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Insect antifeedant 13,21-dihydroeurycomanone transported in plants and its application

A technology of insects and plants, applied in the direction of plant growth regulators, applications, insecticides, etc., can solve the problems of loss of drug efficacy, interference effects, etc., and achieve the effects of environmental friendliness, high antifeedant activity, and convenient use

Active Publication Date: 2021-03-23
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some compounds have been found to have antifeedant activity against insects, which are generally applied on the surface of plants to exert antifeedant activity, but this type of antifeedant needs to be sprayed, and it is easily affected by the interference of the external environment after application, such as rainwater washing will lead to loss of efficacy

Method used

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  • Insect antifeedant 13,21-dihydroeurycomanone transported in plants and its application
  • Insect antifeedant 13,21-dihydroeurycomanone transported in plants and its application
  • Insect antifeedant 13,21-dihydroeurycomanone transported in plants and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 113

[0025] Example 113, The plant systemic properties of 21-dihydroeurycomanone and the antifeedant activity after systemic absorption

[0026] Method: Dissolve 13,21-dihydroeurycomanone in methanol, and then make it into a drug solution with a final concentration of 100 μg / mL, and spread it evenly on the surface of a circular cabbage leaf dish with a diameter of 1.2 cm. The dosage is 20 μL, and after the methanol on the surface of the leaf disc is naturally dried, the 3rd instar larvae of the diamondback moth Plutellaxylostella (Linnaeus) are inserted, and each concentration treatment is repeated 3 times at a relative humidity of 50% to 70% and a temperature of 25°C. After keeping in an environment of ~28°C for 24 hours, the feeding area was counted, and the feeding rate and refusal rate were calculated.

[0027] The antifeedant activity of 13,21-dihydroeurycomanone against the last 1st instar larvae of Spodoptera litura (Fabricius) was tested with cassava leaves; the antifeedant...

Embodiment 2

[0034] Example 2 13,21-dihydroeurycomanone can be systemically sucked from the growth environment of A. chinensis through the root to the root of A. chinensis, and transported upwards to the leaves, making insects refrain from eating the leaves of A. chinensis

[0035] Method: 13,21-dihydroeurycomanone was dissolved in DMSO to make a 10000μg / mL mother solution, and then 0.5mM CaCl 2 Dilute the stock solution with aqueous solution to 100 μg / mL culture solution. Put the previously cultivated Shanghai green seedlings (6-leaf stage, not treated with 13,21-dihydroeurycomanone before) in 0.5mM CaCl 2 After pre-cultivation in aqueous solution for 2 hours, transfer it to the prepared 13,21-dihydroeurycomanone culture solution mentioned above. After 24 hours, take 0.7 g of the top leaf of Shanghai green, mash it and dissolve it in 2 mL of methanol, and use liquid chromatography-mass spectrometry The instrument can detect that 13,21-dihydroeurycomanone is contained in the leaf extract,...

Embodiment 3

[0037] Example 3: 13,21-dihydroeurycomanone can be absorbed from the growth environment of Chinese cabbage through the root to the inside of Chinese cabbage root, and transported upward to the leaves, so that the vegetable aphid Lipaphiserysimi refuses to eat the leaves of Chinese cabbage

[0038] Method: 13,21-dihydroeurycomanone was dissolved in methanol to make a mother solution of 10000μg / mL, and then 0.5mM CaCl 2 Dilute the stock solution with aqueous solution to 100 μg / mL culture solution. The previously cultivated cabbage seedlings (6-leaf stage, not treated with 13,21-dihydroeurycomanone before) were placed in 0.5mM CaCl 2 After pre-cultivation in aqueous solution for 2 hours, transfer to the above-mentioned 13,21-dihydroeurycomanone culture solution prepared above, and 24 hours later, insert the aphid without wings, insert 100 heads in each cage, repeat 4 times, and insert the aphid 24 hours later. Count the number of cabbage aphids inhabited on the control leaves an...

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Abstract

The invention discloses an insect anti-feedant 13,21-dihydroeurycomanone conducted in plant bodies and application. The 13,21-dihydroeurycomanone has anti-feeding activity on various insects and can be conducted in the plant bodies; the anti-feeding effect is better than that of an anti-feedant which is applied to the surfaces of plants, and the anti-feedant can be used for preparing a systemic insect anti-feedant; the insect anti-feedant is convenient to use, is not interfered and influenced by an external environment and has a good application prospect; the insect anti-feedant is obtained byseparating and purifying natural plants, and has low toxicity, no residues, no pollution and no pollution nuisance on economic crops including various grains, melons, vegetables and the like.

Description

technical field [0001] The invention belongs to the fields of biological pesticides and plant protection, and in particular relates to insect repellent 13,21-dihydroeurycomanone transported in plants and its application. Background technique [0002] Agricultural pests occur in a large area in many areas of our country, and the frequency is on the rise, and the damage is aggravated. At present, chemical pesticide control is still the main measure. Due to the long-term use of chemical pesticides for prevention and control, the pollution of land, water and air, the emergence of plant resistance, and the significant decline in the control effect have forced farmers to increase the amount of pesticides used to improve the control effect, but this has reduced the safety of agricultural products. Therefore, it is particularly important to explore efficient, safe and environment-friendly green pesticides. The development of botanical pesticides has always been a research hotspot ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01N25/04A01P7/04
CPCA01N25/04A01N43/90
Inventor 邵雪花赖多匡石滋肖维强田永清凡超
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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