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Optimization method of digital image plane micro-hologram

A technology of holographic microscopy and optimization method, applied in the field of signal processing, can solve the problems of no improvement, the reliability of reconstruction phase and the decline of precision, and achieve the effects of eliminating influence, improving stability and reliability, and improving signal-to-noise ratio.

Inactive Publication Date: 2019-02-19
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

For the image plane hologram, although the reconstruction process avoids the noise introduced by the traditional digital holographic diffraction calculation, it does not improve the reliability and accuracy of the reconstruction phase due to the noise introduced by the recording environment and recording devices during the recording process.

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Embodiment Construction

[0034] It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0035] The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0036] The digital image plane holographic micrograph is the holographic interferogram recorded on the focusing imaging plane of the microscopic objective lens in the digital holographic microscopic system. The intensity of the image plane hologram is

[0037] I(x,y)=O(x,y) 2 +R(x,y) 2 +O * R(x,y)+OR * (x,y) (1)

[0038] In the formula, O(x,y) is the complex amplitude of the object light wave, R(x,y) is the complex amplitude of the reference light wave, O(x,y) 2 +R(x,y) 2 is the zero-order image, O * R(x,y)+OR * (x, y) represent real and virtual images. The zero-order image, real image and virtual image respectively occupy three parts in the spectrogram of the image plane holog...

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Abstract

The invention discloses an optimization method of a digital image plane micro-hologram. The accuracy and the reliability of axial measurement are limited by noise led in by a recording process in a digital holographic microscopy technique. An existing noise restraint method is mainly researched according to high-frequency noise. According to the optimization method, recorded high-frequency noise and recorded low-frequency noise are restrained, and the signal to noise ratio of a reconstruction phase is improved. According to the optimization method, by the aid of digital image plane holographicmicroscopy technique, an empirical mode decomposition method is combined, and a hologram is decomposed and optimized. A standard nano-step surface appearance is measured by the optimization method, frequency spectrums of the hologram before and after optimization and a measured height graph are compared and analyzed, results show that reliable measurement of a micro-structural surface appearanceis ensured by the optimization method, and the signal-to-noise ratio of the reconstruction phase of the digital image plane holographic microscopy technique is improved.

Description

technical field [0001] The invention belongs to the technical field of signal processing, in particular to a method for optimizing a digital image plane holographic micrograph. Background technique [0002] Digital Image-plane Holographic Microscopy (DIPHM) is a special case of Digital Holographic Microscopy (DHM). By recording the hologram on the imaging surface of the microscope objective lens, a holographic micrograph of the digital image surface is obtained. Compared with traditional digital holographic micrographs, reconstruction of image plane holographic micrographs does not require diffraction calculations, so it has the advantages of low noise, high efficiency, and true full-field reconstruction. For the image plane hologram, although the reconstruction process avoids the noise introduced by the traditional digital holographic diffraction calculation, it does not improve the problem of the reliability and accuracy of the reconstruction phase caused by the noise int...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G03H1/00G03H1/04G03H1/08G02B21/36
CPCG02B21/365G02B21/367G03H1/0005G03H1/041G03H1/0443G03H1/0866G03H2001/005G03H2001/0447G03H2001/0454G03H2001/0471G03H2210/10G03H2210/12G03H2223/13G03H2223/52G03H2223/55G03H2226/13
Inventor 曾雅楠刘源吴海云胡晓东卢钧胜常新宇
Owner TIANJIN AGRICULTURE COLLEGE