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A method for identifying whether a Nvjin pill contains cynanchum komarovii being an adulterant of blackend swallowwort root

A technology of Laoguatou and Nvjinwan, which is applied in the field of identification of whether Nvjinwan contains Baiwei counterfeit Laoguatou, can solve the problems of doping Laoguatou and being unable to determine whether Baiwei is present, and achieves simple steps and easy judgment , clear results

Active Publication Date: 2018-11-20
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problems in the prior art that it is impossible to determine whether Baiwei is mixed with old melon heads during the production process of Nvjin Wan preparations, this application provides a method for identifying whether Nvjin Pills contain fake Baiwei old melon heads

Method used

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  • A method for identifying whether a Nvjin pill contains cynanchum komarovii being an adulterant of blackend swallowwort root
  • A method for identifying whether a Nvjin pill contains cynanchum komarovii being an adulterant of blackend swallowwort root
  • A method for identifying whether a Nvjin pill contains cynanchum komarovii being an adulterant of blackend swallowwort root

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1 Instruments and reagents

[0054] Instrument: Sartorius CP225D electronic balance; Shimadzu LC-20A high performance liquid chromatography (diode array detector); reagents: methanol is chromatographically pure, water is purified water prepared by Millipore, and other reagents are analytically pure.

[0055] 2 Chromatographic conditions

[0056] 2.1 Preparation of reference solution

[0057] Take about 0.4g of old melon head powder, add 30ml of methanol, ultrasonic for 30min, filter, evaporate to dryness, add 2ml of methanol to the residue to dissolve, filter through a 0.45μm microporous membrane, and purify by semi-preparative HPLC, the chromatographic column is octadecane Based on silane-bonded silica gel, the mobile phase is methanol-0.1% phosphoric acid solution, the volume ratio is 30:70, after detecting all the eluents at 325nm, it is determined to collect the eluents with a retention time of 15.7-15.9 minutes, and concentrate under reduced pressure dry.

[005...

Embodiment 2

[0064] Embodiment 2: methodological investigation

[0065] Study on the specificity of the characteristic components of Laoguatou (ie reference substance)

[0066] According to the conditions of high performance liquid chromatography under 2.2., the other 22 medicinal materials in Nvjin Wan preparations were investigated, and the results showed that no characteristic components of Laoguatou were detected in these 22 medicinal materials, indicating that this method can be used for exploration and analysis of Nvjin Wan. Quality inspection of Baiwei in Jinwan preparation. The HPLC chromatograms of 22 herbs are shown in Figure 1-25 .

[0067] According to the HPLC chromatograms of 22 medicinal materials, it is feasible to use the reference substance as a characteristic component to identify whether Baiwei in the Nvjin Wan preparation is mixed with Laoguatou.

Embodiment 3

[0068] Embodiment 3: sample determination

[0069] 96 batches of Nvjin Wan preparations collected are tested, the method is as in Example 1, and the test results are as follows:

[0070] Accurately draw 10 μL each of the reference substance solution and the above-mentioned test solution, and inject them into the liquid chromatograph for determination. Chromatogram see Figures 26-28 .

[0071] After determination, among the 96 production batches of Nvjin Wan preparations collected, chromatographic peaks with the same retention time as that of the reference product appeared in the chromatograms of 52 batches of samples, and the UV- The absorption spectrum can be seen, and the absorption spectrum is the same, and there is a situation where old melon heads are used instead of Bai Wei for feeding. Problem samples accounted for 54.2% of all samples. The established method is accurate and reliable. The identification method disclosed by the invention will play a huge role in st...

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Abstract

The invention relates to a method for identifying whether a Nvjin pill contains cynanchum komarovii which is an adulterant of blackend swallowwort root in the technical field of drug analysis. The method includes separately injecting a to-be-detected sample and a reference substance into a liquid chromatograph to perform detection; taking a reference substance spectrum to perform positioning; andjudging whether the to-be-detected sample contains cynanchum komarovii. The method successfully solves detection methods in the prior art that whether the cynanchum komarovii is fed instead of blackend swallowwort root during Nvjin pill preparation production, so that the method can play a huge role to standardize the feeding of the blackend swallowwort root in Nvjin pill preparation; and the method is simple in operation and steps, clear in results and easy to judge.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for identifying whether or not Nujin Pills contain fake Baiwei Laoguatou. Background technique [0002] The prescription of Nvjin Wan is composed of 23 medicinal materials such as Angelica, Radix Paeoniae Alba, Chuanxiong and Baiwei. Among them, Baiwei is a plant of the family Radix chinensis Baiwei ( Cynanchum atratum Bge.) or creeping white myrtle ( Cynanchum versicolor Bge.) dry roots and rhizomes. Through research on the medicinal material market, it was found that most of the Baiwei sold in the market is the old melon head of the Radixaceae plant ( Cynanchum komarovii The dried roots and rhizomes of Al. Iljinski (referred to as Laoguatou) have different sources, that is, Laoguatou is a confusing product of Baiwei. [0003] Although there are differences in the shape of Baiwei and Laoguatou medicinal materials, Baiwei in the market is mostly circulate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 穆向荣林林郭东晓林永强徐丽华
Owner SHANDONG INST FOR FOOD & DRUG CONTROL