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Arsenic ion detection method based on aptamer chain-black phosphorus nanosheet fluorescence energy resonance transfer

An energy resonance transfer and detection method technology, applied in the field of arsenic ion detection, can solve the problems of high cost, complicated arsenic ion detection steps, time-consuming and labor-intensive, etc., and achieves the advantages of good selectivity, short detection time, and improved detection sensitivity and detection range. Effect

Active Publication Date: 2019-01-18
TAIYUAN UNIV OF TECH
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Problems solved by technology

[0006] Aiming at the problems that the existing detection steps of arsenic ions are complicated, time-consuming and laborious, and costly, the present invention provides a detection method of arsenic ions based on fluorescence energy resonance transfer of aptamer chain-black phosphorus nanosheets

Method used

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  • Arsenic ion detection method based on aptamer chain-black phosphorus nanosheet fluorescence energy resonance transfer
  • Arsenic ion detection method based on aptamer chain-black phosphorus nanosheet fluorescence energy resonance transfer

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Embodiment 1

[0021] A method for detecting arsenic ions based on aptamer chain-black phosphorus nanosheet fluorescent energy resonance transfer, the detection method comprising the following steps:

[0022] Step one, As 3+ Preparation of aptamer chain solution: Take 4 µL of ROX dye-labeled aptamer chain solution with an original concentration of 100 µM, add a certain volume of ultrapure water to prepare a 100 nM aptamer chain solution for later use.

[0023]Step 2. Optimization of the concentration of the black phosphorus nanosheet dispersion: take 1mL of the black phosphorus nanosheet dispersion with an original concentration of 200µg / mL and add four equal parts of the black phosphorus nanosheet dispersion, respectively, to add different volumes of ultrapure water to prepare concentrations of 200µg / mL, 100µg / mL, 50µg / mL, 25µg / mL and 0µg / mL black phosphorus nanosheet dispersions; 40µL of different concentrations of black phosphorus nanosheet dispersions were added to 200µL of 100nM As 3+ ...

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Abstract

The invention relates to an arsenic ion detection method based on aptamer chain-black phosphorus nanosheet fluorescence energy resonance transfer, belonging to the technical field of arsenic ion detection. The method solves problems that existing arsenic ion detection method has complex steps, takes time and effort and is high-cost. According to the detection method, As<3+> in a to-be-detected solution is detected quantitatively according to change of fluorescence strength recovery level of a fluorescent dye ROX in a reaction system by using a fluorescence quenching characteristic of black phosphorus nanosheet and an As<3+> probe comprising black phosphorus nanosheet and As<3+> aptamer chain marked with the fluorescent dye ROX. The detection method has the advantages of fast detection speed, simple detection steps, high detection sensitivity, and high selectivity.

Description

technical field [0001] The invention belongs to the technical field of arsenic ion detection, and in particular relates to an arsenic ion detection method based on fluorescence energy resonance transfer of aptamer chain-black phosphorus nanosheets. Background technique [0002] In recent years, with the development of industrialization, heavy metal pollution has become more and more serious, especially arsenic pollution has aroused people's great concern. Arsenic is a highly toxic carcinogen, among which trivalent arsenic compounds are more toxic than other arsenic compounds, and the toxicity of trivalent arsenic is about 60 times that of pentavalent arsenic. Arsenic pollution is widely distributed, and it is reported that more than 20 countries have suffered from arsenic pollution, which has caused many acute and chronic health problems, such as skin damage, circulatory system diseases and other high-risk diseases. To make matters worse, arsenic cannot be removed from the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6428G01N2021/6417G01N2021/6432G01N2021/6439
Inventor 李晓春李海琴张校亮于化忠
Owner TAIYUAN UNIV OF TECH
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