Breeding method and device for improving survival rate of juvenile zebra fish

A breeding method and zebrafish technology, applied in fish farming, climate change adaptation, animal feed, etc., can solve problems such as different survival rate and quality of juvenile fish, different breeding and breeding methods, and different quality of embryos, so as to ensure survival and growth, easy to use and maintain, and low cost

Inactive Publication Date: 2018-12-28
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the breeding and breeding methods of zebrafish adopted by various research institutions are different, and the quality of each batch of embryos is different, and the survival rate and quality of juvenile fish are also different, which ultimately leads to poor repeatability of experimental results.

Method used

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  • Breeding method and device for improving survival rate of juvenile zebra fish
  • Breeding method and device for improving survival rate of juvenile zebra fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Breed 50 zebrafish embryos in the middle of the box of the above-mentioned device for improving the survival rate of juveniles, blow the embryos away with a disposable dropper, so that the embryos do not adhere to each other, and remove dead embryos in the incubator in time ;

[0036] (2) hatch the zebrafish embryos, and the zebrafish larvae will all hatch after about 72 hours;

[0037] (3) After hatching, the temperature of the water body in the control device is 25°C, and the pH value of the water quality is controlled at 7; the juveniles are fed with egg yolk water from the first day, and the juveniles are fed with freshly hatched Artemia after 7 days;

[0038] (4) The experimental culture period is one month, and the survival rate of juvenile fish is counted.

Embodiment 2

[0040](1) Breed 70 zebrafish embryos in the middle of the box of the above-mentioned device for improving the survival rate of juveniles, blow the embryos away with a disposable dropper, so that the embryos do not adhere to each other, and remove dead embryos in the incubator in time ;

[0041] (2) hatch the zebrafish embryos, and the zebrafish larvae will all hatch after about 72 hours;

[0042] (3) After hatching, the temperature of the water body in the control device was 26°C, and the pH value of the water quality was controlled at 7.5; the juveniles were fed egg yolk water from the second day, and the juveniles were fed with freshly hatched Artemia after 8 days;

[0043] (4) The experimental culture period is one month, and the survival rate of juvenile fish is counted.

Embodiment 3

[0045] (1) Breed 80 zebrafish embryos in the middle of the box of the above-mentioned device for improving the survival rate of juveniles, blow the embryos away with a disposable dropper, so that the embryos do not adhere to each other, and remove dead embryos in the incubator in time ;

[0046] (2) hatch the zebrafish embryos, and the zebrafish larvae will all hatch after about 72 hours;

[0047] (3) After hatching, the temperature of the water body in the control device was 27°C, and the pH value of the water quality was controlled at 7.5; the juvenile fish were fed egg yolk water from the third day, and the juvenile fish were fed with freshly hatched Artemia after 10 days;

[0048] (4) The experimental culture period is one month, and the survival rate of juvenile fish is counted.

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PUM

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Abstract

The invention discloses a breeding method and device for improving the survival rate of juvenile zebra fish. The breeding method comprises the steps that healthy adult zebra fish is selected as parents; male zebra fish and female zebra fish are taken and put in a breeding tank for breeding; the embryos in a lower layer of the breeding tank are collected and put in a breeding box and are blown awayby using a disposable dropper, and dead embryos in the breeding box are removed in time; the embryos are hatched, and all juvenile fish is fed with yolk water after being hatched; the yolk water is fed (for 7-10 days), and then the juvenile fish is fed with fresh hatched fairy shrimps. The breeding device is a breeding box and comprises a box body, an upper partition plate, a lower partition plate and a horn-shaped feeding port. The upper partition plate and the lower partition plate are parallelly placed on protrusions arranged on the inner wall of the breeding box body, and the box body isdivided into an upper portion, a middle portion and a lower portion. The feeding port is fixed to the upper partition plate. The upper partition plate and the lower partition plate are both provided with screen holes. By adopting the breeding method and the breeding device for breeding zebra fish, the survival rate of juvenile zebra fish can be remarkably improved, the cost is low, and the operation is simple and convenient.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a breeding method for improving the survival rate of juvenile zebrafish. Background technique [0002] Zebrafish is a typical freshwater fish. Due to the characteristics of small embryo size, transparency, easy feeding, high yield, short growth cycle, continuous production capacity, low breeding cost, and less experimental consumables, zebrafish has become a fish. A model for species toxicology studies. The body of the zebrafish has uniform blue stripes extending from the mouth to the caudal fin, which is fusiform in shape and compressed laterally. Zebrafish can grow up to 6.4 cm in length, but rarely grow larger than 4 cm. Zebrafish generally live within two to three years, extending up to five years under ideal conditions. The structure and function of fish and vertebrate organs are similar, therefore, the toxic effect of toxic substances on fish can be used to predict t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K63/00A23K50/80A23K10/20A01K61/59
CPCA01K61/10A01K61/59A01K63/003A23K10/20A23K50/80Y02A40/81
Inventor 杜佳
Owner HANGZHOU DIANZI UNIV
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