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Aminofullerene derivatives, preparation method and application thereof

An aminofullerene and fullerene technology, which is applied in the field of aminofullerene derivatives, can solve the problems of low water solubility and easy agglomeration of fullerene derivatives, and achieves good purification effect and good dispersibility. , the effect of easy operation

Active Publication Date: 2021-07-30
BEIJING FUNAKANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In addition, how to improve the preparation method of water-soluble fullerene derivatives, so that it can be conveniently controlled by adjusting the reaction temperature or reaction time, to control the number of modified groups bonded to the obtained water-soluble fullerene derivatives, and , can improve the water solubility, particle size and dispersibility of the prepared water-soluble fullerene derivatives, so as to solve the problems that the water-soluble fullerene derivatives currently prepared are easy to agglomerate and have low water solubility

Method used

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  • Aminofullerene derivatives, preparation method and application thereof
  • Aminofullerene derivatives, preparation method and application thereof
  • Aminofullerene derivatives, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1: Preparation C 60 (EDA) 6

[0074] (a) Weigh 50 mg of solid fullerene C with an analytical balance 60 (Purity: 99%, Xiamen Funa New Material Technology Co., Ltd.) was dissolved in 25mL of o-xylene solution, ultrasonically dispersed for 30min, and 10mL of ethylenediamine (analytical grade, national medicine reagent) was measured with a measuring cylinder, and the o-xylene The solution and ethylenediamine were added to a 250mL Erlenmeyer flask with a stopper, then a magnet was added, and stirred for 1h using a magnetic stirrer (temperature: room temperature, speed: 1000r / min, N 2 as a protective gas), let it stand, and collect the precipitate.

[0075] (b) Add 50mL of ethylenediamine (analytical grade, Sinopharm Reagent) to the collected precipitate, and stir for 24 hours (temperature: room temperature, rotating speed: 1000r / min, N 2 as protective gas).

[0076] (c) Add the solution obtained in step (b) into a 250ml round bottom flask, and then use a rotary...

Embodiment 2

[0087] Example 2: Preparation C 70 (EDA) 8

[0088] Reaction conditions and steps are the same as in Example 1, except that 50mg of fullerene C is weighed 70 Solid (purity: 99%, Xiamen Funa New Material Technology Co., Ltd.) instead of C 60 . finally obtained with C 70 (EDA) 8 clear reddish-brown solution.

[0089] Using the same test method and conditions as in Example 1, the obtained product was tested for components.

[0090] Such as figure 1 As shown, the characteristic infrared absorption of the sample around 3300nm (-NH 2 The stretching vibration absorption peak of the carbon cage) proves that ethylenediamine is bonded to the carbon cage of fullerene, and at the same time, the change of the infrared characteristic absorption at 800-1500nm (the stretching vibration peak of C-C and C=C on the carbon cage) also supports the change of ethylenediamine Already bonded to the carbon cage.

[0091] The sample solution is directly used in the dynamic light scattering (DL...

Embodiment 3

[0097] Example 3: C 60 (EDA) 6 、C 70 (EDA) 8 Hydroxyl radical scavenging test

[0098] Induction of H by ultraviolet (UV) 2 o 2 Hydroxyl radicals are generated, and DMPO is used as a free radical scavenger. DMPO quickly combines with free hydroxyl radicals to generate DMPO-OH, which can be detected by EPR.

[0099] Solution preparation: 100 μL of DMPO (100 mM) and 50 μL of HO 2 o 2 (100mM) and 50 μL of ultrapure water were mixed to prepare a blank control group.

[0100] test C 60 (EDA) 6、 C 70 (EDA) 8 When scavenging free radicals, the concentrations of samples added to the test system are: 20, 50, 100, 200, 400 μM.

[0101] see test results image 3 . C 60 (EDA) 6 、C 70 (EDA) 8 When the concentration is 50μM, there is an obvious effect of scavenging free radicals, when C 60 (EDA) 6 、C 70 (EDA) 8 When the concentration is 400 μM, the effect of scavenging hydroxyl free can reach 90%, and there is an obvious dose-effect relationship. For the convenience o...

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Abstract

The invention discloses an amino fullerene derivative and a preparation method thereof. The derivative has good water solubility, positive charge on the surface, suitable for cell phagocytosis, high free radical capture efficiency, and good free radical scavenging effect; and in a certain concentration range, not only has no cytotoxicity, but has a good cell protection effect. The production rate of the preparation method is high, and the purification treatment method is simple and convenient.

Description

technical field [0001] The invention belongs to the field of biomedical materials. Specifically, the present invention relates to an aminofullerene derivative, its preparation method and its application in the field of biomedicine, more specifically, the application relates to the scavenging of free radicals and the protection of Application of cells against free radical damage. Background technique [0002] Free radicals refer to groups whose outer orbitals contain unpaired electrons and are highly chemically active. [0003] Free radicals are inevitably produced in organisms, and in normal life processes, low levels of free radicals are necessary to maintain life. Free radicals in the body are constantly produced and eliminated, and the two are in a dynamic balance to maintain them at a normal physiological level. Studies have shown that a certain concentration of free radicals is one of the necessary conditions for the body to carry out normal life activities, but too ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C209/00C07C211/10A61K31/132A61P39/06
CPCC07C209/00C07C211/10
Inventor 王春儒马海军李杰甄明明
Owner BEIJING FUNAKANG BIOTECH CO LTD
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