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Pleurotus eryngii spawn rejuvenation screening method

A screening method, the technology of Pleurotus eryngii, which is applied in the field of Pleurotus eryngii strain rejuvenation screening, can solve the problems of long growth of Pleurotus eryngii, decline of Pleurotus eryngii, and affecting the benefits of mushrooms, etc.

Inactive Publication Date: 2019-01-08
江苏品品鲜生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for screening the rejuvenation of Pleurotus eryngii strains, to solve the problem that the Pleurotus eryngii proposed in the above-mentioned background technology is due to the long-term artificial culture. The push and slide of seed reproduction makes the growth of Pleurotus eryngii grow longer and the quality of formation becomes lower, which greatly affects the benefits of mushroom cultivation.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0018] A method for screening the rejuvenation of Pleurotus eryngii strains, specifically screened by the following steps:

[0019] Step 1: using glucose, yeast, agar, and lignin as the first medium; using wort juice, agar, sodium acetate, and potassium chloride as the second medium.

[0020] Step 2: Take a corresponding part of the first culture medium and the second culture medium obtained in step 1 as the third culture medium.

[0021] Step 3: Transfer the first culture medium, the second culture medium and the third culture medium to the aseptic culture vessel respectively, and then inoculate the Pleurotus eryngii strains to be screened into the first culture medium, the second culture medium and the third culture medium respectively. on the third medium.

[0022] Step 4: Continue to cultivate the Pleurotus eryngii strains in the three mediums in step 3. When the strain grows to 5mm, select medium-large, plump, white, and shiny strains for each culture medium. strain.

...

Embodiment 2

[0030] A method for screening the rejuvenation of Pleurotus eryngii strains, specifically screened by the following steps:

[0031] Step 1: using glucose, yeast, agar, and lignin as the first medium; using wort juice, agar, sodium acetate, and potassium chloride as the second medium.

[0032] Step 2: Take a corresponding part of the first culture medium and the second culture medium obtained in step 1 as the third culture medium.

[0033] Step 3: Transfer the first culture medium, the second culture medium and the third culture medium to the aseptic culture vessel respectively, and then inoculate the Pleurotus eryngii strains to be screened into the first culture medium, the second culture medium and the third culture medium respectively. on the third medium.

[0034] Step 4: Continue to cultivate the Pleurotus eryngii strains in the three mediums in step 3. When the strain grows to 6mm, select medium-to-large, plump, white, and shiny strains for each culture medium. strain....

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Abstract

The invention discloses a pleurotus eryngii spawn rejuvenation screening method which specifically comprises the following screening steps: 1, taking glucose, yeasts, agar and xylogen as a first culture medium, and taking wort, agar, sodium acetate and potassium chloride as a second culture medium; 2, respectively correspondingly taking one part of the first culture medium and the second culture medium obtained in the step 1 as a third culture medium; and 3, respectively transferring the first culture medium, the second culture medium and the third culture medium into sterile culture dishes, and respectively inoculating to-be-screened pleurotus eryngii spawns onto the first culture medium, the second culture medium and the third culture medium. By continuously recording and regulating thegrowth environment and detecting different culture media, the pleurotus eryngii spawns that are the most exceptional and have the highest fermentation potency and the best growth can be obtained. Therefore, screening of the pleurotus eryngii is realized.

Description

technical field [0001] The invention belongs to the technical field of screening for rejuvenation of Pleurotus eryngii, in particular to a screening method for rejuvenation of Pleurotus eryngii strains. Background technique [0002] Pleurotus eryngii has declined due to long-term transplantation and subculture on artificial culture, and the invasion of pathogens has affected the growth of the strains, making the growth of Pleurotus eryngii longer in the future, and the quality of formation is also lower, which greatly affects The benefits of mushroom cultivation. Contents of the invention [0003] The purpose of the present invention is to provide a method for screening the rejuvenation of Pleurotus eryngii bacterial strains, to solve the problem that the Pleurotus eryngii proposed in the above-mentioned background technology is due to the long-term artificial culture. The slippage of the seed propagation makes the growth of Pleurotus eryngii more long in the future, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 卓智勇陈长茂周勤亮
Owner 江苏品品鲜生物科技股份有限公司
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