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Hemsleya amabilis novel disinfection means

A new type of technology for snow gallbladder, which is applied in the field of new disinfection methods for snow gallbladder, can solve the problems of inconspicuous effect, time-consuming and inconvenient, and large-scale production of snow gallbladder expansion and cultivation, and achieve the effect of shortening the cultivation period

Inactive Publication Date: 2019-02-12
云南善源生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing disinfection methods are single, such as using annual branches as explants, hydroponics indoors, after germination and growth of new branches, as explants for hydroponics, rinsing the branches with running water, and cutting them into 5-6 cm The stems with long axillary buds are soaked in 75% alcohol for 2-5 seconds, and then treated with 0.1% mercury chloride for 5 minutes. This method is time-consuming and inconvenient, and the effect is not obvious, which leads to the large-scale production of snow gall expansion and cultivation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0019] A new disinfection method for snow gallbladder, using potassium permanganate solution to disinfect snow gallbladder, comprising the following steps:

[0020] a. Pretreatment: intercept the stem section of the twig of snow gall as the outer finger body, cut the branch into a stem section with a length of 5 cm, remove excess leaves, and put it in a glass bottle for subsequent use;

[0021] b. Preparation of disinfectant solution: mix potassium permanganate and sterile water to prepare a disinfectant solution with a concentration of 0.1%;

[0022] c. Soaking: Pour the disinfectant solution prepared in b into a glass bottle with the stems of snow gall, and replace it with new disinfectant solution after soaking for 1.5 hours, for a total of 3 times.

[0023] The snow gall stems soaked in step c do not need to be rinsed with sterile water. The snow gall stems treated in steps a-c are put into MS medium for propagation and cultivation. MS medium is currently the most commonly...

Embodiment example 2

[0025] A new disinfection method for snow gallbladder, using potassium permanganate solution to disinfect snow gallbladder, comprising the following steps:

[0026] a. Pretreatment: intercept the stem section of the twig of snow gall as the outer finger body, cut the branch into a stem section with a length of 5 cm, remove excess leaves, and put it in a glass bottle for subsequent use;

[0027] b. Prepare disinfectant solution: mix potassium permanganate and sterile water to prepare a disinfectant solution with a concentration of 0.2%;

[0028] c. Soaking: Pour the disinfectant solution prepared in b into the glass bottle with the stems of snow gall, and replace it with new disinfectant solution after soaking for 0.5 hours for a total of 3 times.

[0029] The snow gall stems soaked in step c do not need to be rinsed with sterile water. The snow gall stems treated in steps a-c are put into MS medium for propagation and cultivation. MS medium is currently the most commonly used ...

Embodiment example 3

[0031] A new disinfection method for snow gallbladder, using potassium permanganate solution to disinfect snow gallbladder, comprising the following steps:

[0032] a. Pretreatment: intercept the stem section of the twig of snow gall as the outer finger body, cut the branch into a stem section with a length of 5 cm, remove excess leaves, and put it in a glass bottle for subsequent use;

[0033] b. Prepare disinfectant: mix potassium permanganate and sterile water to prepare a disinfectant with a concentration of 0.15%;

[0034] c. Soaking: Pour the disinfectant prepared in b into a glass bottle containing the stems of the snow gall, and replace it with a new disinfectant after soaking for 1 hour, for a total of 4 times.

[0035] The snow gall stems soaked in step c do not need to be rinsed with sterile water. The snow gall stems treated in steps a-c are put into MS medium for propagation and cultivation. MS medium is currently the most commonly used medium. It has a higher co...

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Abstract

The invention discloses a hemsleya amabilis novel disinfection means. A potassium permanganate solution is utilized to conduct disinfection treatment on hemsleya amabilis, and the means comprises thefollowing steps of pre-treatment, in specific, the twig stem section of hemsleya amabilis is cut to serve as an explant, the branch is cut to be stem sections with the length being 5 cm, redundant leaves are removed and the stem sections are placed into a glass bottle for standby application; preparation of disinfection liquid, in specific, potassium permanganate is mixed with sterile water and conditioned to be the disinfection liquid with the concentration being 0.1-0.2%; and dipping, in specific, the well produced disinfection liquid is poured into the glass bottle filled with the hemsleyaamabilis stem sections, the glass bottle is replaced with new disinfection liquid after the hemsleya amabilis stem sections are dipped for every 0.5-1.5 hours, and dipping is conducted for 3-5 times.Compared with the prior art, the novel disinfection means has the advantages that the original hemsleya amabilis disinfection means that water culture and reproduction need to be conducted firstly, and then disinfection is conducted is modified to be the means that the hemsleya amabilis twig stem sections are directly cut and disinfected with the potassium permanganate solution, the culture periodof sterile hemsleya amabilis stem sections is greatly shortened, potassium permanganate makes less hurt to the plant and has good sterilization effect, and good basis is provided for following propagation expanding and culturing of hemsleya amabilis.

Description

technical field [0001] The invention relates to the technical field of snow gall cultivation, in particular to a novel disinfection method for snow gall. Background technique [0002] Existing snow gall tissue culture mostly utilizes the stem section with leaf buds to proliferate, and the growth cycle is 45-60 days. In the process of tissue culture of snow gallbladder, callus is very easy to produce, resulting in the situation that the seedlings do not grow buds, or the stems die directly. Therefore, disinfection and sterilization before the expansion and culture are important links in the whole tissue culture process. Disinfection Whether it is thorough is directly related to the follow-up work. Existing methods of disinfection are single, such as using annual branches as explants, hydroponics indoors, after germination and growth of new branches, as explants for hydroponics, rinsing the branches with running water, and cutting them into 5-6 cm Stems with long axillary bu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 陈玲
Owner 云南善源生物科技发展有限公司