Dendritic poly amidoamine and graphene oxide combined novel composite material, and preparation method and application thereof
A polyamidoamine and composite material technology, applied in the fields of nano-biomaterials and nano-medicine, can solve the problems of easy generation of immune response, reduced safety, and high cytotoxicity, and achieves high transfection effect, accelerated endosome escape, and cytotoxicity. low effect
Active Publication Date: 2018-12-21
ZHUHAI INST OF ADVANCED TECH
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Problems solved by technology
Viral vectors have high transfection efficiency, but due to strong immunogenicity, they are prone to immune reactions and reduce safety
Traditional non-viral vectors include transfection reagents such as liposomes, inorganic nanoparticles, and cationic polymers, but such vectors also have the disadvantages of low efficiency and high cytotoxicity
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Embodiment 1
[0038] Embodiment 1 of the present invention is: a polyamidoamine combined with graphene oxide novel composite material (GO-PAMAM-PEG), the preparation raw material of described composite material comprises the nanometer graphene oxide of 1 weight part, the branch of 20 weight parts Polyamidoamine (the polymer part is -CH 2 -CH 2 -O-), polyethylene glycol and EDC of 10 parts by weight, dendritic polyamidoamine and polyethylene glycol are combined on the surface of nano-graphene oxide by cross-linking agent to make the composite material, wherein, the polyethylene glycol The diol is an aminated methoxy polyethylene glycol derivative (MPEG-NH) with a molecular weight of 2000 2 ), the dendritic polyamidoamine molecular weight is 517, and the end contains 4 amino groups.
[0039] The preparation method of above-mentioned composite material specifically comprises the following steps:
[0040] (1) Ultrasonically dissolve nano-scale graphene oxide in water, add EDC and dendritic p...
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Abstract
The invention relates to a dendritic poly amidoamine and graphene oxide combined novel composite material, and a preparation method and application thereof. The composite material comprises nano graphene oxide, dendritic poly amidoamine, polyethylene glycol and a crosslinking agent, wherein dendritic poly amidoamine is modified on the surface of nano graphene oxide by the crosslinking agent, and polyethylene glycol is bonded to the surface of nano graphene oxide by the crosslinking agent; polyethylene glycol has molecular weight of 1,000-5,000, and in the structure of polyethylene glycol, oneend is an amino group, and the other end is a methoxy group; dendritic poly amidoamine has molecular weight of 2,000 or less, and has less than 10 terminal amino groups. Compared with the prior art, the composite material provided by the invention has the advantages of high specific surface area, good biocompatibility and good solubility.
Description
technical field [0001] The invention relates to the technical fields of nanobiological materials and nanomedicine, in particular to a novel composite material of dendritic polyamidoamine combined with graphene oxide and its preparation method and application. Background technique [0002] In the field of biomedicine, using gene therapy as a means of treatment, the introduction of functional foreign genes into cells containing gene deletions / mutations and effective expression to change genetic characteristics can achieve better therapeutic effects, and this technology has become an important means of treatment. Efficient gene therapy is inseparable from efficient gene transfer carriers. Current gene delivery methods can generally be divided into two categories: viral vectors and non-viral vectors. Viral vectors have high transfection efficiency, but due to strong immunogenicity, they are prone to immune reactions and reduce safety. Traditional non-viral vectors include tra...
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IPC IPC(8): C08L79/02C08L71/00C08K3/04C08K5/29C08K5/3415C08J3/24C12N15/87
CPCC08J3/24C08J2379/02C08J2471/00C08K2201/011C08L79/02C12N15/87C08L71/00C08K3/042C08K5/29C08K5/3415
Inventor 张溪之谢苍桑李军华张利鹏徐畅姜长安
Owner ZHUHAI INST OF ADVANCED TECH