Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method and application of autologous collagen stock solution used in cooperation with microneedles

A technology of pro-collagen and collagen, which is applied in the field of preparation of autologous collagen stock solution with microneedles, can solve problems such as skin rejection and skin damage, and achieve the effect of solving rejection and ensuring compatibility.

Inactive Publication Date: 2019-01-29
白晋
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the embodiments of the present invention is to provide a preparation method and application of autologous collagen stock solution with microneedles, so as to solve the problem that the existing microneedles are prone to produce rejection reactions to the skin and cause damage to the skin when used

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This embodiment discloses a method for preparing autologous collagen stock solution with microneedles, including the following steps:

[0023] Step 1. Soak the mature autologous fibrous tissue in ultrapure water, stirring continuously until it becomes a paste; crush the paste-like autologous fibrous tissue and put it into 10 times the volume of ultrapure water to obtain the autologous fibrous tissue Suspension, add glacial acetic acid to the autologous fibrous tissue suspension to prepare a mixture, and the concentration of the mixture is 0.4mg / mL, set aside;

[0024] Step 2, prepare pepsin solution, and the preparation method of pepsin solution comprises first weighing pepsin, then pepsin is dissolved in 0.5M acetic acid to make the pepsin solution of 10mg / mL, in the mixture prepared in step one Add pepsin solution to carry out the digestion reaction. After the digestion reaction is over, let it stand for stratification, put the upper suspension in a centrifuge with a ...

Embodiment 2

[0028] This embodiment discloses a method for preparing autologous collagen stock solution with microneedles, including the following steps:

[0029] Step 1. Soak the mature autologous fibrous tissue in ultrapure water, stirring continuously until it becomes a paste; crush the paste-like autologous fibrous tissue and put it into 10 times the volume of ultrapure water to obtain the autologous fibrous tissue Suspension, add glacial acetic acid to the autologous fibrous tissue suspension to prepare a mixture, and the concentration of the mixture is 0.4mg / mL, set aside;

[0030] Step 2, prepare pepsin solution, and the preparation method of pepsin solution comprises first weighing pepsin, then pepsin is dissolved in 0.5M acetic acid to make the pepsin solution of 10mg / mL, in the mixture prepared in step one Add pepsin solution to carry out the digestion reaction. After the digestion reaction is over, let it stand for stratification, put the upper suspension in a centrifuge with a ...

Embodiment 3

[0034] This embodiment discloses a method for preparing autologous collagen stock solution with microneedles, including the following steps:

[0035] Step 1. Soak the mature autologous fibrous tissue in ultrapure water, stirring continuously until it becomes a paste; crush the paste-like autologous fibrous tissue and put it into 10 times the volume of ultrapure water to obtain the autologous fibrous tissue Suspension, add glacial acetic acid to the autologous fibrous tissue suspension to prepare a mixture, and the concentration of the mixture is 0.4mg / mL, set aside;

[0036] Step 2, prepare pepsin solution, and the preparation method of pepsin solution comprises first weighing pepsin, then pepsin is dissolved in 0.5M acetic acid to make the pepsin solution of 10mg / mL, in the mixture prepared in step one Add pepsin solution to carry out the digestion reaction. After the digestion reaction is completed, let it stand for stratification, put the upper layer suspension into a centr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The embodiment of the invention discloses a preparation method and application of an autologous collagen stock solution used in cooperation with microneedles. The preparation method of the autologouscollagen stock solution used in cooperation with the microneedles includes the steps of preparation of autologous fibrous tissue suspension liquid, performing of digestion reaction, salting out and final synthesis. According to the preparation method of the autologous collagen stock solution used in cooperation with the microneedles, the autologous collagen stock solution is adopted so that the problems that the microneedles are prone to having rejection reaction with skin and harming the skin can be effectively solved, and compatibility of tissues in organisms is guaranteed. In addition, theinvention provides the microneedles which can be effectively matched with the autologous collagen stock solution for use, so that great convenience is brought to people.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of medical cosmetology, and in particular to a preparation method and application of an autologous collagen stock solution with microneedles. Background technique [0002] Microneedle beauty is a very fashionable beauty method. It uses many tiny needles on the microneedle roller to stimulate the skin. In a short period of time, microneedle can make more than 200,000 microscopic channels. By stimulating the regeneration of collagen, it can achieve Cosmetic purposes. However, when the existing microneedle is used, it is easy to produce a rejection reaction to the skin and cause damage to the skin, which is unfavorable for people to use. Contents of the invention [0003] The purpose of the embodiment of the present invention is to provide a preparation method and application of using autologous collagen stock solution with microneedles, so as to solve the problem that the existing ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K8/65A61K8/02A61K8/73A61K8/365A61Q19/00
CPCA61Q19/00A61K8/0204A61K8/365A61K8/65A61K8/735A61K2800/91A61K2800/805A61K2800/10A61M37/0015
Inventor 白晋
Owner 白晋