Construction method of influenza B virus tree shrew model

Abstract

The invention provides a construction method of an influenza B virus tree shrew model. The method comprises the following steps: anesthetizing the tree shrew; The anesthetized tree shrews were inoculated with influenza B virus nasally, to detect the indexes of tree shrews.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for constructing a tree shrew model of influenza B virus. Background technique [0002] Influenza virus is a single negative-sense RNA virus that causes influenza in humans and animals, and can be divided into A (A), B (B), C (C), and D (D) influenza viruses (White SK, Ma W, McDaniel CJ, Gray GC, Lednicky JA. Serologic evidence of exposure to influenza D virus among persons with occupational contact with cattle. J Clin Virol. 2016 Aug; 81:31-3.). Due to its strong virulence, influenza A virus can cause high morbidity and mortality. It is the main cause of previous influenza outbreaks and has brought disastrous consequences to humans (Fukuyama S, Kawaoka Y. The pathogenesis of influenza Virus infections: the contributions of virus and host factors. Curr Opin Immunol. 2011; 23(4):481-6. Taubenberger JK, Reid AH, Janczewski TA, [0003] Fanning TG. Integratin...

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Problems solved by technology

From 2003 to 2008, surveys in some parts of China showed that the mortality rate caused by influenza B virus infection was higher than that of seasonal A / H3N2 or A / H1N1 viruses, and more than half of influenza-related mortality was related to influenza B virus (Feng L, Shay DK, Jiang Y, Zhou H, Chen X, Zheng Y, Jiang L, Zhang Q, Lin H, Wang S, Ying Y, Xu Y, Wang N, Feng Z, Viboud C, Yang W, Yu H .Influenza-associated mortality in temperate and subtropical Chinese cities, 2003-2008.Bull World Health Organ.2012Apr 1; ​​90(4):279-288B.), simultaneous infection with influenza B virus can increase the risk of secondary severe bacterial infection, for Prevention and Treatment of Influenza B Virus Cannot Be Ignored

In the currently used model animals, ferrets exhibit influenza-like symptoms and immune responses similar to humans after infection with influenza virus (Banner D, Kelvin AA. The current state of H5N1 vaccines and the use of the ferret model for influenza therapeutic and prophylactic development .J InfectDev Ctries.2012Jun 15;6(6):465-9.), is considered to be a relatively suitable small animal model for the study of influenza infection, but a difference was found in the process of using the ferret model to study the pathogenic mechanism of influenza B There are large differences between the results of the studies. In one study, ferrets infected with B / Victoria and B / Yamagata sublines of virus only had mild clinical symptoms and mildly elevated body temperature (Kim YH, Kim HS, Cho SH ,Seo SH.Influenza B virus causes milderpathogenesis and weaker inflammatory responses in ferrets than influenzaAvirus.Viral Immunol.2009Dec; 22(6):423-30.), while research by Stephen S.H et al. shows that certain influenza B strains B / Brisbane / 60 / 2008 Infection of ferrets can lead to persistent infection of the lower respiratory tract and moderate to severe clinical symptoms (Huang SS, Banner D, Paquette SG, Leon AJ, Kelvin AA, Kelvin DJ. Pathogenic influenza B virus in the ferret model establishes lower respiratory tract infection. J Gen Virol. 2014 Oct; 95(Pt 10): 2127-39.)

At the same time, the price of the ferret model is expensive, and the lack of corresponding availability of immune reagents to analyze the immune response of influenza virus is also its disadvantage.

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