Reagent kit and method for quickly detecting polymorphism of hypertension individualized medication genes

Abstract

The invention discloses a reagent kit and a method for quickly detecting the polymorphism of hypertension individualized medication genes, belongs to the technical field of gene detection, and relatesto clinical anti-hypertension medicine beta 1 receptor blocker, angiotensin II receptor 1 antagonist and angiotensin converting enzyme inhibitor medicine metabolism. The reagent kit comprises sampletreatment reagents, premixed and individual-portion separately packaged gene detection reagent PCR (polymerase chain reaction) liquid 1, PCR liquid 2, PCR liquid 3, positive reference substances and negative reference substances. The sample treatment reagents are used for quickly treating human whole-blood samples. The reagent kit and the method have the advantages that the polymorphism of the hypertension individualized medication genes can be conveniently, quickly and efficiently detected by the aid of the reagent kit and the method on the basis that the detection specificity and sensitivityare guaranteed, detection operation steps can be reduced, the detection reaction time can be shortened, the production cost and the detection cost can be reduced, and the reagent kit and the method are favorable for clinical detection and analysis application.

Description

technical field [0001] The invention relates to a kit and method for gene detection, in particular to a kit and method for rapidly detecting gene polymorphism of individualized medication for hypertension, belonging to the technical field of gene detection. Background technique [0002] CYP2D6 only accounts for 1-2% of the total CYP in the liver, but there are more than 80 kinds of drugs catalyzed and metabolized by it. The activity of CYP2D6 in different individuals can vary by up to 1000 times. The most common polymorphism in the Chinese population is CYP2D6* 10. About 51.6% of Chinese people carry this genetic variation. This polymorphism leads to a decrease in the enzyme activity of CYP2D6 and is extremely unstable, thereby affecting drug metabolism. [0003] CYP2C9 is an important member of the cytochrome P450 family, accounting for 20% of the total liver microsomal P450. This enzyme catalyzes about 12% of commonly used clinical drugs. There are polymorphisms in the CYP...

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Problems solved by technology

[0007] The present invention detects CYP2D6*10, CYP2C9*3, ADRB1 1165G>C, AGTR1 1166A>C, and ACE I / D gene polymorphisms in the prior art, which requires high sample processing, long detection period, complicated operation and high cost In order to solve the deficiencies such as low specificity and low specificity, a kit and method for rapid detection of genetic polymorphisms in individualized medication for hypertension are provided, involving antihypertensive drugs β1 receptor blockers, angiotensin Ⅱ receptor 1 antagonists, Angiotensin-converting enzyme inhibitor drug metabolism, receptor sensitivity-related CYP2D6*10, CYP2C9*3, ADRB1 1165G>C, AGTR1 1166A>C, ACE I / D gene polymorphism detection kit and method

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