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Method for realizing hematological reconstruction by virtue of IBMI of fetal liver Ficoll separated unicell suspension

A single-cell suspension, single-cell technology, applied in extracellular fluid diseases, animal cells, vertebrate cells, etc., can solve problems such as inability to obtain HSCs effectively

Inactive Publication Date: 2019-01-11
SHANGHAI CHILDRENS HOSPITAL +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, neither in vitro differentiation nor transdifferentiation methods can effectively obtain HSCs with hematopoietic reconstruction function.

Method used

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  • Method for realizing hematological reconstruction by virtue of IBMI of fetal liver Ficoll separated unicell suspension
  • Method for realizing hematological reconstruction by virtue of IBMI of fetal liver Ficoll separated unicell suspension
  • Method for realizing hematological reconstruction by virtue of IBMI of fetal liver Ficoll separated unicell suspension

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Embodiment 1

[0017] Experimental animals: HG transgenic mice: Erythroid high-expression GFP transgenic mice, using the fetal liver of this mouse as donor cells, traced peripheral blood GFP + The GFP gene in bone marrow cells can be identified by cell or DNA PCR, which can detect the dynamic ratio change of donor cells in blood and the chimerism in bone marrow cells. Mating with C57 and C57, the fetal liver of E13.5 fetal mouse obtained, and the mononuclear cells obtained by Ficoll separation were used as donor cells. Recipients were 45.1 / 2 mice. C57 mice: Partial fetal liver donor mice and recipient mice transplanted with fetal liver HSCs. 45.1 / 1 mice were purchased from Shanghai Nanfang Model Animal Center and mated with C57 to obtain the fetal liver of E13.5 fetal mice, and the mononuclear cells obtained by Ficoll separation were used as donor cells. Recipient mice: 654 thalassemia mice, purchased from Jakeson Company.

[0018] Reagents: hematoxylin-eosin (BASO BA4025, Zhuhai, China);...

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Abstract

The invention discloses a method for realizing hematological reconstruction by virtue of IBMI of a fetal liver Ficoll separated unicell suspension. By virtue of operation steps of fetal liver unicellsuspension preparation and fetal liver HSCs transplantation, The method is cpable of efficiently reconstructing a blood system, achieving safe, stable and reliable hematopoiesis, effectively treatingthalassemia mice with high quality, and ensuring that the hematologic parameter and pathologic index of the treated mice are approximate to a normal individual, thus reserving a technical system basisfor future clinical exploitation on application of fetal liver hematopoietic stems in efficient treatment on blood diseases.

Description

technical field [0001] The invention belongs to the technical field of hematopoietic stem cell preparation, and in particular relates to a method for IBMI injecting fetal liver Ficoll-separated single-cell suspension to rebuild hematopoiesis. Background technique [0002] Hematopoietic stem cells (HSCs) are a type of cells that can self-renew and differentiate into various blood cell lines. HSCs transplantation can treat related diseases such as malignant hematological diseases, genetic metabolic diseases and congenital immunodeficiency. At present, the sources of hematopoietic stem cells are mainly bone marrow HSCs and umbilical cord blood HSCs, but the source of donor cells is limited, resulting in an extremely limited number of patients who can be treated. Therefore, there is an urgent clinical need to explore new types of transplantable hematopoietic stem cells. In addition to obtaining HSCs from individuals in vivo, other researches are more on the formation of HSCs th...

Claims

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Application Information

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IPC IPC(8): C12N5/0786A61K35/28A61P7/00A61P7/06
CPCA61P7/00A61P7/06A61K35/28C12N5/0645
Inventor 曾凡一黄淑帧杨冠恒
Owner SHANGHAI CHILDRENS HOSPITAL
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