Lentinan extraction method

A technology of lentinan and an extraction method, applied in the field of polysaccharide extraction, can solve the problems of low extraction rate and the like, and achieve the effect of improving the extraction rate

Inactive Publication Date: 2019-01-08
绩溪县徽菜宝生物科技有限公司
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a low extraction rate defect in the extraction of lentinan at present, therefore, it is very important to provide a method for extracting lentinan with a high extraction rate.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting lentinan, comprising the following steps:

[0020] S1: After cleaning the fresh shiitake mushrooms, cut them into small pieces and put them in a vacuum drying oven to dry. After the shiitake mushrooms have a constant mass, put them into a pulverizer to crush them, and pass through a 80-120 mesh sieve to obtain shiitake mushroom powder for later use;

[0021] S2: After mixing the shiitake mushroom powder obtained in step S1 with distilled water 15 times the weight of the shiitake mushroom powder, treat it at a temperature of 48° C. and a pressure of 20 MPa for 2 minutes, and release the pressure to obtain a paste-like slurry;

[0022] S3: heat the paste slurry to 50°C, keep it warm for 20 minutes, adjust the pH value to 5.5-6.5, add papain and cellulase, and perform ultrasonic treatment to obtain an extract;

[0023] S4: Suction filter the extract, compress the filtrate to 1 / 4 of the volume of the original filtrate, add 72% ethanol solution 10 tim...

Embodiment 2

[0028] A method for extracting lentinan, comprising the following steps:

[0029] S1: After cleaning the fresh shiitake mushrooms, cut them into small pieces and put them in a vacuum drying oven to dry. After the shiitake mushrooms have a constant mass, put them into a pulverizer to crush them, and pass through a 80-120 mesh sieve to obtain shiitake mushroom powder for later use;

[0030] S2: After mixing the shiitake mushroom powder obtained in step S1 with distilled water 20 times the weight of the shiitake mushroom powder, treat it at a temperature of 50° C. and a pressure of 25 MPa for 4 minutes, and release the pressure to obtain a paste slurry;

[0031] S3: heat the paste slurry to 53°C, keep it warm for 25 minutes, adjust the pH value to 5.5-6.5, add papain and cellulase, and perform ultrasonic treatment to obtain an extract;

[0032] S4: Suction filter the extract, compress the filtrate to 1 / 3 of the volume of the original filtrate, then add 75% ethanol solution 15 tim...

Embodiment 3

[0037] A method for extracting lentinan, comprising the following steps:

[0038] S1: After cleaning the fresh shiitake mushrooms, cut them into small pieces and put them in a vacuum drying oven to dry. After the shiitake mushrooms have a constant mass, put them into a pulverizer to crush them, and pass through a 80-120 mesh sieve to obtain shiitake mushroom powder for later use;

[0039] S2: After mixing the shiitake mushroom powder obtained in step S1 with distilled water 25 times the weight of the shiitake mushroom powder, treat it at a temperature of 55° C. and a pressure of 30 MPa for 5 minutes, and release the pressure to obtain a paste-like slurry;

[0040] S3: heat the paste slurry to 58°C, keep it warm for 30 minutes, adjust the pH value to 5.5-6.5, add papain and cellulase, and perform ultrasonic treatment to obtain an extract;

[0041] S4: Suction filter the extract, compress the filtrate to 1 / 4 of the volume of the original filtrate, then add 76% ethanol solution 2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a lentinan extraction method which comprises the following steps: mixing shii-take powder with distilled water, treating for 2-5 minutes under conditions that the temperatureis 48-55 DEG C and the pressure is 20-30 MPa, and releasing the pressure so as to obtain paste slurry; heating the paste slurry to 50-58 DEG C, keeping the temperature for 20-30 minutes, adjusting thepH value to 5.5-6.5, adding papain and cellulase, and carrying out ultrasonic treatment so as to obtain an extract; and carrying out suction filtration on the extract, concentrating the filtrate till1 / 4-1 / 3 of the original volume of the filtrate, carrying out alcohol separation, centrifuging, collecting precipitate, and carrying out vacuum freeze drying, thereby obtaining lentinan. By adopting the extraction method provided by the invention, the papain and the cellulase are compounded, the extraction rate of the lentinan can be increased due to synergistic effects of multiple enzymes, ultrasonic treatment is carried out in enzymolysis, the extraction rate of the lentinan can be further improved, and the lentinan extraction rate is as high as 17.2%.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, in particular to a method for extracting lentinan. Background technique [0002] Shiitake mushrooms belong to the phylum Fungi, Agaricaceae, Pleurotaceae, and the genus Lentinus in classification. Shiitake mushrooms are not only nutritious and delicious, but also have high medicinal value. They are one of the edible mushrooms with both medicinal and edible uses. [0003] Modern research shows that the main active ingredient in shiitake mushrooms is lentinan. Lentinan has various pharmacological activities such as anti-tumor, anti-aging, lowering blood sugar, improving human immunity and anti-oxidation. In recent years, research on lentinan has been a hot issue, mainly focusing on its extraction and activity research. However, the current extraction of lentinan has the disadvantage of low extraction rate. Therefore, it is very important to provide a method for extracting lenti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 胡永陆邵秋云陈敏钱玉明张媛程叙海章彦宇余建芳叶家东邵秀芳张英姿胡虹朱雅红程扶旗朱景娟范风华汪健胡在进
Owner 绩溪县徽菜宝生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap