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Brady-growing soybean rhizobia strains, compositions and uses

A technology of soybean rhizobia and composition, applied in the field of microorganisms, can solve problems such as failure to meet storage conditions and poor farmland effects

Active Publication Date: 2019-12-24
康生元(肇庆)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, maintaining the survival of microorganisms during seed treatment and storage is a key issue, but currently there are few commercially available rhizobium agents that can attach to seeds with strong application performance, long shelf life and good stability [10]
And because the storage conditions of most microbial products are always not up to the optimal storage conditions (for example, high temperature, light, humidity, etc.), the effect of farmland is not good

Method used

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  • Brady-growing soybean rhizobia strains, compositions and uses
  • Brady-growing soybean rhizobia strains, compositions and uses
  • Brady-growing soybean rhizobia strains, compositions and uses

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Preliminary screening of desiccation-resistant rhizobia

[0048] In this study, 2,000 strains of symbiotic nitrogen-fixing root nodules isolated from different regions of 26 provinces (cities) across the country were used as the strain resource bank. According to the inevitable relationship between the drought tolerance of strains and their ecological environment, this study finally selected the isolates 260 rhizobia strains from Northwest China, North China, Northeast China, Central China, East China, Southwest China and South China were used as research materials, and their anti-drying ability was initially evaluated. Bacterial activity was determined based on the fluorescent staining method (LIVE / DEAD Bacterial Viability Kit) of the cell membrane combined with glass beads vacuum drying method, and the anti-drying strains were initially screened.

[0049] The main method is to randomly select 20 fields of view on the fluorescent microporous filter membrane with a s...

Embodiment 2

[0083] Field experiment data of 5038 strain and other soybean rhizobia strains

[0084] 1. Experimental site 1: Changchun Academy of Agricultural Sciences, with an experimental area of ​​560m 2 .

[0085] Different soybean rhizobia strains (5038 and 5841 strains) were used to dress the soybean seeds, and the soybean seeds after seed dressing were sown in the normal way. During the harvest period, the soybeans with different treatments were tested for plant height, pod number per plant, and The calculation of the number of grains per plant, grain weight per plant and 100-grain weight index, the results (see Table 3) show that soybean rhizobium strain 5038 is significantly better than the control and soybean rhizobium strain 5841.

[0086] Table 3 The effect of rhizobia inoculation in soybean harvest period

[0087]

[0088] 2. Experimental site 2: Liangshang experimental site of Chifeng Academy of Agriculture and Animal Husbandry Science, sandy loam

[0089] Materials use...

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Abstract

The invention relates to the field of microorganisms, in particular to a brady-growing soybean rhizobium strain, composition and application. The strain is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, the preservation number is: CGMCC No: 16747; the preservation time is: November 15, 2018. It is highly resistant to desiccation and has a good ability to nodulate and fix nitrogen with soybeans.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a brady-growing soybean rhizobium strain, composition and application. Background technique [0002] Leguminous plants-rhizobia is a classic model of biological nitrogen fixation, and the symbiotic system formed by it is the system with the highest biological nitrogen fixation efficiency in nature, which plays an important role in increasing crop yields, reducing the use of chemical fertilizers, reducing water and soil pollution, and achieving sustainable agricultural development [1] . Environmental factors such as salinity, temperature, pH, drought, and heavy metal ions are important factors that affect the infection process of rhizobium-legume symbiotic nitrogen fixation, the development and function of root nodules, and lead to low crop yields with less nitrogen fixation. [2] . Drought, in particular, restricts the biological nitrogen fixation capacity of rhizobia-legumes in ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01C1/06A01C1/00C12R1/01
CPCA01C1/00A01C1/06C12N1/20C12N1/205C12R2001/01
Inventor 朱杰李俊康耀卫
Owner 康生元(肇庆)生物科技有限公司