ml. The Dragon Green microspheres must be first After surface sensitization treatment, such as for DragonGreen, the microspheres are suspended in MES buffer, and 1-ethyl-3-(dimethylaminopropyl) carbodiimide (EDAC) is added to make the carboxyl groups on the microspheres Activation makes it easier for protein molecules to adhere to th...

Embodiment example 2

[0024] Detect the calcium ion concentration, study the relationship between the calcium ion concentration change and the laser signal, and realize the real-time dynamic detection of the calcium ion concentration.

[0025] The dynamic balance of ions inside and outside cells is the basis for the survival of cells. As the messenger of human metabolism, calcium ions are regulated by almost all physiological activities. 2+ regulation. It is involved in various physiological functions such as nerve conduction, muscle contraction, heart contraction, brain function, enzyme function, and hormone secretion of endocrine glands. Calcium ion selective microelectrode method, radioactive tracer method, nuclear magnetic resonance method (NMR) and enzymatic analysis are suitable for the detection of free calcium in blood, serum, plasma and body fluids. Fluorescence-based flow cytometry and fluorescence imaging are often used to detect calcium ions in cells. These methods cannot reflect the...

Embodiment example 3

[0032] Label-free detection of dynamic phosphorylation modifications of intracellular protein molecules.

[0033] The human body is a complex system composed of trillions of cells. The genome controls genetic information, but most of the executors who determine the state and fate of cells are protein molecules; and the dynamic chemical modification of protein molecules, which are the main components of living organisms, has great impact on biological Ontogeny, cell fate regulation and disease development all play a decisive role. Protein phosphorylation modification is the most basic, common and important mechanism to regulate and control protein activity and function. Studying the dynamic process and mechanism of chemical modification of protein macromolecules in organisms is helpful for chemical intervention and regulation, and has important scientific significance and application value for exploring new life processes and discovering new methods of disease diagnosis and tre...

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Abstract

The invention relates to an intra-unicellular substance detecting method based on a whispering gallery mode, and belongs to the fields of laser technologies and biophotonics. The method comprises thefollowing steps: (1) whispering gallery mode microcavity selection is performed; (2) microcavity and cell are treated; (3) a concrete implementation method of detection on biological substances to betested is performed; (4) the detection ranges of the detecting method involve numerous specific biological substances such as nucleic acid, protein, cells and biomolecules. The detection sensitivity is high; the detection can be realized in cells. By the whispering gallery mode, fluorescence can form resonant vibration in the cavity; laser is generated; signals are greatly enhanced; the tracking detection time is prolonged.

Description

technical field [0001] The invention relates to a cell laser, which is a technique for detecting substances in cells by using a whispering gallery mode microcavity, and belongs to the fields of laser technology and biophotonics. Background technique [0002] As a special optical resonant cavity, the whispering gallery mode optical microcavity localizes photons in the microcavity for a long time to form a whispering gallery mode dielectric resonant cavity through continuous total reflection at the boundary. Due to its unique whispering gallery mode, it has superior characteristics such as ultra-high Q value, extremely small mode volume, ultra-high energy density and extremely narrow linewidth, thus becoming the most typical type of photonic device. [0003] Optical microcavities are widely used in many fields, especially for biosensing and detection. When light is totally reflected on the surface of the microcavity, an evanescent wave (approximately in the range of ~200nm) i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6402G01N21/645G01N21/6486G01N2021/6417G01N2021/6469
Inventor 王秀红王亚平
Owner BEIJING UNIV OF TECH
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