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Method for obtaining stevioside RA from chrysanthemum morifolium by using stevia rebaudiana extract

A technology of steviol glycoside and stevia leaf, applied in the field of cell biology and biology, can solve the problems of increased steviol glycoside RA production, low steviol glycoside RA content and the like

Inactive Publication Date: 2018-11-30
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

However, currently the content of steviol glycoside RA in stevia leaves is relatively low. If steviol glycoside RA can be enriched in chrysanthemums of the same family, and the biomass of chrysanthemum plants is larger than that of stevia plants, the output of stevioside RA will increase. greatly increase

Method used

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  • Method for obtaining stevioside RA from chrysanthemum morifolium by using stevia rebaudiana extract
  • Method for obtaining stevioside RA from chrysanthemum morifolium by using stevia rebaudiana extract
  • Method for obtaining stevioside RA from chrysanthemum morifolium by using stevia rebaudiana extract

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Embodiment Construction

[0033] Solution of the present invention:

[0034] 1. Obtain stevioside crude extract

[0035] The leaves on the plants of the Stevia rebaudiana variety 'Runnong' were picked, put into a drying oven to dry, the temperature of the drying oven was set at 55°C, and dried for 48 hours before use.

[0036] After crushing 500g of dried stevia leaves, pour them into an 80-mesh sieve to filter, weigh 50g of the filtered powder in a beaker, add 230ml of purified water and 5ml of 2% cellulase, and control the pH at about 5, ℃ water bath in a water bath for 90 minutes, filter with a Buchner funnel, after centrifugation, take the supernatant and filter. After further adding 230ml of purified water and 5ml of 2% cellulase to the residue taken out, heat in a water bath for 45min, filter with suction, and take the filtrate. Then, after adding 5g of calcium oxide and 4g of aluminum potassium sulfate to the filtrate, remove impurities in a water bath at 50°C for 40 minutes, stir and filter i...

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Abstract

The invention relates to a method for increasing content of RA in leaves of stevia rebaudiana through in-vitro culture of the stevia rebaudiana and belongs to the fields of cytobiology and biology. Astevia rebaudiana leaf extract is sprayed to chrysanthemum morifolium 'Taishan Mountain bright pearl' plants, so that 'Taishan Mountain bright pearl' leaves contain a stevioside RA ingredient; and byusing the characteristics such as high resistance and large biomass of the chrysanthemum morifolium, entire plants of 'Taishan Mountain bright pearl' contain the stevioside RA ingredient, and thus, the chrysanthemum morifolium for tea becomes sweeter. The method comprises the steps of obtaining a stevioside crude extract, spraying the stevioside crude extract to chrysanthemum morifolium plants, extracting the stevioside from the chrysanthemum morifolium leaves by a cellulose enzyme method, purifying the stevioside crude extract by a macroporous resin adsorption method, drying the stevioside extract in the chrysanthemum morifolium by evaporation by a rotary evaporator method, and Analyzing the content of the stevioside in the leaves of the chrysanthemum morifolium by a high performance liquid chromatograph.

Description

technical field [0001] The invention relates to a method for in vitro culture of Stevia rebaudiana Bertoni to increase RA content in leaves of Stevia rebaudiana, belonging to the field of cell biology and biology. Background technique [0002] Stevia is a herbaceous plant of the genus Stevia (Stevia) in the family Compositae. It is native to Paraguay in South America. Its leaves contain stevioside (stevioside, ST), which is 200-300 times sweeter than sucrose. Low-sweetness energy-type sweet substances. It has been proved by modern science that stevia has a good preventive effect on patients with hypertension, diabetes, arteriosclerosis and coronary heart disease. Drinking stevia tea can help relieve mental fatigue, promote metabolism, and benefit human health. At present, studies have shown that steviol glycosides include rebaudioside A (Rebaudioside A, RA), RB, RC, RD, RA and other compounds. Among them, stevioside (ST) and rebaudioside A (RA) account for the largest pro...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H15/256G01N30/06
CPCC07H15/256C07H1/08G01N30/06
Inventor 郑成淑于璐耿希
Owner SHANDONG AGRICULTURAL UNIVERSITY
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