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Plasminogen immunoturbidimetry detection kit

A technology of plasmin and detection kits, which is applied in the field of plasminogen immunoturbidimetric detection kits, can solve the problems of poor stability, low accuracy and sensitivity of detection reagents, and achieve anti-interference ability Strong, optimized reaction system, and enhanced stability

Inactive Publication Date: 2018-12-18
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The detection of plasminogen is mainly immunoturbidimetric method, but this method uses antibodies, so the ordinary fibrin immunoturbidimetric assay reagents are not stable, and the accuracy and sensitivity are not high, thus limiting its use. Clinical application

Method used

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  • Plasminogen immunoturbidimetry detection kit

Examples

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Embodiment 1

[0020] A conventional detection kit for plasminogen immunoturbidimetric method, comprising reagent R1, reagent R2, and calibrator.

[0021] Wherein the reagent R1 consists of:

[0022] pH 7.6Mes buffer 100mmol / L

[0023] Triton X-100 25ml / L

[0024] Reagent R2 consists of:

[0025] pH 7.6Mes buffer 100mmol / L

[0026] Goat anti-human Pg antibody 30ml / L

[0027] Sodium azide 7.7mmol / L

[0028] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, and the operation is as follows:

[0029] Add 3 μl of normal saline, sample or calibrator, then add 225 μl of R1 reagent for pre-incubation for 5 minutes and read the absorbance A1, then add 75 μl of reagent R2 and react for 5 minutes, read the absorbance A2, and calculate ΔA.

[0030] The calibrator used in this example is the Pg calibrator produced by Jiufeng Runda Biotechnology Co., Ltd.

Embodiment 2

[0032] A detection kit for plasminogen immunoturbidimetric method, comprising reagent R1, reagent R2 and calibrator.

[0033] Wherein the reagent R1 consists of:

[0034] pH 7.6Mes buffer 100mmol / L

[0035] Triton X-100 25ml / L

[0036] Trehalose 10g / L

[0037] Reagent R2 consists of:

[0038] pH 7.6Mes buffer 100mmol / L

[0039] Goat anti-human Pg antibody coated latex particles 30ml / L

[0040] Sodium azide 7.7mmol / L

[0041] Concrete determination method is with embodiment 1.

Embodiment 3

[0043] A detection kit for plasminogen immunoturbidimetric method, comprising reagent R1, reagent R2 and calibrator.

[0044] Wherein the reagent R1 consists of:

[0045] pH 7.6Mes buffer 100mmol / L

[0046] Triton X-100 25ml / L

[0047] Trehalose 20g / L

[0048] Reagent R2 consists of:

[0049] pH 7.6Mes buffer 100mmol / L

[0050] Goat anti-human Pg antibody coated latex particles 30ml / L

[0051] Sodium azide 7.7mmol / L

[0052] Concrete determination method is with embodiment 1.

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Abstract

The invention provides a plasminogen immunoturbidimetry detection kit. Trehalose is added into a reagent R1, so that the reaction system is more viscous, and relatively neutral, and the problem that the antibody is not stable in a dilute solution is solved to a certain extent, thereby playing a certain role in protecting the antibody; meanwhile, goat anti-human Pg antibody coated latex particles,instead of a goat anti-human Pg antibody, are added into a reagent R2, so that the antibody is protected fundamentally. Therefore, by virtue of the joint effect of the trehalose and the latex particles, the stability of the kit is effectively enhanced, influence to the accuracy of the reagent is avoided, and further popularization of the reagent in the market is promoted.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a plasminogen immunoturbidimetric detection kit. Background technique [0002] Human plasminogen is the precursor substance of human plasmin and has no activity. In normal human body, human plasminogen is converted into active human plasmin by activation of plasminogen activator. Human plasminogen (Plasminogen, Pg) is a kind of plasma protein, mainly produced by liver cells, and the content in plasma is 0.06-0.25mg / mL. This protein can be activated by plasminogen activator, transform into active plasmin and play various physiological roles. [0003] Plasminogen is the precursor form of plasmin, which is activated by activators, and then hydrolyzes fibrin (ogen) and other coagulation factors to play an anticoagulant role. The role of plasmin is to maintain the balance of blood coagulation and fibrinolysis in blood vessels, so as to make the blood fl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 甘宜梧谢清华段洪东胡晓飞李志明赵民
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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