Cell protective agent as well as preparation method and application thereof
A protective agent and cell technology, applied in applications, preservation of human or animal bodies, animal husbandry, etc., can solve problems such as death, inability to experiment or detect, and achieve the effects of wide temperature range, long storage time, and convenient transportation
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Embodiment 1
[0024] 1. Preparation of Cell Protectant
[0025] Prepare 10mmol / L, 20mmol / L, 50mmol / L and 100mmol / L Tris buffers with ultrapure water respectively, add 9g / L sodium chloride and 20g / L respectively to the above four buffer solutions L of glucose, 10g / L of sucrose, 10g / L of alanyl glutamine. Adjust the pH to 7.1-7.5 with 6mol / L HCl solution. Obtain cytoprotectant No. 1-4.
[0026] 2. Experimental design
[0027] 2.1 Collect 5 cases of healthy human blood with heparin lithium anticoagulant tubes, each case of blood 10mL, divide each case of blood into 5 parts, and mix the above 4 kinds of cytoprotective agents with each part of blood at a volume ratio of 1:50, set aside One part of blood without protective agent was used as control group.
[0028] 2.2 After the 5 parts of blood were placed at room temperature or transported for 48 hours, 1 mL was added to the positive stimulator stimulation tube of the Mycobacterium tuberculosis-specific cellular immune response detection kit...
Embodiment 2
[0051] 1. Preparation of Cell Protectant
[0052]Prepare 20mmol / L tris buffer solution with ultrapure water, add 9g / L sodium chloride, 10g / L sucrose, 10g / L alanyl glutamine to the above buffer solution, and then Add 10g / L, 20g / L, 30g / L, 40g / L glucose respectively, and adjust the pH to 7.1-7.5 with 6mol / L HCl solution. Obtain protective agent No. 5-8.
[0053] 2. Experimental design
[0054] 2.1 Collect 5 cases of healthy human blood with heparin lithium anticoagulant tubes, each case of blood 10mL, divide each case of blood into 5 parts, and mix the above 4 kinds of cytoprotective agents with each part of blood at a volume ratio of 1:50, set aside One part of blood without protective agent was used as control group.
[0055] 2.2 After the 5 parts of blood were placed at room temperature or transported for 48 hours, 1 mL was added to the positive stimulator stimulation tube of the Mycobacterium tuberculosis-specific cellular immune response detection kit (enzyme-linked immun...
Embodiment 3
[0065] 1. Preparation of Cell Protectant
[0066] Prepare 20mmol / L tris buffer solution with ultrapure water, add 9g / L sodium chloride, 20g / L glucose, 10g / L sucrose to the above buffer solution, and then add 1g / L , 5g / L, 10g / L alanyl glutamine, adjust the pH to 7.1-7.5 with 6mol / L HCl solution. Obtain protective agent No. 9-11.
[0067] 2. Experimental design
[0068] 2.1 Collect 5 cases of healthy human blood with lithium heparin anticoagulant tubes, 10 mL of blood per case, divide each case of blood into 4 parts, mix the above three protective agents with each part of blood at a volume ratio of 1:50, and reserve a A portion of blood without protective agent was used as a control group.
[0069] 2.2 After 5 parts of blood were placed at room temperature or transported for 48 hours, 1 mL was added to the positive stimulator stimulation tube of the Mycobacterium tuberculosis-specific cellular immune response detection kit (enzyme-linked immunoassay), and the IGRA in the kit ...
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