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High ammonia-nitrogen heterotrophic nitrification-aerobic denitrification resisting ochrobactrum and application thereof

A technology of aerobic denitrification and Paleobacterium pallidum, applied in the field of environmental microorganisms, to achieve the effect of simple medium composition, strong tolerance to high salinity, and low cost

Active Publication Date: 2019-02-15
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The genus Pallidum is a type of bacterial group that is obligate to aerobic, strict respiratory metabolism, and can use various amino acids, organic acids and carbohydrates as carbon sources. There is no literature record that Pallidum genus has heterotrophic nitrification-aerobic denitrification Function

Method used

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  • High ammonia-nitrogen heterotrophic nitrification-aerobic denitrification resisting ochrobactrum and application thereof
  • High ammonia-nitrogen heterotrophic nitrification-aerobic denitrification resisting ochrobactrum and application thereof
  • High ammonia-nitrogen heterotrophic nitrification-aerobic denitrification resisting ochrobactrum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Pale bacterium Ochrobacterum sp. TAC-2 at the ammonia nitrogen concentration of 400 mg / L ammonia nitrogen and total nitrogen removal ability measurement experiment

[0038] Prepare bacillus pallidum culture medium with an ammonia nitrogen concentration of 400 mg / L, take 100 mL of bacillus pallidum culture medium in a 250 mL Erlenmeyer flask, sterilize it by high-temperature steam at 121 ℃ for 30 min, and use a micropipette after cooling Add 2 mL of bacillus pallidum (OD 600 nm For 1-2), use a parafilm to seal, put in a shaker and set at 30 ℃, 150 r·min -1 Culture under the conditions, and then measure the OD of the bacterial solution every 24 hours 600 nm The value determines the growth of the bacteria, and at the same time determines the content of ammonia nitrogen and total nitrogen in the medium to determine the removal effect of ammonia nitrogen and total nitrogen. by figure 1 It can be seen that Pale bacillus Ochrobacterum sp. TAC-2 completely removes 4...

Embodiment 2

[0039] Example 2 Pale bacillus Ochrobacterum sp. TAC-2 on the removal effect of different concentrations of ammonia nitrogen

[0040] Prepare bacillus pallidum culture media with ammonia nitrogen concentrations of 100 mg / L, 200 mg / L, 400 mg / L, 600 mg / L, 800 mg / L, and 1000 mg / L, and take 100 mL of bacillus pallidus with different ammonia nitrogen concentrations. The culture is based on a 250 mL Erlenmeyer flask, which is sterilized by high-temperature steam at 121 ℃ for 30 min. After cooling, use a micropipette to add 2 mL of Palebacterium 600 nm For 1-2), use a parafilm to seal, put in a shaker and set at 30 ℃, 150 r·min -1 Culture under the conditions, and then measure the OD of the bacterial solution every 24 hours 600 nm The value determines the growth of the bacteria, and at the same time determines the ammonia nitrogen content in the culture medium to determine the ammonia nitrogen removal effect. by figure 2 It can be seen that when the ammonia nitrogen concentration is...

Embodiment 3

[0041] Example 3 Pale bacterium Ochrobacterum sp. TAC-2 high ammonia nitrogen removal effect experiment under different salinity conditions

[0042] Prepare bacillus pallidum culture media with salinity of 1%, 2%, 3%, 4%, and 5% respectively. The ammonia nitrogen concentration of each bacillus pallidum culture medium is 400 mg / L. Take 100 mL of different salinity bacillus pallidus. The culture is based on a 250 mL Erlenmeyer flask, which is sterilized by high-temperature steam at 121 ℃ for 30 min. After cooling, use a micropipette to add 2 mL of Palebacterium 600 nm For 1-2), use a parafilm to seal, put in a shaker and set at 30 ℃, 150 r·min -1 Culture under the conditions, and then measure the OD of the bacterial solution every 24 hours 600 nm The value determines the growth of bacteria, and at the same time measures the content of ammonia nitrogen in the medium to determine the removal effect of ammonia nitrogen. by image 3 It can be seen that when the salinity is less than...

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Abstract

The invention relates to a high ammonia-nitrogen heterotrophic nitrification-aerobic denitrification resisting ochrobactrum TAC-2 (Ochrobacterum sp.TAC-2) with a preservation number of CCTCC NO: M 2018028. The ochrobactrum can realize the process of synchronous nitrification and denitrification in an aerobic environment under the conditions of high ammonia nitrogen and high salinity and effectively removes total nitrogen from high ammonia-nitrogen wastewater and high-salinity wastewater; the ochrobactrum is especially suitable for removing ammonia-nitrogen from the high ammonia-nitrogen wastewater and has wide popularization significance.

Description

Technical field [0001] The invention belongs to the technical field of environmental microbes, and specifically relates to a pallor bacillus resistant to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification and its application. Background technique [0002] In recent years, the discharge of high ammonia nitrogen wastewater (ammonia nitrogen concentration> 200 mg / L) has been increasing. Improper treatment of high-ammonia-nitrogen wastewater directly discharged into the water body will cause the eutrophication of the water body to become more and more serious and cause the deterioration of the water body quality. In order to control ammonia nitrogen pollution in water bodies, my country has promulgated new strict pollutant control standards, among which the first-level standard for ammonia nitrogen is 5.0 mg / L. The new standard puts forward new requirements for the effective control of ammonia nitrogen, especially the effective control of ammonia nitrogen i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 赵天涛艾铄罗晓静刘向阳张千项锦欣
Owner CHONGQING UNIV OF TECH
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