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The gene sawrky expressed by drought stress in the wild species of zebra

A technology of drought stress and wild species, applied in genetic engineering, plant genetic improvement, microbial measurement/inspection, etc., can solve the problem that WRKY gene has not been reported

Active Publication Date: 2021-10-22
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the research of sugarcane, especially the WRKY gene related to drought resistance in the wild species of P. chinensis has not been reported

Method used

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  • The gene sawrky expressed by drought stress in the wild species of zebra
  • The gene sawrky expressed by drought stress in the wild species of zebra
  • The gene sawrky expressed by drought stress in the wild species of zebra

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Acquisition of the gene SaWRKY expressed by drought stress in the wild species of Pseudomonas japonica:

[0034] 1. Design of special primers for amplifying the full-length sequence of the gene SaWRKY expressed by drought stress

[0035] According to the high-throughput transcriptome sequencing data obtained in the previous experiment, the bioinformatics analysis and screening were carried out, and the special primers for amplifying the gene SaWRKY were designed by using the biological software Primer5.0 and Oligo 7. The special primers were composed of the upstream primer GP- F and downstream primer GP-R, the base sequence of the upstream primer GP-F is shown in SEQ ID NO: 2, the base sequence of the downstream primer GP-R is shown in SEQ ID NO: 3, the Special primers were sent to Shanghai Shenggong Company for synthesis.

[0036] 2. Extraction of total RNA from the leaves of the wild species of Rhizoma chinensis treated with drought.

[0037] (1) Drought s...

Embodiment 2

[0069] Embodiment 2 A method for detecting the differential expression of the gene SaWRKY in the wild species of Prunus chinensis under drought stress provided by the present invention comprises the following steps:

[0070] 1. Drought stress treatment:

[0071] The plants of two kinds of Banmao 90-14 and Yunnan 82-53 were planted in flower pots according to the routine, and each material was divided into a control group and a treatment group, and each material in the control group and the treatment group was divided into three repeat. The control group was watered as usual, and the treatment group was subjected to drought stress for 8 days. The drought stress for 8 days was as follows: first, the same as the control group, watered as usual, and when the planted material entered the elongation period, it was not watered for 8 consecutive days, and then two kinds of spots were collected. For the leaves of the wild plant of Mao Mao, the leaves of the wild plant of Mao Mao in th...

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Abstract

The invention discloses a gene SaWRKY expressed under drought stress in the wild species of P. chinensis, special primers for amplifying the gene and a method for detecting the differential expression of the gene under drought stress in the wild species of P. chinensis by relative quantitative RT-PCR technology. The invention provides technical support for drought-resistant breeding of sugarcane.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to the WRKY transcription factor gene SaWRKY under drought stress, and the special primers for amplifying the gene, and detecting the differential expression of the protein gene in the wild species of sarcophagus by relative quantitative RT-PCR technology method. Background technique [0002] Spotted grass (Erianthus arundinaceus (Retz.) Jeswiet) is one of the wild relatives of sugarcane, also known as Dami, Bamao, and Dabamao. It has excellent characteristics such as strong ability and is a material with great utilization value in sugarcane breeding. The success or failure of breeding depends to a large extent on the possession of raw materials for breeding and the breadth and depth of research and utilization. Therefore, the collection of wild germplasm resources of Banmao chinensis is highly valued by sugarcane breeding experts. In recent years, the researc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/11C12Q1/6895C12Q1/6851
CPCC07K14/415C12Q1/6851C12Q1/6895C12Q2600/13C12Q2600/158C12Q2531/113C12Q2545/101
Inventor 李富生肖芙荣何丽莲曹哲群陈疏影徐荣王先宏孟玉刘鲁峰狄义宁
Owner YUNNAN AGRICULTURAL UNIVERSITY