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Antimicrobial composition

An antibacterial composition and a technology for antibacterial effect, applied in the field of antibacterial compositions, can solve the problems of not showing the proliferation inhibition effect of MRSA, Pseudomonas aeruginosa or non-tuberculous mycobacteria, and achieve the cure of respiratory tract infection, infection prevention and symptom improvement. Effect

Inactive Publication Date: 2018-11-09
NAT UNIV CORP TOKYO MEDICAL & DENTAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, Patent Document 1 only shows a growth inhibitory effect on influenza virus (specifically, mouse influenza virus PR8 [A / PR / 8 / 34(H1N1)]), and does not show any effect on MRSA, Pseudomonas aeruginosa, or non-tuberculous Proliferation inhibitory effect of mycobacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Proliferation inhibitory effect of lysozyme-chitosan complex, lysozyme alone, chitosan alone and mixture of lysozyme and chitosan on MRSA in diluted medium

[0048] In this test, MRSA IID1677 was used and the strain was cultured at 37° C. for 20 hours in a plain broth medium. The cultured bacterial solution was recovered by centrifugation (3000 rpm, 10 minutes), and suspended in sterilized water. Then, it was diluted with sterilized water so that the absorbance OD600=1.0. Dilute it 2000 times (equivalent to 10 6 CFU / ml), as the reaction bacteria solution.

[0049] 100 μl of the reaction bacteria liquid was added to each LB test medium sample, and shaking culture was performed at 37° C. for 48 hours. Here, each LB test medium sample was prepared by adding the following substances to 10 ml of 1 / 2.5 LB medium.

[0050] (1) 10ml of sterilized water,

[0051] (2) 10 ml of lysozyme-chitosan complex with a concentration of 0.002% (concentration after addition: 0.001%),

...

Embodiment 2

[0059] Inhibitory effect of lysozyme-chitosan complex, lysozyme alone, chitosan alone and mixture of lysozyme and chitosan on the proliferation of Pseudomonas aeruginosa in diluted medium

[0060] In this test, Pseudomonas aeruginosa NBRC13275 was used, and the strain was cultured at 37° C. for 20 hours using a common broth medium. The cultured bacterial solution was recovered by centrifugation (3000 rpm, 10 minutes), and suspended in sterilized water. Then, it was diluted with sterilized water so that the absorbance OD600=1.0. Dilute it 2000 times (equivalent to 10 6 CFU / ml), as the reaction bacteria solution.

[0061] 100 μl of the reaction bacteria liquid was added to each TSB test medium sample, and shake cultured at 37° C. for 48 hours. Here, each TSB test medium sample was prepared by adding 200 μl of 1M phosphate buffer (pH 7.0) to 10 ml of 1 / 2.5 TSB medium, and further adding the following substances.

[0062] (1) 10ml of sterilized water,

[0063] (2) 10 ml of ly...

Embodiment 3

[0070] Proliferation inhibitory effect of lysozyme-chitosan complex, lysozyme alone, chitosan alone and mixture of lysozyme and chitosan on lung MAC in diluted medium

[0071] In this experiment, Mycobacterium avium Chester JCM15429 was used and the strain was cultured at 37° C. for 2 weeks using Middlebrook 7H9 medium. The cultured bacterial solution was recovered by centrifugation (3000 rpm, 10 minutes), and suspended in sterilized water. Then, it was diluted with sterilized water so that the absorbance OD600=1.0. Dilute it 2000 times (equivalent to 10 6 CFU / ml), as the reaction bacteria solution.

[0072] 100 μl of reaction bacteria solution was added to each Middlebrook 7H9 test medium sample, and cultured statically at 37° C. Here, each Middlebrook 7H9 test medium sample was prepared by adding the following substances to 10 ml of 1 / 2.5 Middlebrook 7H9 medium.

[0073] (1) 10ml of sterilized water,

[0074] (2) 10 ml of lysozyme-chitosan complex with a concentration o...

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Abstract

Provided is a novel antimicrobial composition, which is against an infectious microorganism causing a respiratory infection, using natural food additives with high safety. The antimicrobial composition comprises a complex, in which lysozyme is bound to chitosan, and has an antimicrobial effect against an infectious microorganism inducing a respiratory infection.

Description

technical field [0001] The present invention relates to an antibacterial composition having an antibacterial effect on infectious bacteria causing respiratory tract infection. Background technique [0002] Nontuberculous mycobacteria such as MRSA, Pseudomonas aeruginosa, or MAC (Mycobacterium avium complex) are infectious bacteria that cause respiratory tract infections. This infectious disease is considered a problem because it is difficult to treat. [0003] Here, as a prophylactic / therapeutic agent for respiratory tract infection, as shown in Patent Document 1, it is conceivable to contain lactic acid bacteria belonging to Lactobacillus acidophilus as an active ingredient with an anti-respiratory tract infection effect. [0004] However, Patent Document 1 only shows a growth inhibitory effect on influenza virus (specifically, mouse influenza virus PR8 [A / PR / 8 / 34(H1N1)]), and does not show any effect on MRSA, Pseudomonas aeruginosa, or non-tuberculous Proliferation inhib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/46A01N43/16A01N63/00A01P3/00A61K31/722A61P31/04
CPCA01N37/46A61K38/47C12Y302/01017A61K47/61A61P11/00A61P31/04A61P43/00A01N25/32A01N43/16A01N25/10
Inventor 宫崎泰成影岛宏纪齐藤章
Owner NAT UNIV CORP TOKYO MEDICAL & DENTAL UNIV
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