Method for increasing biomass of bacicllus natto at high temperature

A technology of biomass and Bacillus natto, which is applied in the field of bioengineering, can solve the problems of affecting the physiological activity of cells, reducing the biomass of Bacillus natto, inhibiting the growth performance and metabolic activity of bacteria, and achieving the improvement of biomass and good growth performance , effective and rapid biomass effect

Active Publication Date: 2019-01-11
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The data show that Bacillus natto has strong heat resistance, up to 50°C, but the high temperature environment seriously inhibits the growth performance and metabolic activity of the bacteria, affects the physiological activity of cells, and reduces the biomass of Bacillus natto

Method used

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  • Method for increasing biomass of bacicllus natto at high temperature
  • Method for increasing biomass of bacicllus natto at high temperature
  • Method for increasing biomass of bacicllus natto at high temperature

Examples

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preparation example Construction

[0013] Preparation of corn peptide aqueous solution: Weigh a certain amount of plump corn kernels and grind them into corn flour, put them into a 1L Erlenmeyer flask, and use phosphate buffer (concentration: 0.1moi / L, PH=6.8) to make a mass fraction of 5%. The corn flour suspension, stirred evenly, put it into a constant temperature water bath shaker to 55 ° C, and adjusted the pH value to 8.5; add alkaline protease according to the enzyme amount of 4% of the mass ratio of the enzyme to the corn flour suspension, and hydrolyze for 1.5h ; Maintain the pH value at 8.5 with 4mol / L NaOH aqueous solution. Put the triangular flask in a 90°C water bath and keep it for 15 minutes to inactivate the enzyme; take out the triangular flask, rapidly cool down at room temperature, centrifuge at 4000r / min for 10min to get the supernatant, filter it through a 0.22μm filter membrane, freeze-dry the filtrate to obtain corn peptide powder.

[0014] The corn peptide powder is dissolved in sterile...

Embodiment 1

[0017] Example 1 (G1)

[0018] A method for improving the biomass of Bacillus natto under high temperature conditions, comprising the steps of:

[0019] (1) Take 10ul of Bacillus natto glycerol stock solution preserved at -20°C and inoculate it in 50ml seed culture medium, and culture it at 37°C on a shaker at 200r / min for 6h; The corn peptide aqueous solution with a concentration of 0.15g / ml was cultured on a shaker at 200r / min at 37°C for 6h to obtain a seed solution;

[0020] (2) The seed solution was inoculated in the fermentation medium at an inoculum amount of 2%, and cultured on a shaker at 200 r / min at 53° C. for 30 h.

[0021] Embodiment 1 is 3 parallel.

Embodiment 2

[0031] Example 2 (G2)

[0032] A method for improving the biomass of Bacillus natto under high temperature conditions, comprising the steps of:

[0033] (1) Take 10ul of Bacillus natto glycerol stock solution preserved at -20°C and inoculate it in 50ml seed culture medium, and cultivate it at 37°C for 4h on a shaker at 150r / min; A corn peptide aqueous solution with a concentration of 0.2g / ml was cultured on a shaker at 150r / min at 37°C for 4 hours to obtain a seed solution;

[0034] (2) The seed solution was inoculated in the fermentation medium at an inoculation amount of 1%, and cultured on a shaker at 150 r / min at 50° C. for 36 hours.

[0035] Embodiment 2 is 3 parallel.

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Abstract

The invention discloses a method for increasing the biomass of bacicllus natto at a high temperature. The method comprises the following steps: culturing bacicllus natto in a seed culture medium for 4-8 h, adding a corn peptide aqueous solution with the volume being 0.2-0.6% of that of the seed culture medium and the concentration being 0.1-0.2 g / ml, and carrying out culture on a shaking table with the rotating speed of 150-250 r / min at 37 DEG C for 4-8 h to obtain a seed solution; and inoculating the seed solution into a fermentation culture medium according to the inoculation concentration of 1%-3%, and carrying out culture on the shaking table with the rotating speed of 150-250 r / min at 50-55 DEG C for 24-36 h. The seed solution pretreated by corn peptides with different contents is introduced to the fermentation culture medium, and therefore, the bacicllus natto has better growth property than a control group (untreated by the corn peptides) under a high temperature (50-55 DEG C) stress. The method disclosed by the invention is simple, easy and capable of effectively and rapidly increasing the biomass of the bacicllus natto at a high temperature of 50-55 DEG C.

Description

technical field [0001] The invention relates to a method for increasing the biomass of natto bacteria under high temperature conditions, and belongs to the technical field of bioengineering. Background technique [0002] Natto, originated in ancient China, is made from soybeans fermented by Bacillus natto. It is sticky, smells bad, and tastes slightly sweet. Natto has gradually become a favorite nutritional food and health food for people all over the world. It has high nutritional value, and its protein, vitamin B, vitamin E, calcium and iron are higher than ordinary soybeans. Natto also has many physiological health effects, such as anti-cancer effect, anti-oxidation effect, lowering blood pressure, preventing and treating osteoporosis, promoting blood coagulation, antibacterial effect and thrombolytic effect. [0003] Bacillus natto, also known as Bacillus natto, belongs to the subspecies of Bacillus subtilis natto. Industrial production of natto requires Bacillus natto...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12R1/07
CPCC12N1/20C12N1/38
Inventor 王罡孙培季静张亚楠
Owner TIANJIN UNIV
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