A rapid hydroponic screening method for the tolerance of mulberry varieties to heavy metal cadmium

Abstract

The invention discloses the water-planting rapid screening method of the tolerance of mulberry varieties to heavy metal cadmium. A mulberry is used as repairing plant and is planted through a water planting method. The heavy metal cadmium with different concentrations is added to a water planting solution so as to carry out stress treatment. A linear regression method is used to establish the linear regression equation of a heavy metal cadmium stress concentration and a mulberry water planting death rate. According to the linear regression equation, the variety tolerance index of the mulberryvarieties to a heavy metal cadmium element is calculated. According to the medial lethal concentration and the lethal concentration of water planting, the mulberry varieties were compared and screenedto obtain the mulberry varieties with the higher or lower tolerance to the heavy metal cadmium. The method has characteristics that operation is simple, cost is low, a screening period is short, environmental pollution is low and so on. Soilless culture can save fertilizer and water consumption, is clean and sanitary, and labor saving, and is easy to manage. The method is not limited by a region,a space is fully used, and the method gets rid of the constraints of a natural environment. Uniformity and repeatability required for a test can be ensured.

Description

technical field [0001] The invention belongs to the technical field of mulberry seed breeding and ecological application, and in particular relates to a hydroponic rapid screening method for tolerance of mulberry varieties to heavy metal cadmium. Background technique [0002] Phytoremediation technology is an important means to utilize and treat heavy metal-contaminated soil, and the selection of remediation plants is the key. Most of the known heavy metal hyperaccumulator plants have small biomass, slow crop growth, limited root system, and have a certain restoration effect on shallow soil, but the restoration rate is slow and the distribution is strong regionally; superrich Accumulating plants often only have the enrichment effect on a certain heavy metal, but most of the heavy metal pollution in the soil is compound pollution, the remediation cycle is long, and the practical application is difficult. Mulberry is resistant to heavy metal complex pollution, and has mature ...

AI Technical Summary

Problems solved by technology

Nevertheless, the mulberry soil has a long growth cycle, and there are many mulberry species resources. It is a large workload and a long cycle to screen heavy metal-tolerant or sensitive species, which is easy to cause secondary pollution to the environment, which is not conducive to the realization of high-efficiency and non-toxic mulberry planting and sericulture. Ecological applications such as utilization and remediation of heavy metal-contaminated soil

The existing screening mainly adopts the pot plant method, but this method has difficulties in consistency of the substrate, irrigation, and addition tests, which affects the repeatability of the test, and the test period is long, which is easy to cause secondary pollution