Detection method for cattle RET (Reticulocyte) gene single nucleotide polymorphism site and detection kit and application thereof

Abstract

The invention discloses a detection method for cattle RET (Reticulocyte) gene single nucleotide polymorphism sites. The detection method comprises the following steps: by taking whole genome DNA (Deoxyribonucleic Acid) of cattle to be detected with an RET gene as a template, a primer group P as a primer, carrying out PCR (Polymerase Chain Reaction) amplification on the cattle RET gene; digesting aPCR amplification product with a restriction enzyme MaeI (XspI), and carrying out native polyacrylamide gel electrophoresis on segments after digestion; according to polyacrylamide gel electrophoresis results, together with sequencing, judging the genotype of a single nucleotide polymorphism site in a 34690 position of the cattle RET gene. The invention further discloses application of the detection kit for cattle RET gene single nucleotide polymorphism sites in molecular breeding of cattle. Because of significant biological functions of the RET gene, the single nucleotide polymorphism of thegene has remarkable influence upon dip lengths, heights and chest circumferences of cattle at early stages and is applicable to auxiliary selection on molecular markers for meat and growth propertiesof the cattle, and furthermore cattle groups with excellent genetic resources can be rapidly established.

Description

technical field [0001] The invention belongs to the technical field of biogenetic engineering, and in particular relates to a method for detecting a single nucleotide polymorphism site of a cattle RET gene, a detection kit and an application thereof. Background technique [0002] Cattle are one of the main livestock resources in my country, but now they are facing the pressure of insufficient provenance of excellent cattle breeds and poor genetic quality of the population. Traditional breeding methods are based on determining the phenotype of animals and using the genetic information of their parents, ancestors and other relatives in animal models to achieve genetic evaluation. Generally, it is assumed that traits are affected by genetic differences of many genes, so each gene is considered There are relatively small contributions to the trait, with the result that the estimates of the trait are inevitably somewhat biased. Molecular genetic markers are one of the fastest-gr...

AI Technical Summary

Problems solved by technology

However, in the selection of breeding stock, the recessive pathogenic mutation cannot be directly reflected in the appearance, so people cannot screen the HSCR pathogenic mutation site

Once an individual carrying a recessive disease-causing mutation is selected as a breeding stock, its offspring will be at risk of disease, which will bring losses to the head of the household or the farm to a certain extent

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