Microsatellite marking method for parentage identification of coreius guichenoti
A technology of microsatellite marking and copperfish, which is applied to biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of no application of microsatellite marking, optimize the setting of annealing temperature, reduce time cost, The effect of improving the amplification effect
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[0023] In the following, the present invention will be further explained in conjunction with examples.
[0024] A microsatellite labeling method for paternity identification of copperfish, its steps are:
[0025] (1) Extract the DNA of the parental and offspring individuals of copperfish: Collect 8 family samples (including 22 true parents and 297 offspring), and select 20 non-parental samples as candidate parents, of which 8 family samples Including 5 full-sib families, 1 half-sibling family and 2 mixed families. Use absolute ethanol to preserve the fin ray tissue of the copperfish parent and the individual samples of the whole fish from which the offspring have just hatched from the membrane. Use Omega Bio-Tek's Tissue DNA Kit (D3396) to extract the genomic DNA of the sample. The specific steps are as follows :Choose about 30 mg of round-mouth copper fish fin strips or whole fish samples, wash them with double distilled water, put them in a 1.5mL sterile centr...
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