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Novel nano magnetic particle suspension system and preparation method thereof

A technology of nano-magnetic particles and a preparation method, which is applied in the biological field, can solve the problems of slow sedimentation rate and poor dispersion stability of nano-magnetic particles, and achieve the effect of good paramagnetism and good dispersion stability

Inactive Publication Date: 2019-01-04
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of poor dispersion stability of nano-magnetic particles and slow sedimentation rate after magnetization in the prior art, the present invention provides a novel nano-magnetic particle suspension system, which is used to prepare and preserve nano-magnetic particles, and can improve the The sedimentation rate during the application of the magnetic field can also improve its dispersion stability under static conditions, which can effectively avoid the influence of the product performance due to the slow sedimentation rate and poor dispersion stability during the application of nano-magnetic particles

Method used

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  • Novel nano magnetic particle suspension system and preparation method thereof
  • Novel nano magnetic particle suspension system and preparation method thereof
  • Novel nano magnetic particle suspension system and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 1. The amount of raw materials and preparation steps of the new nano-magnetic particle suspension system

[0023] (1) Add 800mL of purified water into the container, weigh 0.1g of disodium hydrogen phosphate dodecahydrate and 0.1g of sodium dihydrogen phosphate dihydrate in the above-mentioned purified water, stir it with a magnetic stirrer for at least 15 minutes to completely dissolve it, and obtain Phosphate buffer solution, the pH range is 7.00±0.01;

[0024] (2) Weigh 5.0g of sodium chloride, 0.01g of 4-aminoantipyridine, and 1.0g of bovine serum albumin into the above buffer, and stir with a magnetic stirrer for at least 30 minutes to mix the solution evenly;

[0025] (3) Measure 10mL isopropanol, 0.01mL Triton X-100, 0.1mL Proclin-300 into the above solution, stir with a magnetic stirrer for at least 15 minutes to mix the solution evenly;

[0026] (4) Dilute the solution to 1 L with purified water, and filter it with a 0.22 μm filter to sterilize;

[0027] (5) ...

Embodiment 2

[0033] 1. The amount of raw materials and preparation steps of the magnetic particle suspension system

[0034] (1) Add 800mL of purified water into the container, weigh 1.0g of disodium hydrogen phosphate dodecahydrate and 0.5g of sodium dihydrogen phosphate dihydrate in the above-mentioned purified water, stir it with a magnetic stirrer for at least 15 minutes to completely dissolve it, and obtain Phosphate buffer solution, the pH range is 7.00±0.01;

[0035] (2) Weigh 20g of sodium chloride, 0.1g of 4-aminoantipyridine, and 20g of bovine serum albumin into the above buffer, and stir with a magnetic stirrer for at least 30 minutes to mix the solution evenly;

[0036] (3) Measure 20mL isopropanol, 0.1mL Triton X-100, 1.0mL Proclin-300 in the above solution, stir with a magnetic stirrer for at least 15 minutes to mix the solution evenly;

[0037] (4) Dilute the solution to 1 L with purified water, and filter it with a 0.22 μm filter to sterilize;

[0038] (5) Measure an appr...

Embodiment 3

[0044] 1. The amount of raw materials and preparation steps of the magnetic particle suspension system

[0045] (1) Add 800mL of purified water into the container, weigh 2.0g of disodium hydrogen phosphate dodecahydrate and 0.75g of sodium dihydrogen phosphate dihydrate in the above purified water, stir it with a magnetic stirrer for at least 15 minutes to completely dissolve it, and obtain Phosphate buffer solution, the pH range is 7.00±0.01;

[0046] (2) Weigh 30g of sodium chloride, 0.5g of 4-aminoantipyridine, and 30g of bovine serum albumin into the above buffer, and stir with a magnetic stirrer for at least 30 minutes to mix the solution evenly;

[0047] (3) Measure 40mL of isopropanol, 0.25mL of Triton X-100, and 2.5mL of Proclin-300 into the above solution, and stir with a magnetic stirrer for at least 15 minutes to mix the solution evenly;

[0048] (4) Dilute the solution to 1 L with purified water, and filter it with a 0.22 μm filter to sterilize;

[0049] (5) Meas...

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Abstract

The invention provides a novel nano magnetic particle suspension system and a preparation method thereof. According to the suspension system, with purified water as a solvent, every 1L of the suspension system contains 0.1-5 g of sodium phosphate dibasic dodecahydrate, 0.1-1 g of sodium dihydrogen phosphate dihydrate, 5-50 g of sodium chloride, 0.01-1.0 g of 4-aminoantipyrine, 1-50 g of bovine serum albumin, 10-50 mL of isopropanol, 0.01-0.5 mL of Triton X-100 and 0.1-5 mL of Proclin-300. The mass concentration of magnetic particles in the suspension system is 5.0%. The novel nano magnetic particle suspension system has good dispersion performance, the magnetic particles are evenly distributed, and the good dispersion and stabilization performance can be guaranteed; under the effect of themagnetic field, the nano magnetic particles can quickly settle, solid and liquid separation is quickly achieved, and the system has good paramagnetism.

Description

technical field [0001] The invention belongs to the field of biotechnology, and mainly relates to a novel nano-magnetic particle suspension system and a preparation method thereof. Background technique [0002] Magnetic separation technology is based on nano- or particle-level magnetic particles as a carrier, using the specific affinity characteristics provided by proteins bound to the surface of magnetic particles, under the directional control of a magnetic field, through affinity adsorption, cleaning, desorption and other operations , a technology to further separate and obtain target molecules from complex biological systems, has many advantages such as simple and convenient magnetic separation, high specificity and high sensitivity of affinity adsorption. The magnetic carrier used in magnetic separation technology should have the following characteristics: ① relatively small particle size, large specific surface area, and large adsorption capacity; ② stable physical and...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/68G01N33/58
CPCG01N33/54326G01N33/58G01N33/6854
Inventor 韩霜白仲虎张俊兰
Owner JIANGNAN UNIV
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