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A kind of preparation method of ginsenoside rh2

A technology of ginsenoside and ethanol solution, which is applied in the field of preparation of ginsenoside Rh2, and can solve the problems of poor stability and waste of diol saponins

Active Publication Date: 2020-05-26
YUNNAN YUNKE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Diol saponins in Panax notoginseng saponins are less stable than triols, and are prone to degradation during hot reflux extraction or long-term storage, especially in solutions with methanol or ethanol as solvents. , At present, the standard product of ginsenoside RB1 has been frozen and stored in an ice box for sale
At present, the total saponins of Panax notoginseng are prepared industrially. After the total saponins are eluted, the resin is directly treated with acid and alkali. Ginsenosides Rh2 and RG3 are then eluted and discarded, resulting in waste.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation method steps of this ginsenoside Rh2 are as follows:

[0023] (1) Take 2.2 tons of Panax notoginseng cuts as raw material, grind them to 50 mesh, and then use 70% methanol solution for hot reflux extraction for 3 times, the extraction temperature is 80°C, and the extraction time is 2 hours each time. Concentrate under pressure to recover the organic solvent to obtain a turbid liquid, which is left to stand at 2°C for 24 hours, and filtered to obtain the water-soluble part and the precipitated part of the crude extract of Panax notoginseng;

[0024] (2) Pass the water-soluble part of the crude extract of Panax notoginseng in step (1) through a styrene nonpolar macroporous adsorption resin (HPD100) column to absorb the total saponins of Panax notoginseng, wash the resin column with water until it is colorless, and then use 70% volume concentration Analyze the total saponins of Panax notoginseng with ethanol solution. After the total saponins ...

Embodiment 2

[0029] Embodiment 2: the preparation method steps of this ginsenoside Rh2 are as follows:

[0030] (1) Take 2.0 tons of Panax notoginseng cut as raw material, crush it to 60 mesh, and then use methanol solution with a mass concentration of 75% to heat reflux extract twice, the extraction temperature is 85°C, and the extraction time is 3 hours each time, filter, combine the extracts, reduce Concentrate under pressure to recover the organic solvent to obtain a turbid liquid, which is left to stand at 3°C ​​for 24 hours, and filtered to obtain the water-soluble part and the precipitated part of the crude extract of Panax notoginseng;

[0031] (2) Pass the water-soluble part of the crude extract of Panax notoginseng in step (1) through a styrene nonpolar macroporous adsorption resin (D101) column to absorb the total saponins of Panax notoginseng, wash the resin column with water until it is colorless, and then use 75% volume concentration of The ethanol solution was used to analyz...

Embodiment 3

[0036] Embodiment 3: the preparation method steps of this ginsenoside Rh2 are as follows:

[0037] (1) Take 1 ton of Panax notoginseng cut as raw material, crush it to 70 mesh, and then use 80% ethanol solution for hot reflux extraction for 3 times, the extraction temperature is 82°C, each extraction time is 2.5h, filter and combine the extracts, Concentrate under reduced pressure to recover the organic solvent to obtain a turbid liquid, which is left to stand at 2°C for 24 hours, and filtered to obtain the water-soluble part and the precipitated part of the crude extract of Panax notoginseng;

[0038] (2) Pass the water-soluble part of the crude extract of Panax notoginseng in step (1) through a styrene nonpolar macroporous adsorption resin (XAD-1600) column to absorb the total saponins of Panax notoginseng, wash the resin column with water until it is colorless, and then use a volume concentration of 72 % ethanol solution to analyze the total saponins of notoginseng, after t...

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PUM

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Abstract

The invention discloses a preparation method of ginsenoside Rh2. The method takes wastes in a production and preparation process of total panax notoginseng saponins as raw materials to prepare the 20(S)-ginsenoside Rh2 with the purity greater than 98 percent through a process of macroporous resin column enrichment and impurity removal, extraction, separation, recrystallization and the like. The method provided by the invention is simple and easy to operate and suitable for industrialized production; the aim of sufficiently utilizing waste resources is realized.

Description

technical field [0001] The invention relates to a preparation method of ginsenoside Rh2, which belongs to the field of preparation of plant active ingredients. Background technique [0002] The molecular weight of ginsenoside Rh2 is 622.88. Ginsenoside Rh2 mainly plays an anti-tumor effect by blocking the synthesis and metabolism of some important enzymes in the biological reaction system; it can inhibit the growth of tumor cells and block the synthesis and metabolism of important components of tumor cells; among them The anticancer activity of 20(S)-ginsenoside Rh2 is higher than that of 20(R)-ginsenoside Rh2. [0003] The content of ginsenoside Rh2 in fresh Panax notoginseng is very small, and notoginseng diol saponins will be degraded to ginsenoside Rh2 in varying degrees during the drying or heat reflux extraction process of Panax notoginseng. [0004] Diol saponins in Panax notoginseng saponins are less stable than triols, and are prone to degradation during hot reflux...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 辛文锋蔡群虎刘石磊张文生胡会泽袁敏惠王芬张芳盛郭霞余斌孟维
Owner YUNNAN YUNKE PHARMA