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A kind of ethinyl estradiol degrading bacteria and its method for remediating polluted soil

A technology for ethinyl estradiol and contaminated soil, which is applied in the field of ethinyl estradiol-degrading bacteria and its repair of contaminated soil, can solve the problems of high repair cost and limited repair range, and achieve the promotion of plant growth, extraction and enrichment, Improve the effect of the repair effect

Inactive Publication Date: 2020-08-18
ZHEJIANG GONGSHANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Physicochemical treatment technology is mainly used in the pretreatment of sewage treatment plants and water purification devices of water purifiers. When this method is used in soil remediation, the remediation cost is high and the remediation range is limited.

Method used

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  • A kind of ethinyl estradiol degrading bacteria and its method for remediating polluted soil
  • A kind of ethinyl estradiol degrading bacteria and its method for remediating polluted soil
  • A kind of ethinyl estradiol degrading bacteria and its method for remediating polluted soil

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Embodiment Construction

[0023] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0024] An ethinyl estradiol-degrading bacterium, including: the ethinyl estradiol-degrading bacterium is a Hyphomicrobium genus with the preservation number CCTCC NO: M2017539, named Hyphomicrobium sp.GHH, and the preservation unit is: CCTCC-China Typical Culture Collection Center, Address: Wuhan, China, date of deposit: September 25, 2017.

[0025] △ The screening and identification process of bacteria is as follows:

[0026] Soil samples were taken from a farmland in Hangzhou in October 2016. Weigh 5g of soil and suspend in 100mL inorganic salt medium (MSM) (NH 4 Cl 2g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.2g / L, FeSO 4 ·7H 2 O 0.01g / L, CaCl 2 0.03g / L, MnSO 4 ·H 2 O 0.001g / L, ZnSO4 0.001g / L, pH 7), cultivated in a shaker at 120rpm and 30°C for 24h. Add 4 mg / L of EE2 as the sole carbon source, and after...

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Abstract

The invention discloses ethinyl estradiol degrading bacteria and a method for repairing polluted soil thereof. The ethinyl estradiol degrading bacteria belong to Hyphomicrobium with preservation number CCTCC NO: M2017539, and the name of the ethinyl estradiol degrading bacteria is Hyphomicrobium sp. GHH. According to the ethinyl estradiol degrading bacteria and the method for repairing the polluted soil thereof, the ethinyl estradiol degrading bacteria are separated from farmland, and the bacteria can remove ethinyl estradiol in soil; and ryegrass and the ethinyl estradiol degrading bacteria are combined to repair the ethinyl estradiol polluted soil, the degrading bacteria promote the growth of plants and the extraction and enrichment of ethinyl estradiol by the plants, so that the repaireffect of the plants on the ethinyl estradiol polluted soil is significantly improved.

Description

technical field [0001] The invention relates to the field of microbial treatment of the environment, in particular to an ethinyl estradiol-degrading bacterium and a method for repairing polluted soil. Background technique [0002] Environmental endocrine disruptors (EEDCs) and their effects on human and animal endocrine systems are receiving more and more attention. Ethinyl estradiol, a typical environmental endocrine disruptor, is also an active ingredient in many contraceptives. At the same time, it is also used in the treatment of prostate cancer and breast cancer, menopause and postmenopausal syndrome, osteoporosis and other diseases. [0003] Ethinyl estradiol enters the environment through human or animal excreta. Farmland is the main source of food, and irrigation, runoff, and livestock and poultry manure will make ethinyl estradiol continue to enter the farmland. Studies have shown that ethinyl estradiol can be rapidly adsorbed in soil and sediment. In addition, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10B09C1/00
CPCB09C1/10B09C1/105B09C2101/00
Inventor 何闪英郭海慧李渊吕黎柴奇伟倪瑶琪曹梦卓
Owner ZHEJIANG GONGSHANG UNIVERSITY
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