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An insect repellant that can be transported in plants

An antifeedant and plant technology, applied in the field of pesticides, can solve problems such as loss of efficacy and interference effects, and achieve the effects of convenient use and good application prospects.

Active Publication Date: 2021-05-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, this type of antifeedant needs to be sprayed, and it is easily affected by the interference of the external environment after application, such as the loss of efficacy due to rainwater erosion, etc.

Method used

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  • An insect repellant that can be transported in plants
  • An insect repellant that can be transported in plants
  • An insect repellant that can be transported in plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Test the antifeedant activity of Brucea brucein D on several kinds of leaf-eating Lepidoptera larvae that damage vegetables with the classic leaf disc drug-loading method

[0045] 1. Method

[0046] Dissolve Brucea javanica D with methanol, and then apply the prepared drug solution evenly on the surface of a circular cabbage leaf disc with a diameter of 1 cm. The amount of drug applied to each leaf disc is 20 μL. After drying, insert the 2nd instar larvae of the diamondback moth Plutellaxylostella (Linnaeus), repeat each concentration treatment 3 times, and keep it in an environment with a relative humidity of 50% to 70% and a temperature of 25°C to 28°C for 24 hours before counting feeding area to calculate feeding rate and refusal rate.

[0047] Cassava leaves were used to test the antifeedant activity of brucein D on the end-1st instar larvae of Spodoptera litura (Fabricius), and amaranth was used to test the antifeedant activity of brucein D on the end-1...

Embodiment 2

[0057] Example 2: Brucea javanica D can be sucked into the root of Chinese cabbage through the root from the growth environment of Chinese cabbage, and transported upward to the leaves, so that insects refuse to eat the leaves of Chinese cabbage

[0058]Dissolve brucein D in methanol to make a 10000μg / mL mother solution, and then add 0.5mM CaCl 2 Dilute the stock solution with aqueous solution to 100 μg / mL culture solution. The previously cultivated cabbage seedlings (6-leaf stage, not previously treated with Brucella D) were placed in 0.5mM CaCl 2 After pre-cultivation in aqueous solution for 2 hours, transfer it to the above-mentioned prepared Brucea javanica D culture solution. After 24 hours, take 0.5 g of the top leaves of Chinese cabbage, mash it and dissolve it in 2 mL of methanol. It can be detected by high-performance liquid chromatography. This extract contains brucein D, and the content of brucein D in the top leaves (fresh leaves) of Chinese cabbage reaches 40 μg / ...

Embodiment 3

[0059] Embodiment 3: Brucella bruceae D can be sucked into the cabbage root from the growth environment of Chinese cabbage through the root, and transported upwards to the leaves, making Phyllotreta striolata (Fabricius) adults refuse to eat the cabbage leaves

[0060] Dissolve brucein D in methanol to make a 10000μg / mL mother solution, and then add 0.5mM CaCl 2 Dilute the mother liquor with aqueous solution to a 50 μg / mL culture broth. The previously cultivated cabbage seedlings (6-leaf stage, not previously treated with Brucella D) were placed in 0.5mM CaCl 2 After pre-cultivation in aqueous solution for 2 hours, transfer it to the above-mentioned prepared Brucea javanica D culture solution. After 24 hours, insert 50 flea beetle adults into each cage, repeat 4 times, and insert flea beetle adults. After 24 hours, A counts the feeding area, and calculates the average food refusal rate by the formula in Example 1 to be 71%.

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Abstract

The invention discloses the application of brucella D in the preparation of insect antifeedant and an insect antifeedant which can be conducted in plants. The research of the present invention finds that Coriolus D has antifeedant activity on various insects, and Corvusine D has systemic properties and can be transported in plants, and the antifeeding effect is better than the antifeedant applied on the surface of plants, which can be used for preparing The systemic insect repellent is more convenient to use, is not affected by the interference of the external environment, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of pesticides. More specifically, it relates to the application of an insect repellent that can be transported in plants, ie Brucea brucein D, in the preparation of the insect repellent. Background technique [0002] Insect repellent refers to a specific agent that interferes with or inhibits the function of the taste receptors of insects so that they do not eat. This type of chemical substance can generally prevent insects from feeding, but will not directly kill insects. Using insect repellents to protect plants has great advantages, not only preventing plants from being eaten by insects, but also helping to protect the ecology balance, so insect repellents are ideal insect repellents in plant protection. [0003] At present, some compounds have been found to have antifeedant activity against insects, generally by applying them on the surface of plants to exert antifeedant activity. [0004] However, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01N25/04A01N25/12A01P7/04
CPCA01N25/04A01N25/12A01N43/90
Inventor 徐汉虹田永清毛根林黄志升
Owner SOUTH CHINA AGRI UNIV
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