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Methods for dynamic functionalization of cell membranes

A dynamic function, cell membrane technology, applied in the field of biochemistry, to achieve high cytocompatibility, avoid long-term effects, and broad application prospects

Active Publication Date: 2021-09-03
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(AccountsChem Res.2016; 49:792-800) Cell membrane macromolecular engineering can also play a similar protective role, however, few studies have specifically designed how to disintegrate

Method used

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  • Methods for dynamic functionalization of cell membranes
  • Methods for dynamic functionalization of cell membranes
  • Methods for dynamic functionalization of cell membranes

Examples

Experimental program
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Effect test

Embodiment 1

[0033] 1. Add a certain amount of SDS to the bovine serum containing 1 free sulfhydryl group and 17 disulfide bonds to promote the exposure of the hydrophobic region of the protein, and then add an appropriate amount of DTT to break the disulfide bonds to obtain albumin containing free sulfhydryl groups .

[0034] 2. Interact an appropriate amount of activated protein with the target cell-B16 cells for 20 minutes to several hours.

[0035] 3. Centrifuge to remove excess material, and after washing several times, obtain cell membrane engineered cells.

[0036] Scanning electron microscope pictures before and after B16 cells engineered with bovine serum albumin used in this example are as follows: figure 1 As shown, it can be clearly seen that the cell membrane of B16 cells has obvious changes.

[0037] 4. Proliferation ability analysis: After CFSE marks the cell membrane, when the cells divide and proliferate, the fluorescent cytoplasmic protein is evenly distributed to the s...

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Abstract

The invention relates to a method for dynamic functionalization of cell membranes. A disulfide substance with high reactivity undergoes an exchange reaction with sulfhydryl groups on the surface of cell membranes in a physiological environment to perform dynamic functionalization on cell membranes and obtain cells engineered with cell membrane materials. Compared with the prior art, the process of functionalizing the cell membrane in the present invention is applicable to different cell types, the whole process has high cytocompatibility, and the disulfide substance with high reactivity used can be degraded by itself after a period of time, Effectively avoid long-term effects on cell function. This technology can temporarily regulate cell functions such as proliferation, cycle, and endocytosis efficiency, and has broad application prospects in cell fate and function regulation.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for dynamic functionalization of cell membranes. Background technique [0002] As the boundary of the cell structure, the cell membrane is composed of phospholipid bilayers and mosaic proteins. It is not only a natural barrier to maintain the relatively independent environment between the cell and the surrounding environment, but also an intelligent gateway to ensure the exchange of complex substances, energy, and information between the cell and the surrounding environment. The cell membrane itself is an incomparably complex, precise, dynamic, ordered and disordered molecular engineering. The dynamics of cell membrane structure and biological functional characteristics pose challenges for cell membrane macromolecular engineering, which are common key technical issues. Since the cell structure is always in dynamic changes, insufficient structure can eas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00
CPCC12N5/00
Inventor 李永勇董海青何良华李艳
Owner TONGJI UNIV
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