A fluorescence detection device and method for an ultraviolet LED

Abstract

The present invention relates to a fluorescence detection device and method for an ultraviolet LED. The device includes a sample seat where a sample to be subjected to fluorescence detection is placed, and a sample table support used for supporting the sample seat. The sample table support is also provided with a fluorescence light source as an excitation light source and an image collector used for collecting images. The fluorescence light source is right above the sample seat. Ultraviolet light emitted by the fluorescence light source can be aligned and emitted to the sample seat. The fluorescence detection device also includes a drive power supply device used for driving the fluorescence light source, can control the light emitting frequency of the fluorescence light source, and can allow the ratio of the light emitting frequency of the fluorescence light source to the exposing frequency of the image collector to be a positive integer, thus reducing stray light in a fluorescence detection process. The fluorescence detection device is compact in structure, influences of stray light on fluorescence detection processes can be effectively reduced, the detection efficiency is increased and the device is safe and reliable.

Description

technical field [0001] The invention relates to a fluorescence detection device and method, in particular to an ultraviolet LED fluorescence detection device and method, belonging to the technical field of fluorescence detection. Background technique [0002] In the prior art, most of the early ultraviolet spectrometers used low-pressure mercury lamps as light sources, or pulsed xenon lamps with a long life, but they all had disadvantages such as pollution, large volume, high energy consumption, and severe heat generation. With the development of science and technology, LED light source with small size and low cost has been used as the excitation light source of the detector, and small molecule OLED light source is used to emit excitation light with corresponding wavelength and certain intensity for fluorescence detection. In addition, there are also UV LEDs used as excitation light sources to emit ultraviolet light to irradiate foods containing aflatoxin with a certain radi...

AI Technical Summary

Problems solved by technology

However, when UV LED is used as a fluorescent excitation source, its output power is small, and its bandwidth and color temperature are also unstable, resulting in insufficient power of UV-LED and stray light in fluorescent images.

Images