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A kind of serum miRNA marker and the method for detecting ionizing radiation damage thereof

A technology of ionizing radiation and markers, applied in the field of biomarkers, can solve the problems of unsatisfactory timeliness, convenience, stability and sensitivity, etc., achieve reliable and accurate detection results, and improve specificity and sensitivity

Active Publication Date: 2021-10-08
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Claims
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Problems solved by technology

At present, the main biomarkers detected by ionizing radiation include chromosomal aberrations in lymphocytes, cell micronuclei, and radiation-sensitive protein molecules, etc., but these markers still have deficiencies in terms of timeliness, convenience, stability, and sensitivity. ideal

Method used

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  • A kind of serum miRNA marker and the method for detecting ionizing radiation damage thereof
  • A kind of serum miRNA marker and the method for detecting ionizing radiation damage thereof
  • A kind of serum miRNA marker and the method for detecting ionizing radiation damage thereof

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Embodiment Construction

[0031] A serum miRNA marker, which is a combination of miR-134-5P and miR-155-5P.

[0032] Among them: MiR-134-5P maturation sequence is 5'- ugugacugguugaccagagggggg -3 '.

[0033] The mature sequence of MIR-155-5P is 5'-uuaaughuaauugugauagggu-3 '.

[0034] The serum miRNA marker detects the method of electroplated radiation damage, including the following steps:

[0035] (1) Total RNA is extracted from normal individual serum samples.

[0036] Due to the low abundance of RNA in serum samples, it can be purified by Qiagene's Mirneasy Serum / Plasma Kit kit. Before the extraction, foreign nematodes Cel-miR-39 were added as internal paramers, and the normalization process of data after the PCR reaction was used. The specific procedure is as follows:

[0037] 1 Milled serum samples, add 5 times the Qiazol Lysis Reagent of the sample, and the vortex is mixed. 1 ml Qiazol Lysis Reagent is required such as 200 ul serum.

[0038] 2 Deposition at room temperature for 5 min, add 3.5 ul Cel...

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Abstract

The present invention relates to a serum miRNA marker, which is a combination of miR-134-5p and miR-155-5p. At the same time, the invention also discloses a method for detecting ionizing radiation damage by the serum miRNA marker. Compared with traditional chromosomal aberrations and protein biomarkers, the serum miRNA markers of the present invention are more stable in the extraction and storage process, making the detection results more reliable and accurate; and verified by qRT-PCR method, its expression level and radiation The dose is also closely related, which can provide a reliable and accurate assessment basis for judging whether the test object is at risk of being damaged by ionizing radiation.

Description

Technical field [0001] The present invention relates to the field of biomarkers, and more particularly to a serum miRNA marker and a method of detecting radiation injury. Background technique [0002] Ionospheric radiation is widely present in the space environment and nuclear adjusted facilities. On the one hand of ionizing radiation, it can act directly on the DNA, protein molecules, and artifacts, etc., resulting in structural damage and functional damage, on the other hand, can also act in a water molecule in cells and metamples, producing a large amount of strong oxidation resistance The free radical indirectly causes damage to the cell. Isolation radiation exceeding secure dose limits may result in acute radiation symptoms such as skin necrosis, retinal and lens lesions, digestive system damage, hematopoietic and immune system injury and organ failure, and also significantly increase the risk of cancer. With the rapid development of nuclear energy, radiation medicine, and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/158
Inventor 危文俊王菊芳华君瑞何进鹏
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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