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Indoor identification method for resistance of peanut pods to Aspergillus flavus infection and application of indoor identification method

An indoor identification and Aspergillus flavus technology, applied in the direction of material inspection, food testing, etc., can solve the problems of microbial pollution, a large amount of labor, difficult peanut resistance level, etc., and achieve the effect of easy implementation and simple operation

Pending Publication Date: 2018-12-14
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

1) Identify the resistance of peanuts to Aspergillus flavus infection by inoculating peanut plants in the field. This method requires a long period of identification and requires a lot of labor, and is susceptible to contamination by microorganisms in the environment; The heavy method is used to detect the degree of Aspergillus flavus infection. Although this method is simple to operate, the protein, oil content, oleic acid, and linoleic acid content of different peanut varieties will change to varying degrees after being infected by Aspergillus flavus. As a result, the error of the Aspergillus flavus content results is relatively large, and it is difficult to distinguish the resistance level of peanuts after the Aspergillus flavus infection; 3) By manually observing the number and degree of Aspergillus flavus infection in peanut kernels, it can be more accurate Identification of Peanut Kernel Resistance to Aspergillus flavus

Method used

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  • Indoor identification method for resistance of peanut pods to Aspergillus flavus infection and application of indoor identification method
  • Indoor identification method for resistance of peanut pods to Aspergillus flavus infection and application of indoor identification method
  • Indoor identification method for resistance of peanut pods to Aspergillus flavus infection and application of indoor identification method

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Effect test

Embodiment 1

[0058] (1) Select the Aspergillus flavus strain: use the Aspergillus flavus strain AF2202 (Institute of Oil Crops, Chinese Academy of Agricultural Sciences) as the inoculation strain with high toxin production and strong infectivity; inoculate the Aspergillus flavus strain AF2202 in PDA medium, 30 ℃ dark Cultivate for 7 days, collect conidia with sterile mass concentration of 0.1% Tween 80 solution when inoculating, and resuspend conidia, adjust the concentration of spores to 2×10 6 spore / mL to obtain the Aspergillus flavus spore suspension (as the Aspergillus flavus spore inoculation suspension for subsequent use);

[0059] (2) Selection, sterilization and rehydration of peanut pods: Peanut germplasm resources HP17, 16-61731, 16-61722, 16-61534 (Institute of Oil Crops, Chinese Academy of Agricultural Sciences) were selected as test materials (ie peanut varieties) , high-sensitivity peanut germplasm resource 16-61594 (Institute of Oil Crops, Chinese Academy of Agricultural Sci...

Embodiment 2

[0071](1) Select the Aspergillus flavus strain: use the Aspergillus flavus strain AF2202 (Institute of Oil Crops, Chinese Academy of Agricultural Sciences) as the inoculation strain with high toxin production and strong infectivity; inoculate the Aspergillus flavus strain AF2202 in PDA medium, 30 ℃ dark Cultivate for 7 days, collect conidia with sterile mass concentration of 0.1% Tween 80 solution when inoculating, and resuspend conidia, adjust the concentration of spores to 2×10 6 spore / mL to obtain the Aspergillus flavus spore suspension (as the Aspergillus flavus spore inoculation suspension for subsequent use);

[0072] (2) Selection, sterilization and rehydration of peanut pods: Peanut germplasm resources 16-61730, 16-61714, 16-61639, 16-61689 (Institute of Oil Crops, Chinese Academy of Agricultural Sciences) were selected as test materials. Peanut germplasm resource 16-61594 (Institute of Oil Crops, Chinese Academy of Agricultural Sciences) was used as a control. 60 hea...

Embodiment 3

[0082] (1) Select the Aspergillus flavus strain: use the Aspergillus flavus strain AF2202 (Institute of Oil Crops, Chinese Academy of Agricultural Sciences) as the inoculation strain with high toxin production and strong infectivity; inoculate the Aspergillus flavus strain AF2202 in PDA medium, 30 ℃ dark Cultivate for 7 days, collect conidia with sterile mass concentration of 0.1% Tween 80 solution when inoculating, and resuspend conidia, adjust the concentration of spores to 2×10 6 spore / mL to obtain the Aspergillus flavus spore suspension (as the Aspergillus flavus spore inoculation suspension for subsequent use);

[0083] (2) Selection, sterilization and rehydration of peanut pods: Peanut germplasm resources 16-61602, 16-61600, 16-61601, 16-61598 (Institute of Oil Crops, Chinese Academy of Agricultural Sciences) were selected as test materials. Peanut germplasm resource 16-61594 (Institute of Oil Crops, Chinese Academy of Agricultural Sciences) was used as a control. 60 he...

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Abstract

The invention relates to an indoor identification method for resistance of peanut pods to Aspergillus flavus infection and an application of the indoor identification method to screening of peanut germplasm resources with resistance to Aspergillus flavus infection. The indoor identification method for resistance of the peanut pods to Aspergillus flavus infection is characterized by comprising thefollowing steps: (1) selecting an Aspergillus flavus strain; (2) selecting, sterilizing and rehydrating the peanut pods; (3) inoculating the peanut pods with Aspergillus flavus: healthy peanut pods are inoculated with an Aspergillus flavus spore suspension; (4) performing culture: dark culture is performed in an incubator for 7 days after inoculation; (5) classifying the cultured peanut pods according to infection degree; (6) calculating the Aspergillus flavus MOI (multiplicity of infection) of the peanut pods; (7) evaluating resistance levels. The method is simple to operate, easy to implement, can identify resistance of the peanut pods to Aspergillus flavus infection efficiently and accurately in a batch manner and is suitable for batch screening of the peanut germplasm resources with resistance to Aspergillus flavus infection. The foundation is laid for researching the resistance mechanism of the peanut pods to Aspergillus flavus infection and breeding peanut varieties with resistance to Aspergillus flavus infection.

Description

technical field [0001] The invention relates to an indoor identification method for resistance of peanut pods to Aspergillus flavus infection and its application in screening peanut germplasm resources resistant to Aspergillus flavus infection. Background technique [0002] Aflatoxin, a secondary metabolite produced by Aspergillus flavus infecting peanuts, has always been an important factor restricting peanut production worldwide. After eating food contaminated by aflatoxin, it can cause human and animal poisoning and induce liver cancer, and our country is One of the countries with serious aflatoxin pollution. Breeding varieties resistant to Aspergillus flavus is the most effective, economical and safest method to solve the pollution of peanuts caused by Aspergillus flavus and aflatoxin, and establishing a rapid and accurate identification method for peanuts resistant to Aspergillus flavus infection will help to speed up the development of peanut varieties resistant to Asp...

Claims

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Application Information

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IPC IPC(8): G01N33/02
CPCG01N33/02
Inventor 康彦平廖伯寿姜慧芳雷永晏立英万丽云淮东欣邱西克
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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