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Application of Acetaldehyde in Preparation of Nematode Insecticide

A nematode and acetaldehyde technology, applied in the field of agricultural microbiology, can solve the problems of human and livestock insecurity, few root-knot nematodes, and restrictions on agronomic traits, and achieve sustainable results

Inactive Publication Date: 2020-10-16
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its toxic residues, chemical nematicides seriously pollute the environment and are not safe for humans and animals during use
Many nematicides have been banned. In many developing countries, especially in tropical or subtropical regions, the climate is suitable for nematode activity and reproduction. Perennial plants and crops planted in the same field for consecutive years are often very seriously damaged by nematodes. However, developing countries need a large amount of food and must rely on perennial crops and crop continuous cropping, so crop rotation is rarely used to control root-knot nematodes; the application of resistant varieties is not widespread at present, one is due to the limitation of agronomic traits, and the other is due to After successive plantings of a resistant variety in an area, selection by the resistant variety changes the population of nematodes, increasing the number of the few nematode types to which the variety is not resistant, leading to a gradual loss of resistance

Method used

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  • Application of Acetaldehyde in Preparation of Nematode Insecticide
  • Application of Acetaldehyde in Preparation of Nematode Insecticide
  • Application of Acetaldehyde in Preparation of Nematode Insecticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The application of acetaldehyde in the preparation of root-knot nematode pesticides is as follows:

[0023] Pick nematode-infected root-knot nematodes from the tomato roots, place egg masses in a 96-well plate and incubate at 20°C to observe the hatching of root-knot nematodes; collect J2 stage larvae of root-knot nematodes in a centrifuge tube to obtain a nematode suspension; add samples to a 96-well plate 200 μl, using the solvent as a blank control, add 2 μL chloramphenicol (30 mg / mL) and 30 nematodes, each group has 3 replicates, sealed with parafilm, and placed in a constant temperature incubator; observe the experimental results at 6h and 24h.

[0024] Nematode mortality rate = number of dead nematodes / number of tested nematodes*100%

[0025] Table 1. Activity of Acetaldehyde against Meloidogyne incognita

[0026]

Embodiment 2

[0028] The application of acetaldehyde in the preparation of root-knot nematode fumigant, the application process is as follows:

[0029] Pick nematode-infected root-knot nematodes from the roots of tomatoes, place egg masses in a 96-well plate and incubate at 20°C to observe the hatching of root-knot nematodes; collect J2 stage larvae of root-knot nematodes in a centrifuge tube to obtain a nematode suspension; select one in the 96-well plate Add 200 μL of sample to the well, add 2 μL of chloramphenicol (30 mg / mL) and 60 nematodes to each well of the four wells next to the well, seal with parafilm, and place in a constant temperature incubator; observe the experimental results at 6 hours and 24 hours.

[0030] Nematode mortality rate = number of dead nematodes / number of tested nematodes*100%

[0031] Table 2. Fumigation activity of acetaldehyde against Meloidogyne incognita

[0032]

[0033] 10mg / mL acetaldehyde was used to fumigate root-knot nematodes, and the mortality r...

Embodiment 3

[0035] Inhibition of acetaldehyde on egg hatching of Meloidogyne incognita by fumigation

[0036] Pick nematode-infected root-knot nematodes from the roots of tomatoes, place egg masses in a 96-well plate and incubate at 20°C to observe the hatching of root-knot nematodes; collect J2 stage larvae of root-knot nematodes in a centrifuge tube to obtain a nematode suspension; select one in the 96-well plate Add 200 μL of sample to the well, add 1 oocyst to each well of the four wells next to the well, seal with parafilm, and place in a constant temperature incubator; observe the experimental results on 1d, 2d, and 3d.

[0037] Table 3. Inhibitory effect of acetaldehyde on egg hatching of M. incognita

[0038]

[0039] Fumigation of root-knot nematode oocysts with 10mg / mL acetaldehyde can effectively inhibit egg hatching.

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Abstract

The invention discloses a volatile substance acetaldehyde having activity on root-knot nematode. The nematicidal activity determination shows that acetaldehyde has a strong contact activity, the medium lethal concentration is EC50 / 6h (mg / L)=141.4 [mu]g / ml; the acetaldehyde has fumigating activity against root-knot nematodes; and has an inhibitory effect on hatching of root-knot nematode eggs; andchemotaxis experiments show that the acetaldehyde has an attracting effect on root-knot nematodes. The acetaldehyde is generally used as an insect attractant and a fumigant, and has insecticidal action against root-knot nematode, and inhibition effect for egg hatching and attraction effect are firstly discovered.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbiology, and in particular relates to the application of acetaldehyde in the preparation of nematode insecticides. [0002] technical background [0003] Root-knot nematodes (Meloidogyne incognita; RKNs) are exclusive root parasites that infect more than 3000 plant species worldwide and are one of the most serious plant-parasitic nematodes in the world, especially in tropical and subtropical agricultural regions. (Kong Xiangyi et al. 2006). Root-knot nematode (Meloidogyne spp.) is an endoparasitic nematode that colonizes the root system of plants, belonging to the order Tylenchida, family Heteroderidae, subfamily Meloidogyninae, genus Meloidogyne . In my country, root-knot nematode incognita is the most widely distributed and most harmful. Root-knot nematodes are sometimes difficult to control due to their wide host range, short life cycle, high reproductive rate, and endoparasitism (C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N35/02A01P5/00
CPCA01N35/02
Inventor 张吉斌邵宗泽余晨黄典蔡珉敏郑龙玉喻子牛
Owner HUAZHONG AGRI UNIV
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