Vegetable pesticide residue detection method

A technique for detecting pesticide residues and vegetables, which is applied in the direction of analyzing materials through chemical reactions and analyzing materials through observation of the influence of chemical indicators. Improved readiness rate and easy operation

Inactive Publication Date: 2018-12-14
常州常检一诺食品检测中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the determination structure accuracy rate of the existing enzyme inhibition rate method is low, the purpose of the present invention is to provide a method for the detection of pesticide residues in vegetables, which has the advantages of fast determination speed and simple operation

Method used

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  • Vegetable pesticide residue detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0021] A method for detecting pesticide residues in vegetables, comprising the steps of:

[0022] S1. Reagent preparation and preparation: Dissolve 1 bag of buffer in 1000mL of pure water when configuring buffer, and store at room temperature; Dissolve and store at 0-4°C; dissolve each bottle of substrate powder with 10mL buffer solution when preparing the substrate, and store at 0-4°C; dissolve each bottle of chromogenic agent with 10mL buffer solution when preparing the chromogenic reagent, and store it in a dark place;

[0023] S2. Sample preparation: take vegetables with a clean surface, take the leaves of leafy vegetables, accurately weigh 1g of leaves with a balance, cut the leaves into small pieces of 1cm square size, put them into an extraction bottle, add 20mL of buffer solution, shake for 1min, and get The extract was allowed to stand still for 4 minutes, and the supernatant was obtained for subsequent use;

[0024] S3. Detection of control solution: Take 3ml of buf...

Embodiment 2

[0031] A method for detecting pesticide residues in vegetables, comprising the steps of:

[0032] S1. Reagent preparation and preparation: Dissolve 1 bag of buffer in 1000mL of pure water when configuring buffer, and store at room temperature; Dissolve and store at 0-4°C; dissolve each bottle of substrate powder with 10mL buffer solution when preparing the substrate, and store at 0-4°C; dissolve each bottle of chromogenic agent with 10mL buffer solution when preparing the chromogenic reagent, and store it in a dark place;

[0033] S2. Sample preparation: Take melons and fruits with a clean surface, take the fruit part of the melons and fruits, accurately weigh 2g of melons and fruits with a balance, cut the melons and fruits into small pieces of 1cm square size, put them into the extraction bottle, add 20mL of buffer solution, and shake 2min, to obtain the extract, stand still for 5min, to obtain the supernatant for later use;

[0034] S3. Detection of control solution: Take ...

Embodiment 3

[0041] A method for detecting pesticide residues in vegetables, comprising the steps of:

[0042] S1. Reagent preparation and preparation: Dissolve 1 bag of buffer in 1000mL of pure water when configuring buffer, and store at room temperature; Dissolve and store at 0-4°C; dissolve each bottle of substrate powder with 10mL buffer solution when preparing the substrate, and store at 0-4°C; dissolve each bottle of chromogenic agent with 10mL buffer solution when preparing the chromogenic reagent, and store it in a dark place;

[0043] S2. Sample preparation: take vegetables with a clean surface, take leaf parts from leafy vegetables, accurately weigh 1g of leaves with a balance, cut the leaves into small pieces of 1cm square size, put them into an extraction bottle, add 20mL of buffer solution, and shake for 3min to obtain The extract was allowed to stand still for 6 minutes, and the supernatant was obtained for subsequent use;

[0044] S3. Detection of control solution: Take 3ml...

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Abstract

The invention provides a vegetable pesticide residue detection method, and particularly relates to the technical field of pesticide residue detection. The method comprises the following steps of S1, performing reagent preparation: preparing a buffer solution, a butyrylcholinesterase solution, a substrate and a color developing agent; S2, performing sample preparation: cutting leaves, melon pulp orthe like into small blocks, putting the small blocks into an extraction bottle, adding the buffer solution, shaking to obtain an extracting solution, and standing to obtain a supernate for later use;S3, performing control solution detection: adding the buffer solution and the butyrylcholinesterase solution into a reaction flask, adding the color developing agent, performing placement for a certain time in a constant-temperature incubator, adding the substrate, pouring a mixture into a cuvette, and carrying out detection by using a pesticide residue fast detection instrument; S4, performing to-be-detected sample solution detection: taking the supernate, the butyrylcholinesterase solution and the color developing agent, performing placement in the constant-temperature incubator for a period of time, pouring a mixture into the cuvette, shaking, and carrying out detection by using the pesticide residue fast detection instrument; and S5, calculating a detection result. The method has theadvantages that the preparation rate is increased, the detection speed is high, and the operation is simple.

Description

technical field [0001] The invention belongs to the technical field of pesticide residue detection, and in particular relates to a method for detecting pesticide residues in vegetables. Background technique [0002] In our country, pesticide residues in vegetables and fruits are extremely serious. The main reason for the excessive pesticide residues in fruits and vegetables is that some farmers use highly toxic pesticides that are strictly prohibited by the state in the cultivation of fruits and vegetables, resulting in excessive pesticide residues. Eating fruits and vegetables with excessive toxic pesticide residues will cause human and animal poisoning incidents and cause great harm to human bodies. At this stage, there are many rapid detection methods for pesticide residues in my country, which are mainly divided into two categories: biochemical detection methods and chromatographic detection methods. The chromatographic detection method is accurate in determining the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 胡月珍耿云娣曹鑫宗宁燕
Owner 常州常检一诺食品检测中心有限公司
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