Sample density separation liquid for liquid-based cytological slide preparation

Abstract

The invention discloses a sample density separation liquid for liquid-based cytological slide preparation. The sample density separation liquid comprises, by mass, 6%-11% of glycerin, 2%-4% of anhydrous ethanol, 3%-5% of sodium chloride, 79.6%-89.3% of distilled water and 0.4%-0.7% of tea saponin. The sample density separation liquid makes cells of diagnostic significance naturally settle at a faster speed and reduces the sedimentation interference of red blood cells and inflammatory cells, so a microscopic examination slice is allowed to have a clearer visual field for microscopic examinationand good cell staining effect; and the sample density separation liquid can be widely applied to pretreatment of various gynecological and non-gynaecological exfoliative cytology specimens and manual, semi-automatic or fully-automatic liquid-based cytological slide preparation.

Description

technical field [0001] The invention relates to the field of pretreatment of liquid-based cytology samples in biomedical technology, in particular to a sample density separation liquid used for liquid-based cell slice preparation. Background technique [0002] Cervical cancer is one of the most common malignant tumors in women and the main killer of women's health. Its incidence rate is second only to breast cancer. According to statistics, there are about 500,000 new cases of cervical cancer in the world every year, of which 80% occur In developing countries, early screening of cervical cancer is therefore particularly important. [0003] At present, the commonly used early screening method for cervical cancer is liquid-based cytology, which uses a liquid-based thin-layer cytology detection system to detect cervical cells and conduct cytological classification diagnosis. It is currently a more advanced cervical cancer cell in the world. Compared with the traditional cervic...

AI Technical Summary

Problems solved by technology

[0005] The traditional commonly used sample density separation liquid is Ficoll separation liquid. The main component of Ficoll separation liquid is a synthetic sucrose polymer, referred to as polysucrose. The red blood cells that have not been completely lysed by the preservation solution at the layered part of the separation solution will be in contact with polysucrose to form a string of money and deposit at the bottom of the tube to mix with the sinking cells of diagnostic significance (epithelial cells, tumor cells), affecting Observation of samples after subsequent film production