Preparation process of single nanoparticle and array-based biological molecule detector thereof

A nanoparticle and biomolecule technology, applied in the field of biomedicine, can solve the problems of expensive preparation equipment for geometric shapes, achieve high input and output characteristics, accurate sensitivity, and reduce non-isotropic adsorption.

Inactive Publication Date: 2009-11-04
宋玉军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, due to the lack of precise control of the geometry (size, shape) of individual nanoparticles or expensive preparation equipment and complex processes, especially the positioning and orientation of the individual nanoparticles prepared, how to use them in microfluidic systems It is still very challenging to prepare such biomolecular sensors by eliminating the influence of micro-fluorescence and light scattering generated by the inner wall of the microchannel on the collected optical signal during coupling, and how to eliminate non-specific adsorption during detection. This type of biosensor is still in the research stage

Method used

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  • Preparation process of single nanoparticle and array-based biological molecule detector thereof
  • Preparation process of single nanoparticle and array-based biological molecule detector thereof
  • Preparation process of single nanoparticle and array-based biological molecule detector thereof

Examples

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example 1

[0045] The multi-level microstructure prepared by ultraviolet lithography technology, and the silver nanoparticle array was prepared by nanosphere stencil etching technology in the micro-window composed of 10nm chromium layer at the last level ( Figure 4 ). Firstly, the micropatterns on the mask are transferred to the chromium layer on the glass by using ultraviolet lithography technology, and the dark field images of several micropatterns in the last stage are as follows: Figure 4 A shows that the transcribed micro-windows are regularly arranged and clearly identifiable. Then, the desired nanoparticles were prepared in specific micro-windows by using nanosphere stencil etching technique (the cross section is shown in Fig. figure 2 a), Figure 4 B is an AFM photograph of a triangular silver nanoparticle array with a side length of 350 nm prepared in a circular micropattern (R) by nanosphere (about 1000 nm in diameter) stencil method, and the right side is the three-dimens...

example 2

[0053] According to the method in Example 1, well-dispersed triangular Ag nanoparticles prepared by focused ion beam technology in a micropattern made of a 3 nm-thick indium tin oxide film on a quartz plate as the substrate. The photo of the optical CCD to determine its position in the multi-level microstructure pattern and the photo of the AFM of its size are as follows Figure 9 A and 9B are shown. Figure 9 The white dot pointed by the white dotted line in the CCD image of A is the particle, such as Figure 9 The AFM image of the particles shown in B shows that the side length of the triangular pyramidal silver nanoparticles is about 350 nm. Construct the microfluidic channel on it by the microfluidic channel preparation process and reaction process of example 1, and the nanoparticle is modified with aminododecanethiol and hydroxydecylthiol, and use the DSS coupling agent to pass through the reaction (4) and (5) The particles are functionalized with the desired bioactive ...

example 3

[0055] According to the method in example 1, in the microwindow that 2.54mm thick biaxially oriented polypropylene (BOPP) is formed by the 10 nanometer thick chromium film, prepare triangular Au nanoparticle lattice by electron beam etching technique, the particle The side length is 60 nm. AFM photos of typical nanoparticle lattices are shown in Figure 10 As shown in A, its CCD photo is shown in Figure 10 B, True color photograph of the scattered light of the nanoparticle showing that the particle emits blue-green light. Use the molding process to prepare the microfluidic channel and build the microfluidic channel on it and functionalize the particle with the desired bioactive molecule IgG, wherein the modification process replaces 12-MUA with tiopronin (C 5 h 9 NO 3 S, TP). The construction material of the micro-channel is polypropylene and copper oxide nanoparticles (the particle size range is 10-50nm, the content is 10wt%), and the capping material is 1mm BOPP. The ...

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Abstract

The invention relates to a method which is developed on the basis of the impacts of biological molecules on the single nanoparticle localized surface plasmon resonance effect and uses a localized surface plasmon resonance spectrum for detecting biological molecules, thereby solving the controllable preparation of the nanoparticle and eliminating non-specific absorption and parasitic light signalsduring the detection on the basis of positioning, orienteering, being coupled with a microfluidic system and optimizing a signal collection region in a micro-channel. The invention comprises the stepsof using the vapor deposition technology and the advanced preparation technology of micro-nano materials and structures to prepare the identifiable signal nanoparticle or a particle array in a micro-fluid, integrating the single nanoparticle or the particle array in the micro-channel, further modifying and functionalizing the surface thereof and using an optical signal generated by the impacts ofthe biological molecules on the nanoparticle localized surface plasmon resonance effect for detecting the type and the concentration of the biological molecules in the fluid. Therefore, a high-yieldsuper-sensitive chip-based biological molecule detector is constructed in the micro-channel.

Description

technical field [0001] The invention relates to the diagnosis and detection of biological preparations and biological active substances, belonging to the field of biomedicine. Background technique [0002] Development of diagnostics and detection for diseases, new drug discovery, large-scale protein structure and function, and toxic organisms (bacteria, viruses, etc.) and biological agents in the environment is a very important topic. In principle, biosensors are based on signal converters that convert coupling effects between biological ligands and receptors into recognizable signals. In recent years, many signal conversion methods based on biomolecular interactions have been developed, such as optical signals, radiation signals, electrochemical signals, magnetic signals, micromechanical signals, piezoelectric effects, and mass spectrometry. The development of sequences based on large-scale miniature biomimetic signal transducers allows us to identify and track multiple bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48B82B3/00B81C1/00G01N21/25G01N21/65
CPCG01N21/554
Inventor 宋玉军
Owner 宋玉军
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