Unlock instant, AI-driven research and patent intelligence for your innovation.

Extracting method of corn protein peptide

A technology of corn protein peptide and extraction method, applied in the field of corn protein peptide extraction, can solve the problems of long cycle, affecting the growth and development of offspring, and loss of activity of corn protein

Inactive Publication Date: 2018-11-30
ANHUI SHUNXIN SHENGYUAN BIOLOGICAL FOOD CO LTD
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its complex heredity and rich variety of variations, there are shortcomings in the conventional breeding process, such as too long cycle, too large coefficient of variation, and affecting the growth and development of offspring. Modern biological breeding technology not only overcomes the above-mentioned shortcomings and deficiencies, It also improves breeding speed and quality
[0003] After retrieval, the patent number is CN201310457453.2, which proposes a method for extracting protein powder from corn germ meal. In this patent, pasteurization at 85°C is used. High temperature is easy to make corn protein lose its activity at the beginning, thus affecting the quality of the product. , for this reason, the present invention proposes a kind of extraction method of zein peptide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The extraction method of a kind of zein peptide that the present invention proposes, comprises the following steps,

[0023] S1, degreasing corn germ to obtain corn germ meal, putting the corn germ meal into a pulverizer to crush, passing through an 80-mesh sieve, adding water and stirring evenly, and preparing corn germ meal with a protein concentration of 8% (w / w) The mixed solution is pasteurized to obtain the sterilized corn germ meal mixed solution A;

[0024] S2, adjusting the pH of A in S1 to 7.8 with 0.09mol / l NaOH solution;

[0025] S3, adding compound protease, the addition amount is 0.05% of the weight of the corn germ meal mixed liquid, stirring slowly at 30°C, stirring at a speed of 15 rpm, maintaining for 2 hours, and performing enzymatic hydrolysis to obtain enzymatic hydrolysis solution B;

[0026] Add alkaline protease Promod523P to the enzymolysis solution B obtained in S4 and S3, and add 1.25 μL per 10 g of the enzymolysis solution. At this time, the...

Embodiment 2

[0031] The extraction method of a kind of zein peptide that the present invention proposes, comprises the following steps,

[0032] S1, degreasing corn germ to obtain corn germ meal, put the corn germ meal into a pulverizer to crush, pass through a 80-100 mesh sieve after crushing, add water and stir evenly, and prepare corn with a protein concentration of 12% (w / w) The germ meal mixed solution is pasteurized to obtain the sterilized corn germ meal mixed solution A;

[0033] S2, adjusting the pH of A in S1 to 8.2 with 0.09mol / l NaOH solution;

[0034] S3, adding compound protease, the addition amount is 0.05% of the weight of the corn germ meal mixed solution, stirring slowly at 50°C while keeping the temperature at 22 rpm for 2 hours, and performing enzymatic hydrolysis to obtain enzymatic hydrolysis solution B;

[0035] Add alkaline protease Promod523P to the enzymolysis solution B obtained in S4 and S3, and add 1.25 μL per 10 g of the enzymolysis solution. At this time, th...

Embodiment 3

[0040] The extraction method of a kind of zein peptide that the present invention proposes, comprises the following steps,

[0041] S1, degreasing corn germ to obtain corn germ meal, putting the corn germ meal into a pulverizer to crush, passing through a 90-mesh sieve, adding water and stirring evenly, and preparing corn germ meal with a protein concentration of 10% (w / w) The mixed solution is pasteurized to obtain the sterilized corn germ meal mixed solution A;

[0042] S2, adjusting the pH of A in S1 to 8.0 with 0.09mol / l NaOH solution;

[0043] S3, adding compound protease, the addition amount is 0.05% of the weight of the corn germ meal mixed solution, stirring slowly at 40°C while keeping the temperature at 18 rpm for 2 hours, and performing enzymatic hydrolysis to obtain enzymatic hydrolysis solution B;

[0044] Add alkaline protease Promod523P to the enzymolysis solution B obtained in S4 and S3, and add 1.25 μL per 10 g of the enzymolysis solution. At this time, the p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an extracting method of corn protein peptide. The extracting method comprises the following steps: degreasing corn germs so as to obtain corn germ meal; crushing the corn germmeal in a pulverizer; filtering the crushed corn germ meal by virtue of a sieve of 80-100 meshes, so as to prepare a corn germ meal mixed solution that protein concentration is 8-12% (w / w); adding analkaline protease Promod523P solution by an amount of 1.0-1.5[mu]L in terms of 10g of an enzymatic hydrolysate, wherein pH value of the enzymatic hydrolysate is 8-9; controlling a constant water-bathtemperature at 30-55 DEG C for 40min so as to dissolve protein, so that a protein dissolving solution is formed; and then, adding neutral protease to the protein dissolving solution by an amount of 0.7-1.5[mu]L in terms of 10g of the protein dissolving solution, wherein pH value is 4.2-5.2; and continuing to keep temperature at 35-45 DEG C for 15min. With the application of the extracting method provided by the invention, quality and activity of the finished product (the corn protein peptide) can be improved, so that the finished product is safer and more healthy; and the extracting method ismature in extracting process and suitable for promotion.

Description

technical field [0001] The invention relates to the technical field of corn protein peptide extraction, in particular to a method for extracting corn protein peptide. Background technique [0002] Maize is an annual monoecious cross-pollinated plant with tall plants and strong stems. It is an important food crop and feed crop. It is also the crop with the highest total output in the world. Its planting area and total output are second only to rice and wheat. Corn has always been known as a longevity food, rich in protein, fat, vitamins, trace elements, cellulose, etc., and has great potential for developing high-nutrition, high-biological function foods. However, due to its complex heredity and rich variety of variations, there are shortcomings in the conventional breeding process, such as too long cycle, too large coefficient of variation, and affecting the growth and development of offspring. Modern biological breeding technology not only overcomes the above-mentioned shor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 张榆敏
Owner ANHUI SHUNXIN SHENGYUAN BIOLOGICAL FOOD CO LTD