Application of chitosan-Se in inhibiting reduction of ERbeta gene expression of epithelial cells of porcine endometrium caused by F-2 toxins

A technology of chitosan-selenium and endometrium, which is applied in the field of cell engineering, can solve the problems of reducing, not seeing chitosan-selenium, and not seeing the expression of ERβ gene in porcine endometrial epithelial cells

Inactive Publication Date: 2018-11-23
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no patents or reports on chitosan-selenium in alleviating the toxic effect of F-2 toxin on endometrial epithelial cells at home and abroad, let alone chitosan-selenium inhibiting F-2 toxin-induced pig endometrium Patents or reports on the reduction of ERβ gene expression in epithelial cells

Method used

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  • Application of chitosan-Se in inhibiting reduction of ERbeta gene expression of epithelial cells of porcine endometrium caused by F-2 toxins
  • Application of chitosan-Se in inhibiting reduction of ERbeta gene expression of epithelial cells of porcine endometrium caused by F-2 toxins
  • Application of chitosan-Se in inhibiting reduction of ERbeta gene expression of epithelial cells of porcine endometrium caused by F-2 toxins

Examples

Experimental program
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Effect test

Embodiment 1

[0020] 1.1 Isolation and purification of porcine endometrial cells

[0021] (1) Isolation of endometrial cells

[0022] Immediately after the slaughter of the pig, the two ends of the uterus were ligated, and the whole uterus was aseptically removed, the fat tissue was peeled off, rinsed with 200 IU double-antibody pre-cooled normal saline solution, placed in the double-antibody-containing normal saline solution, and sent to the hospital as soon as possible. to the lab. Clamp both ends of the uterus with hemostatic forceps, take out the uterus in the ultra-clean workbench, soak it in alcohol for about 60 seconds, wash it with PBS after taking it out; cut the uterus longitudinally (close to the uterine horn), wash it 3-4 times with double-antibody PBS, without Bloodstain, cut endometrium to 1 mm 3 Left and right granular, placed in the cell culture plate evenly at intervals of about 0.5 cm, after the tissue block was attached to the cell culture plate, add a small amount of c...

Embodiment 2

[0056] 2.1 Isolation and purification of porcine endometrial cells

[0057] (1) Isolation of endometrial cells

[0058] Immediately after the slaughter of the pig, the two ends of the uterus were ligated, and the whole uterus was aseptically removed, the fat tissue was peeled off, rinsed with 200 IU double-antibody pre-cooled normal saline solution, placed in the double-antibody-containing normal saline solution, and sent to the hospital as soon as possible. to the laboratory. Clamp both ends of the uterus with hemostatic forceps, take out the uterus in the ultra-clean workbench, soak it in alcohol for about 60 seconds, wash it with PBS after taking it out; cut the uterus longitudinally (close to the uterine horn), wash it 3-4 times with double-antibody PBS, without Bloodstain, cut endometrium to 1 mm 3 Left and right granular, placed in the cell culture plate evenly at intervals of about 0.5 cm, after the tissue block was attached to the cell culture plate, add a small amou...

Embodiment 3

[0092] 3.1 Isolation and purification of porcine endometrial cells

[0093] (1) Isolation of endometrial cells

[0094] Immediately after the slaughter of the pig, the two ends of the uterus were ligated, and the whole uterus was aseptically removed, the fat tissue was peeled off, rinsed with 200 IU double-antibody pre-cooled normal saline solution, placed in the double-antibody-containing normal saline solution, and sent to the hospital as soon as possible. to the lab. Clamp both ends of the uterus with hemostatic forceps, take out the uterus in the ultra-clean workbench, soak it in alcohol for about 60 seconds, wash it with PBS after taking it out; cut the uterus longitudinally (close to the uterine horn), wash it 3-4 times with double-antibody PBS, without Bloodstain, cut endometrium to 1 mm 3 Left and right granular, placed in the cell culture plate evenly at intervals of about 0.5 cm, after the tissue block was attached to the cell culture plate, add a small amount of c...

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Abstract

The invention discloses application of chitosan-Se in inhibiting the reduction of ERbeta gene expression of epithelial cells of porcine endometrium caused by F-2 toxins. Experiment results show that the reduction of the ERbeta gene expression of the epithelial cells of the porcine endometrium caused by the F-2 toxins can be inhibited by 0.8-3.0 mu mol / L chitosan-Se (final concentration of Se in aculture solution) when the concentration of the F-2 toxin is within 10-30 mu g / mL, so that the toxic effect, generated by the F-2 toxins, on the epithelial cells of the porcine endometrium cultured invitro is weakened, and an in vitro research model and theoretical support are provided for application of chitosan-Se in prevention and treatment of F-2 toxin poisoning of pigs.

Description

technical field [0001] The invention relates to the cell engineering technology in the field of biotechnology, in particular to the application of chitosan selenium in inhibiting the reduction of ERβ gene expression of porcine endometrium epithelial cells caused by F-2 toxin. Background technique [0002] F-2 toxin, also known as zearalenone, is a mycotoxin produced by the genus Fusarium. It is a dihydroxybenzoic acid lactone compound. It has a similar structure to estradiol and has estrogen-like activity. It is widely present in molds. It is one of the main mycotoxins in food and feed contamination. A large number of studies at home and abroad have proved that F-2 toxin has reproductive toxicity, cytotoxicity, liver and kidney toxicity, immunotoxicity and genotoxicity. Long-term feeding of feed contaminated with F-2 toxin will lead to decreased growth performance, low immune function and reproductive dysfunction of livestock and poultry. [0003] F-2 toxin is similar in s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12Q1/6851A61K33/04A61P15/00A61K31/722
CPCA61K31/722A61K33/04A61P15/00C12N5/0682C12Q1/6851C12Q2531/113C12Q2545/101A61K2300/00
Inventor 秦顺义王欢欢张建斌马吉飞杨升李留安赵瑞利丁向彬
Owner TIANJIN AGRICULTURE COLLEGE
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