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Application of miR-6802-3p to early diagnosis of postmenopausal osteoporosis

An osteoporosis, women's technology, applied in the field of biomedicine, can solve problems such as induced diseases and abnormal gene expression, achieve the effect of timely microdiagnosis and reduce mortality

Inactive Publication Date: 2019-02-22
王冉东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The abnormal expression of miRNA directly leads to the abnormal expression of some genes related to the occurrence of diseases, which induces the occurrence of diseases.

Method used

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  • Application of miR-6802-3p to early diagnosis of postmenopausal osteoporosis
  • Application of miR-6802-3p to early diagnosis of postmenopausal osteoporosis
  • Application of miR-6802-3p to early diagnosis of postmenopausal osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Sequencing screening of miRNAs associated with osteoporosis in postmenopausal women

[0049] 1. Research object and sample collection

[0050] Three postmenopausal women with osteoporosis, three normal postmenopausal women, and normal postmenopausal women served as controls. The clinical information is shown in Table 1.

[0051] Table 1 Clinical information of research subjects

[0052]

[0053] The research subjects were required to fast for at least 12 hours, and at room temperature from 7:00 to 8:00 the next morning, 10ml of venous blood was drawn into EDTA anticoagulant tubes to extract peripheral blood mononuclear cell PBMCs, and 1ml of Trizol reagent was added (Invitrogen Company), mix thoroughly, and store the specimen at -80°C for RNA extraction. All blood samples and pathological results should be authentic and reliable, the study was approved by the ethics committee, and the patients gave informed consent.

[0054] 2. RNA extraction from blood...

Embodiment 2

[0082] Example 2 QPCR verification of differentially expressed miR-6802-3p

[0083] 1. According to the sequencing results in Example 1, miR-6802-3p was selected for large sample QPCR verification. According to the sample collection method in Example 1, 30 normal postmenopausal women and 30 postmenopausal women with osteoporosis were selected to separate and store blood mononuclear cells.

[0084] 2. The RNA extraction process is the same as in Example 1.

[0085] 3. Reverse transcription: mix 10pg-1μg total RNA template with 2μl 10* buffer, 2μl dATP (10mM), 0.5μl polyA polymerase, 0.5μl ribonuclease (RNase) inhibitor and ribonuclease free water (RNase free water ) were mixed, the final volume was 20 μl, and incubated at 37° C. for 1 h. Then add 1 μl 0.5 μg / μl Oligo(dT) specific RT primer to the reaction tube, incubate at 70°C for 5 minutes and immediately incubate on ice for at least 2 minutes to break the secondary structure of RNA and primers. Finally, mix the above 20 μ...

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Abstract

The invention discloses an application of miR-6802-3p to early diagnosis of postmenopausal osteoporosis. MiRNA with differential expression between a postmenopausal osteoporosis patient and a normal control is screened out by sequencing, then large sample QPCR experiments prove that content of miR-6802-3p in blood of the postmenopausal osteoporosis patient is substantially up-regulated definitely,so that miR-6802-3p can serve as a molecular marker for diagnosing postmenopausal osteoporosis to be used for developing products for diagnosing postmenopausal osteoporosis.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of miR-6802-3p in the early diagnosis of osteoporosis in postmenopausal women. Background technique [0002] Osteoporosis (OP) is a systemic bone disease characterized by low bone mass, destruction of bone microarchitecture, increased bone fragility, and susceptibility to fracture (WHO). In 2001, the National Institutes of Health (NIH) of the United States proposed that osteoporosis is a disease of the skeletal system characterized by decreased bone strength and increased risk of fracture. Bone strength reflects two main aspects of bones, namely bone mineral density and bone quality. . [0003] The disease can occur in different genders and at any age, but it is more common in postmenopausal women and older men. Osteoporosis is divided into primary and secondary two categories. Primary osteoporosis is further divided into three types: postmenopausal osteoporosis (type I...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/118C12Q2600/178
Inventor 王冉东
Owner 王冉东
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