Bio sample pre-treatment device
A biological sample and pretreatment technology, applied in the preparation of test samples, biological testing, sampling, etc., can solve the problems of difficult to manufacture automatic equipment, difficult to reduce equipment size, non-automation and other problems
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0098] Example - Pretreatment Using Electromagnets
[0099] 200 mg of a cast stool sample and 1 ml of a pretreatment solution were put into a container together with a magnet (diameter: 2 mm), and after installing the electromagnet, pretreatment was performed at a current of 3 A, an oscillation frequency of 25 Hz, and a temperature of 85° C. for five minutes, and 200 µl of the solution was obtained through a membrane filter (pore diameter: 2 µm).
[0100] Comparative Example - Pretreatment with Vortex
[0101] 200 mg of cast stool sample and 1 ml of pretreatment solution were put into the container and they were mixed by using a vortexer until the sample was completely homogenized. The homogenized sample was heated at a temperature of 95° C. for five minutes by using a heating block and centrifuged at 14,500 rpm for one minute by using a centrifuge to obtain 200 μl of a solution.
experiment example
[0102] Experimental Example - Nucleic Acid Extraction and Analysis
[0103] Each 200 μl of the solution pretreated according to the example and the comparative example and 10 μl of the magnetic bead solution were put into 500 μl of the lysis / binding buffer, and allowed to react for 5 minutes. Use a magnetic rod to move only the magnetic particles into 750 μl of the first wash buffer and mix for one minute. After mixing, only the magnetic particles were moved into 750 μl of the second wash buffer using a magnetic rod and mixed for one minute. After mixing, only the magnetic particles were separated by using a magnetic bar and air dried for one minute. Dried magnetic particles were dropped into 200 μl of elution buffer and they were mixed for one minute. After mixing, only the magnetic particles were removed by using a magnetic bar and the rest of the solution was used for nucleic acid analysis. In order to minimize the deviation between the comparison methods according to ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More