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ZmCLA2-1 gene for regulating included angle between corn leaves, and application thereof

A technology of corn leaf angle and genetics, applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of compact plant type and small leaf angle

Active Publication Date: 2018-12-18
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a method for regulating the included angle of corn leaves. ZmCLA2-1 The gene and its application solve the problems encountered in the cultivation of excellent high-yielding maize varieties with dense plant type and dense tolerance characteristics in the prior art

Method used

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  • ZmCLA2-1 gene for regulating included angle between corn leaves, and application thereof
  • ZmCLA2-1 gene for regulating included angle between corn leaves, and application thereof
  • ZmCLA2-1 gene for regulating included angle between corn leaves, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Construction of near-isogenic lines

[0030] The maize inbred line Yu 82 with small leaf angle and compact plant type was used as the donor parent, and the maize inbred line Yu 87-1 with large leaf angle and loose plant type was used as the recipient parent through backcross transfection combined with molecular markers Assisted selection technique to construct near-isogenic lines ( figure 1 ). In the spring of 2006, the combination of two parents in Zhengzhou won F 1 ; F planted in Hainan in the winter of 2007 1 Progeny and recipient parent Yu 87-1, backcross F with Yu 87-1 1 get BC 1 f 1 ;according to BC 1 f 1 The phenotype selection of 21 plants with small leaf angles was planted in Zhengzhou in the spring of 2008 to form ear rows, and continued to be backcrossed with Yu 87-1 to obtain BC 2 f 1 ; Selected 18 individual plants with small leaf angles and planted them in Sanya, Hainan in the winter of 2008, and then continued to backcross with Yu 87-...

Embodiment 2

[0031] Embodiment 2: Adopt map bit cloning technology to separate ZmCLA2-1 Gene

[0032] Use BC 3 f 2 The population of 6529 strains was used as a fine-positioning population, and the single strain 720 with a leaf angle less than or equal to 15° was selected from it. QLA1 Two-sided markers umc1165 and bnlg1297 were used to analyze their genotypes, and 18 individual plants with recombination and exchange at umc1165 or bnlg1297 were selected; at the same time, 608 individual plants with leaf angles greater than 20° were selected, and 5 were screened out by combining molecular marker selection. Exchange individual plants with large leaf angles. Using newly developed 17 pairs of primers SSR2-1, SSR2-2, SSR2-3, SSR2-4, SSR2-5, SSR2-6, SSR2-24, SSR2-25, SSR2-26, SSR2 with strong polymorphism -27, SSR2-28, SSR2-36, SSR2-37, SSR2-38, SSR2-39, SSR2-40, SSR2-54 (marker development methods refer to Zhang Jun, ZmCLA4 gene map-site cloning and Functional Analysis, Ph.D. Dissertation ...

Embodiment 3

[0035] Example 3: ZmCLA2-1 Negative regulation of maize leaf angle

[0036] ZmCLA2-1 The leaf angle was increased in Yu87-1, and the leaf angle was decreased in Yu87-1-NIL58, so we detected the expression of ZmCLA2-1 gene. The results of qRT-PCR detection showed that the leaves from the 10 to 12 leaf stages of maize growth and development ( image 3 ) efficient expression. For different materials, ZmCLA2-1 The expression level of Yu87-1 in different periods was significantly lower than that of Yu87-1-NIL58 ( image 3 ),show ZmCLA2-1 The leaf angle of maize with high expression level is relatively reduced, so it is proposed that ZmCLA2-1 Regulates the size of the maize leaf angle as a negative regulator.

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Abstract

The invention relates to the field of crop genetic breeding, and especially relates to a ZmCLA2-1 gene for regulating an included angle between corn leaves, and an application thereof. An overexpression technique is used to promote the expression of endogenous ZmCLA2-1 gene in corn, so pulvinus position apo-axle terminalsustentacular tissues are developed, the lignification degree is high, thick-walled cells have many layers, are small and have a large quantity, vascular bundles are increased, and the included angle between the leaves is reduced. The ZmCLA2-1 gene is fused with a constitutivepromoter to construct a vector for promoting gene expression, and a corn inbred line with a small leaf angle and a compact plant type is created by a transgenic technology and is used for cultivatinga new density-resistant corn variety. A gene containing a sequence represented by SEQ ID NO:1 is reconstructed, and then is introduced into the corn plant to create a corn breeding material with a compact plant type and a small leaf angle, so the ZmCLA2-1 gene has great practical application significance to corn breeding.

Description

technical field [0001] The invention relates to the field of crop genetics and breeding, in particular to a method for regulating the included angle of corn leaves. ZmCLA2-1 Genes and their applications. Background technique [0002] In recent years, the area of ​​arable land has gradually decreased, and factors such as the balance between the planting area of ​​various crops and actual demand have also led to the reduction of the planting area of ​​corn. However, the corn required for national development continues to increase. On the limited arable area, only by increasing the yield per unit area and then increasing the total yield can meet social needs and ensure national food security. Increasing the planting density by cultivating good density-tolerant maize varieties is proposed as a method to increase the yield per unit area. The new high-yield maize varieties with tolerance to density must have the plant type and characteristics of tolerance to density. A compact ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8205C12N15/8261
Inventor 陈彦惠库丽霞曹莹莹任真真
Owner HENAN AGRICULTURAL UNIVERSITY
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