Method for monitoring micro-ecologic maturity of extracted stool sample 16s rRNA in intestinal tract of infant

Abstract

The invention discloses a method for monitoring micro-ecologic maturity of extracted stool sample 16s rRNA in intestinal tract of the infant. The method comprises the processes of sample collecting, 16s-rRNA gene sequencing, sequencing data analyzing, intestinal tract microorganism maturity and intestinal tract cell age computing, and use report pushing; the monitoring index of the intestinal tract microorganism maturity is proposed at the first time, the intestinal tract microorganism maturity is the similarity of the infant intestinal tract microorganism and the mother intestinal tract microorganism on the constitution, and the higher the maturity is, the better the flora development is; and the index can reflect the immunity level of the infant. Through the experimental research and thedata analysis, the infant intestinal tract microorganism evaluation can be realized through a method for detecting the hyper-variable region of the stool sample 16s rRNA V3-V4, the method is convenient for sampling, fast in analysis speed, and low in cost; the evaluation result is consistent with the existing research, and the consumption-level intestinal tract microorganism detection becomes possible.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a method for monitoring the maturity of intestinal microecology of infants by extracting 16s rRNA from stool samples. Background technique [0002] Human intestinal microbes colonize the intestinal tract, the number of which is more than 10 times that of the human body's own cells, and it plays an extremely important role in the metabolism of nutrients, the development of the human body, immunity and the occurrence of diseases. Research on intestinal microbes in infants has found that the composition of early intestinal microbes is crucial to the development of intestinal microbes and personal health in later stages, and the destruction of intestinal microbes in infants and young children will lead to asthma, diabetes, autism, asthma, etc. disease arises. In addition, many studies have reported that the intestinal microbes of infants show non-random changes with age. By u...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for monitoring the maturity of intestinal microecology of infants and young children by extracting 16s rRNA from feces samples. Composition of intestinal flora, calculation of intestinal microbial maturity, in order to solve the above background technology proposed to detect 16s rRNA in feces samples by high-throughput next-generation sequencing at room temperature, analyze the composition of intestinal flora of infants, and Continuously monitor the maturity of intestinal microecology to assess the development status of infants and young children's intestinal microbiome and the status of body immunity

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